Analysis of dwarf and constitutive defense responses activated by loss of specific Arabidopsis MAP kinase pathway involved in innate immunity signaling
Project/Area Number |
25450060
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Plant protection science
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Research Institution | Kagawa University |
Principal Investigator |
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | サプレッサー変異体 / MAPキナーゼ経路 / 防御反応 / 矮性 / 抵抗性 / 変異体 / 抵抗性遺伝子 / 細胞死 / 活性酸素種蓄積 / 原因遺伝子同定 |
Outline of Final Research Achievements |
Mitogen-activated protein kinase (MAPK) pathways have a pivotal role in innate immunity signaling in plants. Arabidopsis MEKK1, a MAPK kinase kinase, constitutes specific pathway with MKK1/MKK2 and MPK4. This pathway has an important role in regulation of defense gene expression in response to microbe associated molecular patterns recognition. However, loss of MEKK1 results in constitutive defense responses accompanying severe dwarfism and temperature-dependent cell death. To elucidate the complex mechanism underlying the mekk1 phenotypes, we performed genetic screening to isolate suppressor mutant of the dwarf and defense phenotypes. With the aid of the MutMap analysis, we identified the candidate of causal gene for the mutant. Genetic analysis by producing double mutant with mekk1 or mpk4, smn1 mutation suppressed the dwarf and defense phenotypes of these mutants. These results showed that SMN1 positively contributed to the phenotypes.
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] Mitogen-Activated Protein Kinase Kinase 3 Regulates Seed Dormancy in Barley2016
Author(s)
Shingo Nakamura, Mohammad Pourkheirandish, Hiromi Morishige, Yuta Kubo, Masako Nakamura, Kazuya Ichimura, Shigemi Seo, Hiroyuki Kanamori, Jianzhong Wu, Tsuyu Ando, Goetz Hensel, Mohammad Sameri, Nils Stein, Kazuhiro Sato, Takashi Matsumoto, Masahiro Yano, Takao Komatsuda
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Journal Title
Current Biology
Volume: 26
Issue: 6
Pages: 775-781
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Phosphorylation of D-allose by hexokinase involved in regulation of OsABF1 expression for growth inhibition in Oryza sativa L.2013
Author(s)
Fukumoto, T., Kano, A., Ohtani, K., Inoue, M., Yoshihara, A., Izumori, K., Tajima, S., Shigematsu, Y., Tanaka, K., Ohkouchi, T., Ishida, Y., Nishizawa, Y., Tada, Y., Ichimura, K., Gomi, K., Yoo, SD., Sheen, J., Akimitsu, K.
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Journal Title
Planta
Volume: 237
Issue: 5
Pages: 1379-1391
DOI
Related Report
Peer Reviewed
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[Journal Article] The rare sugar D-allose acts as a triggering molecule of rice defence via ROS generation.2013
Author(s)
Kano, A., Fukumoto, T., Ohtani, K., Yoshihara, A., Ohara, T., Tajima, S., Izumori, K., Tanaka, K., Ohkouchi, T., Ishida, Y., Nishizawa, Y., Ichimura, K., Tada, Y., Gomi, K., and Akimitsu, K.
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Journal Title
Journal of Experimental Botany
Volume: 64
Issue: 16
Pages: 4939-4951
DOI
Related Report
Peer Reviewed
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[Presentation] Functional interplay between NLR protein SMN1 and MEKK1 pathway in Arabidopsis2016
Author(s)
Takagi, M., Graf, A., Greenshields, D., Takagi, H., Shinozaki, K., Terauchi, R., Shirasu, K., Ichimura, K
Organizer
第57回日本植物生理学会年会
Place of Presentation
盛岡
Year and Date
2016-03-18
Related Report
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[Presentation] Loss of function of SMN1 suppressed constitutive defense phenotypes in MEKK1 pathway mutants2016
Author(s)
Hamano, K., Takagi, M., Graf, A., Greenshields, D., Takagi, H., Shinozaki, K., Terauchi, R., and Shirasu, K. and Ichimura, K.
Organizer
第57回日本植物生理学会年会
Place of Presentation
盛岡
Year and Date
2016-03-18
Related Report
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[Presentation] Toward elucidating complex regulation of Arabidopsis MEKK1 pathway in plant immunity signaling2015
Author(s)
Takagi, M., Hamano, K., Graf, A., Greenshields, D., Takagi, H., Shinozaki, K., Terauchi, R., Shirasu, K., Ichimura, K.
Organizer
第56回日本植物生理学会年会
Place of Presentation
東京農業大学 世田谷キャンパス
Year and Date
2015-03-16 – 2015-03-18
Related Report
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[Presentation] SMN1 is required for dwarf and cell death phenotypes of Arabidopsis mekk1 and mpk42015
Author(s)
Takagi, M., Hamano, K., Graf, A., Greenshields, D., Takagi, H., Shinozaki, K., Terauchi, R., Shirasu, K., Ichimura, K.
Organizer
第56回日本植物生理学会年会
Place of Presentation
東京農業大学 世田谷キャンパス
Year and Date
2015-03-16 – 2015-03-18
Related Report
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