Effective gene analysis system using DNA transposon, nDart
| Project/Area Number |
25450013
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Science in genetics and breeding
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| Research Institution | National Institute for Basic Biology |
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Principal Investigator |
Tsugane Kazoo 基礎生物学研究所, 多様性生物学研究室, 助教 (50343744)
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| Co-Investigator(Renkei-kenkyūsha) |
Tsugane Mika (Hayashi Mika) 基礎生物学研究所, 多様性生物学研究室, 特別協力研究員 (90625592)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | イネ / トランスポゾン / 転移因子 / 変異創生 / 優性変異 / 突然変異体 / 突然変異 / 逆遺伝学 |
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| Outline of Final Research Achievements |
To analyze functions of unknown genes of rice, it is required to develop effective gene analysis system which carry the new mutaion. Rice endogenous DNA transposon, nDart elements were non-autonomous element which were transposed by transpoase protein expressed by an autonoumous aDart1-27. Most of insertion sites identifed of nDart were found at genic region. nDart/aDart1-27 system was induced into a rice variety Koshihikari, named as nDart-Koshi-Tag line. The rice endogenous DNA transposon nDart elements were successfuly exploited as mutant line. 3000 MK-1 plants per year were grown for three years. For reverse genetic analysis, identification of insertion sites of nDart have advanced.
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Report
(5 results)
Research Products
(10 results)
