Construction of efficient genetic analysis model for apple breeding by production of doubled haploids.

• Project/Area Number: 25450038
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Horticultural science
• Research Institution: Iwate University
• Principal Investigator: KOMORI Sadao 岩手大学, 農学部, 教授 (00333758)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: リンゴ / Malus × domestica Borkh. / 葯培養 / 倍加半数体 / 完全ホモ個体 / 遺伝解析 / 育種素材

Outline of Final Research Achievements

All processes of “embryo formation”, “shoot induction” and “shoot multiplication and acclimatization” are important for obtaining DHs in apple anther cultures. It is important for efficient acquisition of DHs to select cultivars with high differentiation and multiplication capability in the processes. ‘Senshu’, ‘Starking Delicious’ and ‘Sekaiichi’ were selected as the cultivars suitable for anther culture. It was clarified that the sampling of flower bud at late “Tight clustre” to early “First pink” stage which mainly contained “early uninucleate” to “late uninucleate” microspore and chilling treatment for 25 to 35 days of flower buds were efficient for increasing embryo formation. The seedlings which used DH as a parent were raised, and it was proven that the simplification of mode of inheritance was possible. Acquired DHs were cultivated in the field, and the individual with fertility was found.

Research Products

• [Journal Article] Elucidating Cultivar Differences in Plant Regeneration Ability in an Apple Anther Culture (2017)
  • Author(s): Zhang, C., Sato, S., Tsukuni, T., Sato, M., Okada, H., Yamamoto, T., Wada, M., Matsumoto, S., Yoshikawa, N., Mimida, N., Takagishi, K., Watanabe, M., Cao Q. and Komori. S.
  • Journal Title: The Horticulture Journal Volume: 86 Issue: 1 Pages: 1-10
  • DOI: 10.2503/hortj.MI-094
  • NAID: 130005312799
  • ISSN: 2189-0102, 2189-0110
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Effect of the microspore development stage and cold pre-treatment of flower buds on embryoid induction in apple (Malus × domestica Borkh.) anther culture. (2013)
  • Author(s): Zhang, C., Tsukuni, T., Ikeda M., Sato, M., Okada, H., Ohashi, Y., Matsuno, H., Yamamoto, T., Wada, M., Yoshikawa, N., Matsumoto, S., Li, J., Mimida, n., Watanabe, M., Suzuki, A. and Komori, S.
  • Journal Title: Journal of Japanese Society for Horticultural Science. Volume: 82(2) Pages: 114-124
  • Peer Reviewed
• [Presentation] リンゴ品種‘ふじ’の葉切片からのシュート再分化効率の向上に関する研究. (2016)
  • Author(s): 山形 拓・三堀 隼・西谷千佳子・滋田徳美・山本俊哉・和田雅人・渡邉 学・小森貞男.
  • Organizer: 園芸学会
  • Place of Presentation: 東京農業大学農学部厚木キャンパス（神奈川県厚木市）
  • Year and Date: 2016-03-26
• [Presentation] Doubled-haploid plants in apple-attractive applications for breeding and genetics. (2015)
  • Author(s): Yamamoto, T., Kunihisa, M., Nishitani, C., Terakami, S., Komori, S., Sato, M. and Okada, H.
  • Organizer: Eucarpia Symposium on Fruit Breeding and Genetics. 2015.
  • Place of Presentation: The Bologna University. Italy.
  • Year and Date: 2015-06-14
  • Int'l Joint Research
• [Presentation] 交配後２年での開花を実現するためのリンゴの播種期前進の試み． (2015)
  • Author(s): 小森貞男，柳本麻衣，高岸香里，守谷(田中)友紀，和田雅人，山本俊哉，村上政伸， 渡邉 学
  • Organizer: 園芸学会
  • Place of Presentation: 千葉大学西千葉キャンパス
  • Year and Date: 2015-03-28 – 2015-03-29
• [Presentation] Malus hupehensisにおける相転換と枝葉の形態との関係 (2015)
  • Author(s): 高岸香里，守谷(田中)友紀，押野秀美，山本俊哉，和田雅人，渡邉 学，小森貞男
  • Organizer: 園芸学会
  • Place of Presentation: 千葉大学西千葉キャンパス
  • Year and Date: 2015-03-28 – 2015-03-29
• [Presentation] リンゴ台木品種のわい化度が単為生殖性を有するMalus hupehensis実生の生育および開花に及ぼす影響 (2014)
  • Author(s): 守谷(田中)友紀，小森貞男，岩波 宏，花田俊男，本多親子，和田雅人
  • Organizer: 園芸学会
  • Place of Presentation: 佐賀大学本庄キャンパス
  • Year and Date: 2014-09-27 – 2014-09-29
• [Presentation] Malus hupehensisの培養系における葉切片からのシュート再分化条件の検討 (2014)
  • Author(s): 山形 拓，三堀隼人，山本俊哉，阿部和幸，渡邉 学，和田雅人，小森貞男
  • Organizer: 園芸学会
  • Place of Presentation: 佐賀大学本庄キャンパス
  • Year and Date: 2014-09-27 – 2014-09-29
• [Presentation] 葯培養由来倍加半数体品種‘リンゴ中間母本95P6’の交雑F1集団の果実品質 (2014)
  • Author(s): 佐藤 守，國久美由紀，滝田雄基，押野秀美，寺上伸吾，西谷千佳子，山口奈々子，岡田初彦，山本俊哉，小森貞男
  • Organizer: 園芸学会平成26年度春季大会． 園学研. 13(別1): 73.
  • Place of Presentation: 筑波大学第２エリア・第３エリア
• [Presentation] 葯培養由来倍加半数体品種‘リンゴ中間母本95P6’を利用した果実形質のQTL解析． (2014)
  • Author(s): 國久美由紀，押野秀美，寺上伸吾，西谷千佳子，滝田雄基，山口奈々子，岡田初彦，山本俊哉，小森貞男，佐藤 守
  • Organizer: 園芸学会平成26年度春季大会． 園学研. 13(別1): 74.
  • Place of Presentation: 筑波大学第２エリア・第３エリア
• [Presentation] リンゴの葯培養において胚様体の大きさ，胚様体形成時期がシュート形成に及ぼす影響． (2014)
  • Author(s): 張 春芬，道合 了，佐藤聡太，佐藤 守，山本俊哉，森田 泉，壽松木章，渡邉 学，小森貞男
  • Organizer: 園芸学会平成26年度春季大会． 園学研. 13(別1): 259.
  • Place of Presentation: 筑波大学第２エリア・第３エリア
• [Presentation] リンゴ葯培養由来倍加半数体品種‘リンゴ中間母本95P6’を利用したQTL解析に向けての連鎖地図作製． (2013)
  • Author(s): 押野秀美，國久美由紀，寺上伸吾，西谷千佳子，山口奈々子，岡田初彦，佐藤 守，小森貞男，山本俊哉
  • Organizer: 園芸学会平成25年度秋季大会． 園学研. 12(別2): 279.
  • Place of Presentation: 岩手大学上田キャンパス