Analysis of dwarf and constitutive defense responses activated by loss of specific Arabidopsis MAP kinase pathway involved in innate immunity signaling

• Project/Area Number: 25450060
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant protection science
• Research Institution: Kagawa University
• Principal Investigator: Ichimura Kazuya 香川大学, 農学部, 准教授 (70321726)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: サプレッサー変異体 / MAPキナーゼ経路 / 防御反応 / 矮性 / 抵抗性 / 変異体 / 抵抗性遺伝子 / 細胞死 / 活性酸素種蓄積 / 原因遺伝子同定

Outline of Final Research Achievements

Mitogen-activated protein kinase (MAPK) pathways have a pivotal role in innate immunity signaling in plants. Arabidopsis MEKK1, a MAPK kinase kinase, constitutes specific pathway with MKK1/MKK2 and MPK4. This pathway has an important role in regulation of defense gene expression in response to microbe associated molecular patterns recognition. However, loss of MEKK1 results in constitutive defense responses accompanying severe dwarfism and temperature-dependent cell death. To elucidate the complex mechanism underlying the mekk1 phenotypes, we performed genetic screening to isolate suppressor mutant of the dwarf and defense phenotypes. With the aid of the MutMap analysis, we identified the candidate of causal gene for the mutant. Genetic analysis by producing double mutant with mekk1 or mpk4, smn1 mutation suppressed the dwarf and defense phenotypes of these mutants. These results showed that SMN1 positively contributed to the phenotypes.

Research Products

• [Journal Article] SGT1 contributes to maintaining protein levels of MEK2DD to facilitate hypersensitive response-like cell death in Nicotiana benthamiana (2016)
  • Author(s): Kazuya Ichimura, Takehito Shinzato, Miki Edaki, Hirofumi Yoshioka, Ken Shirasu
  • Journal Title: Physiological and Molecular Plant Pathology Volume: 94 Pages: 47-52
  • DOI: 10.1016/j.pmpp.2016.04.001
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Mitogen-Activated Protein Kinase Kinase 3 Regulates Seed Dormancy in Barley (2016)
  • Author(s): Shingo Nakamura, Mohammad Pourkheirandish, Hiromi Morishige, Yuta Kubo, Masako Nakamura, Kazuya Ichimura, Shigemi Seo, Hiroyuki Kanamori, Jianzhong Wu, Tsuyu Ando, Goetz Hensel, Mohammad Sameri, Nils Stein, Kazuhiro Sato, Takashi Matsumoto, Masahiro Yano, Takao Komatsuda
  • Journal Title: Current Biology Volume: 26 Issue: 6 Pages: 775-781
  • DOI: 10.1016/j.cub.2016.01.024
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] 植物免疫応答の分子機構解明に向けた青枯病菌effectomicsアプローチ (2014)
  • Author(s): 市村和也,田口義人，佐藤幹也，吉岡博文，田中信和，秋光和也
  • Journal Title: 植物細菌病談話会論文集 Volume: 26 Pages: 29-37
• [Journal Article] Phosphorylation of D-allose by hexokinase involved in regulation of OsABF1 expression for growth inhibition in Oryza sativa L. (2013)
  • Author(s): Fukumoto, T., Kano, A., Ohtani, K., Inoue, M., Yoshihara, A., Izumori, K., Tajima, S., Shigematsu, Y., Tanaka, K., Ohkouchi, T., Ishida, Y., Nishizawa, Y., Tada, Y., Ichimura, K., Gomi, K., Yoo, SD., Sheen, J., Akimitsu, K.
  • Journal Title: Planta Volume: 237 Issue: 5 Pages: 1379-1391
  • DOI: 10.1007/s00425-013-1853-9
  • Peer Reviewed
• [Journal Article] The rare sugar D-allose acts as a triggering molecule of rice defence via ROS generation. (2013)
  • Author(s): Kano, A., Fukumoto, T., Ohtani, K., Yoshihara, A., Ohara, T., Tajima, S., Izumori, K., Tanaka, K., Ohkouchi, T., Ishida, Y., Nishizawa, Y., Ichimura, K., Tada, Y., Gomi, K., and Akimitsu, K.
  • Journal Title: Journal of Experimental Botany Volume: 64 Issue: 16 Pages: 4939-4951
  • DOI: 10.1093/jxb/ert282
  • Peer Reviewed
• [Presentation] シロイヌナズナMEKK1経路欠損変異体における表現型へのR 遺伝子SMN1 の関与 (2016)
  • Author(s): 高木桃子，濵野康平，Alexander Graf，David Greenshields，高木宏樹，篠崎一雄，寺内良平，白須 賢，市村和也
  • Organizer: 平成28年度日本植物病理学会大会
  • Place of Presentation: 岡山
  • Year and Date: 2016-03-21
• [Presentation] Functional interplay between NLR protein SMN1 and MEKK1 pathway in Arabidopsis (2016)
  • Author(s): Takagi, M., Graf, A., Greenshields, D., Takagi, H., Shinozaki, K., Terauchi, R., Shirasu, K., Ichimura, K
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 盛岡
  • Year and Date: 2016-03-18
• [Presentation] Loss of function of SMN1 suppressed constitutive defense phenotypes in MEKK1 pathway mutants (2016)
  • Author(s): Hamano, K., Takagi, M., Graf, A., Greenshields, D., Takagi, H., Shinozaki, K., Terauchi, R., and Shirasu, K. and Ichimura, K.
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 盛岡
  • Year and Date: 2016-03-18
• [Presentation] シロイヌナズナmekk1 経路変異体表現型に関与するR 遺伝子SMN1の解析 (2015)
  • Author(s): 濵野康平，高木桃子，坂田勇，Alexander Graf，David Greenshields，高木宏樹，篠崎一雄，寺内良平，白須 賢，市村和也
  • Organizer: 平成27年度日本植物病理学会関西部会
  • Place of Presentation: 徳島
  • Year and Date: 2015-09-29
• [Presentation] シロイヌナズナmekk1 変異体表現型におけるR 遺伝子SMN1 の転写調節 (2015)
  • Author(s): 高木桃子，Alexander Graf，David Greenshields，高木宏樹，篠崎一雄，寺内良平，白須 賢，市村和也
  • Organizer: 平成27年度日本植物病理学会関西部会
  • Place of Presentation: 徳島
  • Year and Date: 2015-09-29
• [Presentation] シロイヌナズナMEKK1遺伝子の欠損により誘導される防御反応表現型に関する解析 (2015)
  • Author(s): 高木桃子，Alexander Graf1，David Greenshields，高木宏樹，篠崎一雄，寺内良平，白須 賢，市村和也
  • Organizer: 平成26年度日本植物病理学会関西部会
  • Place of Presentation: 富山大学五福キャンパス
  • Year and Date: 2015-09-27 – 2015-09-28
• [Presentation] シロイヌナズナmpk4 変異体が示す矮性および防御反応表現型におけるSMN1遺伝子の関与 (2015)
  • Author(s): 濵野康平，高木桃子，坂田勇，Alexander Graf，David Greenshields，高木宏樹，篠崎一雄，寺内良平，白須 賢，市村和也
  • Organizer: 平成26年度日本植物病理学会関西部会
  • Place of Presentation: 富山大学五福キャンパス
  • Year and Date: 2015-09-27 – 2015-09-28
• [Presentation] シロイヌナズナmpk4 変異体が示す矮性及び防御反応表現型におけるSMN1遺伝子の関与 (2015)
  • Author(s): 濵野康平，高木桃子，坂田勇，Alexander Graf，David Greenshields，高木宏樹，篠崎一雄，寺内良平，白須 賢，市村和也
  • Organizer: 日本植物病理学会平成27年度植物感染生理談話会
  • Place of Presentation: 松山
  • Year and Date: 2015-08-24
• [Presentation] シロイヌナズナMEKKl経路の欠損により誘導される防御反応表現型に関する解析 (2015)
  • Author(s): 高木桃子，Alexander Graf，David Greenshields，高木宏樹，篠崎一雄，寺内良平，白須 賢，市村和也
  • Organizer: 日本植物病理学会平成27年度植物感染生理談話会
  • Place of Presentation: 松山
  • Year and Date: 2015-08-24
• [Presentation] シロイヌナズナMEKK1 遺伝子の欠損により誘導される防御反応表現型に関する解析 (2015)
  • Author(s): 高木桃子，市村和也，Alexander Graf，David Greenshields，高木宏樹，篠崎一雄，寺内良平，白須 賢
  • Organizer: 平成26年度日本植物病理学会大会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2015-06-02 – 2015-06-04
• [Presentation] シロイヌナズナMEKK1経路の欠損により誘導される防御反応表現型に関する解析 (2015)
  • Author(s): 高木桃子・濵野康平・Alexander Graf・ David Greenshields・高木宏樹・篠崎一雄・寺内良平・ 白須 賢・市村和也
  • Organizer: 日本植物病理学会 創立100周年記念大会
  • Place of Presentation: 明治大学 駿河台キャンパス
  • Year and Date: 2015-03-28 – 2015-03-31
• [Presentation] Toward elucidating complex regulation of Arabidopsis MEKK1 pathway in plant immunity signaling (2015)
  • Author(s): Takagi, M., Hamano, K., Graf, A., Greenshields, D., Takagi, H., Shinozaki, K., Terauchi, R., Shirasu, K., Ichimura, K.
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学 世田谷キャンパス
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] SMN1 is required for dwarf and cell death phenotypes of Arabidopsis mekk1 and mpk4 (2015)
  • Author(s): Takagi, M., Hamano, K., Graf, A., Greenshields, D., Takagi, H., Shinozaki, K., Terauchi, R., Shirasu, K., Ichimura, K.
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学 世田谷キャンパス
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] Two U-box Ubiquitin Ligases Positively Contribute to MAMP-Responsive MAP Kinase Cascade in Arabidopsis (2014)
  • Author(s): Yuta Kubo, Kazuya Ichimura, Junpei Hio, Tsuyoshi Mizoguchi, Alexander Graf, Fuminori Takahashi, Kazuo Shinozaki, Ken Shirasu
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 富山大学五福キャンパス(富山県)
• [Presentation] Forward Genetic Approach Toward Elucidating Complex Regulation Of Arabidopsis MEKK1 In Plant Immunity Signaling (2014)
  • Author(s): Momoko Takagi, Kazuya Ichimura, Alexander Graf, David Greenshields, Hiroki Takagi, Kazuo Shinozaki, RyoheiTerauchi, Ken Shirasu
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 富山大学五福キャンパス(富山県)
• [Presentation] Identification of Novel Ralstonia solanacearum GMI1000 Effector Which Suppress HR-like Cell Death Induced by MEK2DD (2014)
  • Author(s): Mikiya Sato, Yoshito Taguchi, Hirofumi Yoshioka, Kazuya Akimitsu, Kazuya Ichimura
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 富山大学五福キャンパス(富山県)
• [Presentation] HR様細胞死を抑制する青枯病菌エフェクターの機能解析 (2013)
  • Author(s): 田口義人, 吉岡博文, 田中伸和, 秋光和也, 市村和也
  • Organizer: 日本植物病理学会関西部会
  • Place of Presentation: 岡山大学(岡山県)
• [Presentation] U-box型ユビキチンリガーゼPUB25, 26はシロイズナズナMAMP応答性のMEKK1カスケードを促進的に制御する (2013)
  • Author(s): 久保佑太，市村和也，樋尾隼平，溝口剛，AlexanderGraf，高橋史憲，篠崎一雄，白須賢
  • Organizer: 日本植物病理学会関西部会
  • Place of Presentation: 岡山大学(岡山県)
• [Presentation] シロイヌナズナMEKK1遺伝子の欠損により誘導される防御反応表現型に関する解析 (2013)
  • Author(s): 高木桃子，市村和也，Alexander Graf，David Greenshields，高木宏樹，篠崎一雄，寺内良平，白須賢
  • Organizer: 日本植物病理学会関西部会
  • Place of Presentation: 岡山大学(岡山県)
• [Presentation] 構成的活性化型MEK2DDによるHR様細胞死を抑制する青枯病菌エフェクターの選抜 (2013)
  • Author(s): 佐藤幹也，田口義人，吉岡博文，秋光和也，市村和也
  • Organizer: 日本植物病理学会関西部会
  • Place of Presentation: 岡山大学(岡山県)
• [Presentation] Functional analysis of Ralstonia solanacearum GMI1000 effector which suppresses HR like cell death induced by MEK2DD in Nicotiana benthamiana (2013)
  • Author(s): Taguchi, Y., Sato, M., Yoshioka, H., Tanaka, N., and Ichimura, K.
  • Organizer: Japanese Solanaceae Genomics Initiative (JSOL) 10th International Symposium on Solanaceae Genomics
  • Place of Presentation: 大阪府立大学中百舌鳥キャンパス(大阪府)
• [Remarks] 香川大学農学部市村研究室
  • URL: http://www.ag.kagawa-u.ac.jp/kichimura/
• [Remarks] 香川大学農学部市村研究室
  • URL: http://www.ag.kagawa-u.ac.jp/kichimura/