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Functional analysis of novel enzymes from Xanthomonas euvesicatoria for extensin and lectin

Research Project

Project/Area Number 25450061
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant protection science
Research InstitutionKagoshima University

Principal Investigator

Nakamura Masayuki  鹿児島大学, 農水産獣医学域農学系, 准教授 (90404475)

Co-Investigator(Renkei-kenkyūsha) FUJITA KIYOTAKA  鹿児島大学, 農水産獣医学域農学系, 准教授 (20381189)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
KeywordsXanthomonas / アラビノシダーゼ / エクステンシン / ナス科レクチン / 病原性 / エクテンシン / レクチン
Outline of Final Research Achievements

Recently three novel arabinosidases have been found in Bifidobacteria and the orthologs of the enzymes exist on the genome of the plant pathogenic bacterium, Xanthomonas euvesicatoria (Xcv). In this work, the three orthologs (XcvHypBA1, XcvHypBA2, XcvHypAA)derived from Xcv were analyzed to elucidate their functions. As a result, it is revealed that the three enzymes work together and have functions to degrade arabino-oligosaccharides on glycoproteins such as extensin and lectin that are thought to be involved in the plant immune system. The expression of the enzyme genes were highly induced in infected plants, suggesting that the three enzymes might play an important role in pathogenicity.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (3 results)

All 2016 2015 2013

All Presentation (3 results)

  • [Presentation] トマト斑点細菌病菌 (Xcv) UPB139株由来GH43ファミリーα-L-アラビノフラノシダーゼ (XcvHypAA) の機能解析2016

    • Author(s)
      本田 傑・古澤 慧・中村正幸・藤田清貴・岩井 久
    • Organizer
      日本植物病理学会大会
    • Place of Presentation
      岡山コンベンションセンター (岡山県,岡山市)
    • Year and Date
      2016-03-21
    • Related Report
      2015 Annual Research Report
  • [Presentation] Xanthomonas campestris pv.campestris(Xcc)およびX.campestris pv.vesicatoria(Xcv)由来アラビノビオシダーゼ遺伝子(HypBA2)の発現解析2015

    • Author(s)
      古澤 慧・中村正幸・藤田清貴・岩井 久
    • Organizer
      九州病害虫研究会
    • Place of Presentation
      KKRホテル (熊本県熊本市)
    • Year and Date
      2015-02-05 – 2015-02-06
    • Related Report
      2014 Research-status Report
  • [Presentation] トマト斑点細菌病菌UPB139株由来β-アラビノオリゴ糖鎖分解酵素群のクローニングと機能解析2013

    • Author(s)
      渕脇玉央・中村正幸・藤田清貴・岩井 久
    • Organizer
      日本植物病理学会
    • Place of Presentation
      KKRホテル熊本
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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