| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Azorhizobium caulinodans is a microsymbiont of a tropical legume Sesbania rostrata. This bacterium possesses reb gene that are related to production of R-bodies, which are host-killing factors. The expression of reb genes is usually repressed in the bacterial cells inhabiting the plant host cells. In this study, we found that the expression of the reb genes was controlled by at least two environmental factors, 2-oxoglutareta and temperature. When A. caulinodans was supplied with high amount of 2-oxoglutarate (more than several mM) at lower temperatures than optimum growth temperatures (about 37°C), high expression of the reb genes was induced. Furthermore, we found that the nuclei of host plant cells were collapsed when A. caulinodans cells produced R-bodies in the host plant cells.
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