Identification of aroma component related to the field resistance against citrus canker (Xanthomonas citri subsp. citri) and elucidation for its metabolic regulation in citrus

• Project/Area Number: 25450050
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Horticultural science
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: SHIMADA Takehiko 国立研究開発法人農業・食品産業技術総合研究機構, 果樹茶業研究部門カンキツ研究領域, ユニット長 (10355399)
• Co-Investigator(Kenkyū-buntansha): ENDO Tomoko 国立研究開発法人農業・食品産業技術総合研究機構, 果樹茶業研究部門カンキツ研究領域, 上級研究員 (50355400)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: リナロール / カンキツ / かいよう病 / 抵抗性 / 遺伝子組換え体 / モノテルペン

Outline of Final Research Achievements

Linalool had anti-bacterial activity against citrus canker (Xcc) and is highly accumulated in the leaves and mature fruits of Ponkan mandarin with the field resistance to Xcc. Satsuma mandarin had 3 linalool synthase genes in the genome and their transcription increased in response to wounding and the attacks of bacterial and fungi. Transgenic orange leaves harboring transgene of linalool synthase suppressed the growth of Xcc and accumulated high transcripts of pathogen related genes, indicating that linalool might be involved in the induction of non-host resistance in citrus.

Research Products

• [Journal Article] Characterization of three linalool synthase genes from Citrus unshiu Marc. and analysis of linalool-mediated resistance against Xanthomonas citri subsp. citri and Penicilium italicum in citrus (2014)
  • Author(s): Takehiko Shimada a, Tomoko Endo, Hiroshi Fujii , Ana Rodríguez, Leandro Peña, Mitsuo Omura
  • Journal Title: Plant Science Volume: 229 Pages: 154-166
  • DOI: 10.1016/j.plantsci.2014.09.008
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] カンキツ香気成分遺伝子群の構造解析と代謝改変 (2015)
  • Author(s): 島田武彦
  • Organizer: 園芸学会 小集会
  • Place of Presentation: 千葉大学農学部
  • Year and Date: 2015-03-27
• [Book] 果実日本 (2013)
  • Author(s): 島田武彦
  • Total Pages: 3
  • Publisher: 日本園芸農業協同組合連合会
• [Remarks] リナロールの抗菌活性とウンシュウミカンでのリナロールを合成する遺伝子の発現
  • URL: https://www.naro.affrc.go.jp/project/results/laboratory/fruit/2014/fruit14_s14.html