| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
PEPCK is known as a key regulatory enzyme in the gluconeogenesis pathway in plants. However, its physiological function in whole plant development including fruit sugar accumulation is not fully understood in plants with fresh berry-type fruit. To elucidate this function, we generated transgenic tomato plants in which PEPCK is over-expressed or down-regulated. Detailed characterization of the transgenic lines, it was revealed that PEPCK and gluconeogenesis are involved in the regulation of sugar/acid balance in developing tomato fruit. Additionally, PEPCK expression level also affected initial seedling growth. In the PEPCK-overexpressing lines, exogenous sucrose supply strongly promoted post-germination growth. Those results indicate PEPCK is involved in not only regulation in carbohydrate supply but also its availability in tomato seedling.
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