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Clarification of the factors that involve plant reoviral infection and multiplication in insect vector cell monolayer.

Research Project

Project/Area Number 25450073
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant protection science
Research InstitutionNational Agriculture and Food Research Organization (2016)
National Institute of Agrobiological Sciences (2013-2015)

Principal Investigator

Ichiki Tamaki  国立研究開発法人農業・食品産業技術総合研究機構, 遺伝資源センター, 上級研究員 (70355501)

Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsイネ科植物ウイルス / 昆虫培養細胞 / 細胞機能阻害剤 / RNA干渉 / イネ萎縮ウイルス / 培養細胞 / Hsp70 / 昆虫の培養細胞 / 宿主遺伝子
Outline of Final Research Achievements

Rice dwarf virus and rice gall dwarf virus, which are members of the family Reoviridae are transmitted by insect vectors (Nephotettix cincticeps) and infect rice plants. To control these diseases, preventing disease cycle of insect-plant is important. To understand the factors that play important role of insect host and virus interactions, some protein inhibitors were treated to vector cell monolayer (VCM) derived N. cincticeps. After the treatment, RDV or RGDV were inoculated to VCM. The application of 50μM Quercetin resulted in a drastic inhibition of RDV and RGDV infection/multiplication. This result suggested Hsp70 of VCM have important functions for RDV and RGDV infection/multiplication. The RNAi experiments were performed using Hsp70/Hsc70 VCM genes from EST analysis. We found RNAi Hsp70 genes resulted that reduction of multiplication RDV in VCM. These results showed that the host factor, Hsp70 has important role for infection/multiplication of rice reoviruses in insect cells.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (8 results)

All 2015 2014 2013

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (7 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Detection and diagnosis of rice-infecting viruses2013

    • Author(s)
      Tamaki Uehara-Ichiki, Takuya Shiba, Keiichiro Matsukura,Takanori Ueno, Masahiro Hirae, Takahide Sasaya
    • Journal Title

      Frontiers in Microbiology

      Volume: 4 (289) Pages: 1-7

    • DOI

      10.3389/fmicb.2013.00289

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] THe high-resolution X-ray crystal structure of the capping enzyme from Rice Dwarf Virus2015

    • Author(s)
      Nakamichi Y, Higashiura A, Narita H, Ichiki T,Hagiwara K, Omura T, Nakagawa A
    • Organizer
      The 13th conference of the Asian Crystallographic Association
    • Place of Presentation
      Kolkata India
    • Year and Date
      2015-12-05
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] バイロプラズマ構成蛋白質Pns12の位相差クライオ電子顕微鏡解析とX線結晶構造解析による構造学的研究2015

    • Author(s)
      東浦彰史,宮崎直幸,中道優介,山本旭麻,梶浦直起,北尾雅博,秋田総理,清水巧,一木珠樹,大村敏博,村田和義,中川敦史
    • Organizer
      SSSEM研究部会&生理研究会合同ワークショップ
    • Place of Presentation
      岡崎コンファレンスセンター
    • Year and Date
      2015-11-18
    • Related Report
      2015 Research-status Report
  • [Presentation] 高圧凍結法を用いたイネ萎縮ウイルスの高分解能X線結晶構造解析への取り組み2015

    • Author(s)
      東浦彰史,中道優介,太田和敬,宮崎直幸,一木珠樹,大村敏博,中川敦史
    • Organizer
      平成27年度日本結晶学会年会
    • Place of Presentation
      大阪府立大学 中百舌鳥キャンパス
    • Year and Date
      2015-10-17
    • Related Report
      2015 Research-status Report
  • [Presentation] イネ萎縮ウイルス由来キャップ化酵素P5のX線結晶構造解析2015

    • Author(s)
      中道優介,東浦彰史,成田宏隆,一木珠樹,萩原恭二,大村敏博,中川敦史
    • Organizer
      平成27年度日本結晶学会年会
    • Place of Presentation
      大阪府立大学 中百舌鳥キャンパス
    • Year and Date
      2015-10-17
    • Related Report
      2015 Research-status Report
  • [Presentation] X線結晶構造解析に基づく変異体解析によるイネ萎縮ウイルス由来viroplasmタンパク質Pns12の多量体形成機構の解明2015

    • Author(s)
      山本旭麻,中道優介,東浦彰史,梶浦直起,北尾雅博,宮崎直幸,秋田総理,清水巧,一木珠樹,大村敏博,中川敦史
    • Organizer
      第38会日本分子生物学会年会、第88回日本生化学会大会合同大会(BMB2015)
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2015-10-17
    • Related Report
      2015 Research-status Report
  • [Presentation] イネ萎縮ウイルスの外殻構築機構の解明2015

    • Author(s)
      中道優介,東浦彰史,宮崎直幸,成田宏隆,清水巧,一木珠樹,大村敏博,中川敦史
    • Organizer
      第38会日本分子生物学会年会、第88回日本生化学会大会合同大会(BMB2015)
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2015-10-17
    • Related Report
      2015 Research-status Report
  • [Presentation] 熱ショックタンパク質(Hsp70)はファイトレオウイルスの媒介昆虫細胞内での増殖に関与する2014

    • Author(s)
      5)一木(植原)珠樹、大橋美保、上野敬規、宮崎直幸、中川敦史
    • Organizer
      日本ウイルス学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-11-11
    • Related Report
      2014 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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