Research Project
Grant-in-Aid for Scientific Research (C)
All processes of “embryo formation”, “shoot induction” and “shoot multiplication and acclimatization” are important for obtaining DHs in apple anther cultures. It is important for efficient acquisition of DHs to select cultivars with high differentiation and multiplication capability in the processes. ‘Senshu’, ‘Starking Delicious’ and ‘Sekaiichi’ were selected as the cultivars suitable for anther culture. It was clarified that the sampling of flower bud at late “Tight clustre” to early “First pink” stage which mainly contained “early uninucleate” to “late uninucleate” microspore and chilling treatment for 25 to 35 days of flower buds were efficient for increasing embryo formation. The seedlings which used DH as a parent were raised, and it was proven that the simplification of mode of inheritance was possible. Acquired DHs were cultivated in the field, and the individual with fertility was found.
All 2017 2016 2015 2014 2013
All Journal Article (2 results) (of which Int'l Joint Research: 1 results, Peer Reviewed: 2 results, Open Access: 1 results, Acknowledgement Compliant: 1 results) Presentation (10 results) (of which Int'l Joint Research: 1 results)
The Horticulture Journal
Volume: 86 Issue: 1 Pages: 1-10
10.2503/hortj.MI-094
130005312799
Journal of Japanese Society for Horticultural Science.
Volume: 82(2) Pages: 114-124
