| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
①We confirmed that Potato Cyst Nematode (PCN)-resistant tomato cultivars in Japan contained the HeroA gene, whereas susceptible cultivars did not. ②PCN-resistant tomato cultivars are identical to Tabacco Cyst Nematode (TCN)-resistant tomato cultivars. ③When segregated progenies from the selfing of resistant cultivar (Doctor-K) were inoculated with PCN, the progenies segregated into 20 resistant and five susceptible plants, fitting the 3 : 1 ratio expected for single-gene inheritance (χ2=0.61, P=0.56). ④Microarray analyses were performed to investigate the transcriptome profiles in the incompatible and compatible interactions at 3 and 7 days postinoculation (dpi).The PR1(P4,P6) transcript increased to exceptionally high level at 3 dpi in the cyst nematode-infected resistant plants compared to the uninoculated controls. ⑤We elucidated the effective of cyst nematode-resistant tomato cultivars in reducing the population of TCN in greenhouse traials.
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