Characterization of dual-specigicity protein kinase BSR1
Project/Area Number |
25450066
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Plant protection science
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Research Institution | National Agriculture and Food Research Organization (2016) National Institute of Agrobiological Sciences (2013-2015) |
Principal Investigator |
Shoji Sugano 国立研究開発法人農業・食品産業技術総合研究機構, 生物機能利用研究部門 植物・微生物機能利用研究領域, 上級研究員 (60242111)
|
Research Collaborator |
MORI Masaki 国立研究開発法人農業・食品産業技術総合研究機構, 生物機能利用研究部門植物・微生物機能利用研究領域, ユニット長 (50192779)
|
Project Period (FY) |
2013-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | タンパク質リン酸化 / 複合病害抵抗性 / イネ / 病害抵抗性 |
Outline of Final Research Achievements |
Rice BSR1 is a dual-specificity protein kinase whose overexpression confers disease resistance against multiple pathogens. In this study, we have identified autophosphorylated tyrosine residues in BSR1. We have also mutated each tyrosine residues and examined the effects of mutations on kinase activity and tyrosine phosphorylation of BSR1. By overexpressing kinase-dead or tyrosine-replaced mutant bsr1, we have shown that kinase activity is essential for disease resistance and that tyrosine phosphorylation is important for full activity of BSR1 in disease resistance. We have also shown that SA signaling is dispensable for disease resistance conferred by BSR1.
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Report
(5 results)
Research Products
(9 results)
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[Journal Article] Rice OsVAMP714, a membrane-trafficking protein localized to the chloroplast and vacuolar membrane, is involved in resistance to rice blast disease2016
Author(s)
Shoji Sugano, Nagao Hayashi, Yasushi Kawagoe, Susumu Mochizuki, Haruhiko Inoue, Masaki Mori, Yoko Nishizawa, Chang-Jie Jiang, Minami Matsui, Hiroshi Takatsuji
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Journal Title
Plant Molecular Biology
Volume: 91
Issue: 1-2
Pages: 81-95
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Development of disease-resistant rice by optimized expression of WRKY452015
Author(s)
Goto S, Sasakura-Shimoda F, Suetsugu M, Selvaraj M.G, Hayashi N, Yamazaki M, Ishitani M, Shimono M, Sugano S, Matsushita A, Tanabata T, Takatsuji H
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Journal Title
Plant Biotechnology Journal
Volume: ahead of print
Issue: 6
Pages: 753-765
DOI
Related Report
Peer Reviewed
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[Journal Article] WRKY76 is a rice transcriptional repressor playing opposite roles in blast disease resistance and cold stress tolerance2013
Author(s)
Yokotani N, Sato Y, Tanabe S, Chujo T, Shimizu T, Okada K, Yamane H, Shimono M, Sugano S, Takatsuji H, Kaku H, Minami E, Nishizawa Y
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Journal Title
J. of Experimental Botany
Volume: 64
Pages: 5085-5097
Related Report
Peer Reviewed
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[Journal Article] Genome-wide identification of WRKY45-regulated genes that mediate benzothiadiazole-induced defense responses in rice2013
Author(s)
Nakayama A, Fukushima S, Goto S, Matsushita A, Shimono M, Sugano S, Jiang C-J, Akagi A, Yamazaki M, Inoue H, Takatsuji H
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Journal Title
BMC Plant Biology
Volume: 13
Pages: 150-150
Related Report
Peer Reviewed
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