| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Molecular mechanisms underlying pollen killer system remain largely unknown. A pollen killer gene, S24, and its partner gene EFS have been found in an inter-subspecific cross of Oryza sativa ssp. indica and japonica. To understand molecular mechanism of the pollen killer system by S24-EFS genes, genetic dissection and molecular cloning of these genes were conducted. The location of the EFS locus was narrowed down within 35-kb region. Transformation of the genomic DNA fragment carrying the primary candidate ORF was performed, but there was no significant effect on pollen sterility due to S24. The S24 regions were further characterized and genetically dissected. As a result, a novel gene (INK) close to the S24 locus was identified. Our results showed that INK causes the pollen sterility by interacting with another pollen killer S35 but is independent of both the EFS and S24 genes.
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