Molecular analysis and characterization of the broad-spectrum cyst nematode resistant tomato
| Project/Area Number |
25450063
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Plant protection science
|
|---|
| Research Institution | National Agriculture and Food Research Organization |
|---|
Principal Investigator |
UEHARA Taketo 国立研究開発法人農業・食品産業技術総合研究機構, 中央農業研究センター虫・鳥獣害研究領域, 上級研究員 (30355458)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
MASUTA Chikara 北海道大学, 大学院農学研究院, 教授 (60281854)
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Keywords | トマト / Globodera / HeroA / タバコシストセンチュウ / 抵抗性遺伝子 / 抵抗性 / ジャガイモシストセンチュウ / 線虫 |
|---|
| Outline of Final Research Achievements |
①We confirmed that Potato Cyst Nematode (PCN)-resistant tomato cultivars in Japan contained the HeroA gene, whereas susceptible cultivars did not. ②PCN-resistant tomato cultivars are identical to Tabacco Cyst Nematode (TCN)-resistant tomato cultivars. ③When segregated progenies from the selfing of resistant cultivar (Doctor-K) were inoculated with PCN, the progenies segregated into 20 resistant and five susceptible plants, fitting the 3 : 1 ratio expected for single-gene inheritance (χ2=0.61, P=0.56). ④Microarray analyses were performed to investigate the transcriptome profiles in the incompatible and compatible interactions at 3 and 7 days postinoculation (dpi).The PR1(P4,P6) transcript increased to exceptionally high level at 3 dpi in the cyst nematode-infected resistant plants compared to the uninoculated controls. ⑤We elucidated the effective of cyst nematode-resistant tomato cultivars in reducing the population of TCN in greenhouse traials.
|
Report
(4 results)
Research Products
(5 results)
