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Effective gene analysis system using DNA transposon, nDart

Research Project

Project/Area Number 25450013
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Science in genetics and breeding
Research InstitutionNational Institute for Basic Biology

Principal Investigator

Tsugane Kazoo  基礎生物学研究所, 多様性生物学研究室, 助教 (50343744)

Co-Investigator(Renkei-kenkyūsha) Tsugane Mika (Hayashi Mika)  基礎生物学研究所, 多様性生物学研究室, 特別協力研究員 (90625592)
Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsイネ / トランスポゾン / 転移因子 / 変異創生 / 優性変異 / 突然変異体 / 突然変異 / 逆遺伝学
Outline of Final Research Achievements

To analyze functions of unknown genes of rice, it is required to develop effective gene analysis system which carry the new mutaion. Rice endogenous DNA transposon, nDart elements were non-autonomous element which were transposed by transpoase protein expressed by an autonoumous aDart1-27. Most of insertion sites identifed of nDart were found at genic region. nDart/aDart1-27 system was induced into a rice variety Koshihikari, named as nDart-Koshi-Tag line. The rice endogenous DNA transposon nDart elements were successfuly exploited as mutant line. 3000 MK-1 plants per year were grown for three years. For reverse genetic analysis, identification of insertion sites of nDart have advanced.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (10 results)

All 2016 2015 2014 2013

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (8 results)

  • [Journal Article] A gain-of-function Bushy dwarf tiller 1 mutation in rice microRNA gene miR156d caused by insertion of the DNA transposon nDart12015

    • Author(s)
      Hayashi-Tsugane, M., Maekawa M., Tsugane K.
    • Journal Title

      Scientific Reports

      Volume: 5 Issue: 1 Pages: 14357-14357

    • DOI

      10.1038/srep14357

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] A mutable albino allele in rice reveals that formation of thylakoid membranes requires SNOW-WHITE LEAF1 gene.2014

    • Author(s)
      Hayashi-Tsugane M, Takahara H, Ahmed N, Himi E, Takagi K, Iida S, Tsugane K, Maekawa M.
    • Journal Title

      Plant Cell Physiol.

      Volume: 55 Issue: 1 Pages: 3-15

    • DOI

      10.1093/pcp/pct149

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] DNAトランスポゾンnDart1の遺伝子への挿入によって生じるイネの優性変異の解析2016

    • Author(s)
      栂根一夫、栂根美佳、前川政彦
    • Organizer
      日本遺伝学会88回大会
    • Place of Presentation
      静岡県三島市 日本大学国際関係学部
    • Year and Date
      2016-09-07
    • Related Report
      2016 Annual Research Report
  • [Presentation] Comprehensive analysis of genes affecting cellular DNA ploidy in rice2015

    • Author(s)
      Mika Tsugane, Chikako Inoue, Kazuo Tsugane, Ryohei Terauchi, Nobuhiro Nagasawa, Masahiko Maekawa, Masaki Ito
    • Organizer
      56回日本植物生理学会
    • Place of Presentation
      東京都世田谷区東京農業大学
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Research-status Report
  • [Presentation] Analysis of genes affecting cellular DNA ploidy in rice2014

    • Author(s)
      Mika Tsugane, Chikako Inoue, Kazuo Tsugane, Ryohei Terauchi, Nobuhiro Nagasawa, Masahiko Maekawa, Masaki Ito
    • Organizer
      37回日本分子生物学会年会
    • Place of Presentation
      神奈川県横浜市パシフィコ横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Research-status Report
  • [Presentation] Semidominant gain-of-function mutation in rice microRNA gene caused by nDart insertion2014

    • Author(s)
      Kazuo Tsugane,Mika Hayashi-Tsugane,Masahiko Maekawa
    • Organizer
      55回日本植物生理学会年会
    • Place of Presentation
      富山県、富山大学 五福キャンパス
    • Related Report
      2013 Research-status Report
  • [Presentation] A mutable albino allele in rice reveals that formation of thylakoid membranes requires SNOW-WHITE LEAF1 gene2014

    • Author(s)
      Mika Hayashi-Tsugane,Masahiko Maekawa,Kazuo Tsugane
    • Organizer
      55回日本植物生理学会年会
    • Place of Presentation
      富山県、富山大学 五福キャンパス
    • Related Report
      2013 Research-status Report
  • [Presentation] イネのDNA トランスポゾン、nDart1 の転移に係わる自律性因子の探索2014

    • Author(s)
      西村 秀希, 吉田 明希子, 栂根 一夫, 前川 雅彦
    • Organizer
      日本育種学会 第125回講演会
    • Place of Presentation
      宮城県、東北大学
    • Related Report
      2013 Research-status Report
  • [Presentation] DNAトランスポゾンnDartを用いた逆遺伝学可能な遺伝子タギングラインの構築2013

    • Author(s)
      栂根一夫、栂根美佳、山口勝司、重信秀治、西村秀希、前川雅彦
    • Organizer
      日本育種学会 第124回講演会
    • Place of Presentation
      鹿児島県、鹿児島大学
    • Related Report
      2013 Research-status Report
  • [Presentation] 栽培イネのトランスポゾン活性を抑制する野生イネ因子の解析2013

    • Author(s)
      栂根一夫、西村秀希、栂根美佳、前川雅彦
    • Organizer
      日本遺伝学会第85回大会
    • Place of Presentation
      神奈川県、慶應義塾大学 日吉キャンパス
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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