Functional analysis of novel enzymes from Xanthomonas euvesicatoria for extensin and lectin
| Project/Area Number |
25450061
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Plant protection science
|
|---|
| Research Institution | Kagoshima University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
FUJITA KIYOTAKA 鹿児島大学, 農水産獣医学域農学系, 准教授 (20381189)
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
|
|---|
| Keywords | Xanthomonas / アラビノシダーゼ / エクステンシン / ナス科レクチン / 病原性 / エクテンシン / レクチン |
|---|
| Outline of Final Research Achievements |
Recently three novel arabinosidases have been found in Bifidobacteria and the orthologs of the enzymes exist on the genome of the plant pathogenic bacterium, Xanthomonas euvesicatoria (Xcv). In this work, the three orthologs (XcvHypBA1, XcvHypBA2, XcvHypAA)derived from Xcv were analyzed to elucidate their functions. As a result, it is revealed that the three enzymes work together and have functions to degrade arabino-oligosaccharides on glycoproteins such as extensin and lectin that are thought to be involved in the plant immune system. The expression of the enzyme genes were highly induced in infected plants, suggesting that the three enzymes might play an important role in pathogenicity.
|
Report
(4 results)
Research Products
(3 results)
