| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
We found momilactone high-sensitive and low-sensitive mutants of Arabidopsis. Large field screening programs and laboratory experiments in many countries have indicated that rice is allelopathic and releases allelochemical(s) into its environment. Genetic studies have shown that momilactone is the most important rice allelochemical. However, there has been no information available for the mode of action of momilactone. Therefore, we investigated the action of momilactone on the growth inhibition. Two momilactone high-sensitive and two low-sensitive mutants of Arabidopsis were identified in 14,585 SALK T-DNA insertion lines. Those mutations were related to flavonoid biosynthetic pathway. Then, the expressions of the genes involved in flavonoid biosynthesis were determined by real-time PCR. The present results indicate that momilactone inhibits the growth of Arabidopsis due to its involvement in the gene expressions in the flavonoid biosynthesis.
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