Characterization of a novel symbiosis pathway in legume-rhizobium symbiosis

• Project/Area Number: 25450082
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: OKAZAKI SHIN 東京農工大学, (連合)農学研究科(研究院), 助教 (40379285)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: マメ科植物 / 根粒菌 / 共生 / III型分泌機構 / エフェクター / 植物免疫 / マメ科 / 根粒形成 / ダイズ

Outline of Final Research Achievements

We identified a novel gene (orf5208) in a soybean symbiont Bradyrhizobium elkanii which affected root nodule symbiosis with soybean. The orf5208 encoded a putative secreted protein and was shown to inhibit nodule formation on Rj4 soybean while it promoted nodule formation on non-Rj4 soybean. Furthermore, orf5208 was shown to be essential for the nodule formation on soybean mutant that had a defect in early symbiosis signaling. Taken together, orf5208 promotes nodule formation via the novel pathway that bypasses the early symbiosis signaling.

Research Products

• [Int'l Joint Research] CIRAD(フランス)
• [Journal Article] Rhizobium–legume symbiosis in the absence of Nod factors: two possible scenarios with or without the T3SS (2016)
  • Author(s): Okazaki S., Tittabutr P., Teulet A., Thouin J., Fardoux J., Chaintreuil, C. Gully D., Arrighi JF., Furuta N., Miwa H., Yasuda M., Nouwen N., Teaumroong N and Giraud E
  • Journal Title: The ISME Journal Volume: 10 Issue: 1 Pages: 64-74
  • DOI: 10.1038/ismej.2015.103
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Identification of Bradyrhizobium elkanii Genes Involved in Incompatibility with Soybean Plants Carrying the Rj4 Allele (2015)
  • Author(s): Faruque OM., Miwa H., Yasuda M., Fujii Y., Kaneko T., Sato S. and Okazaki S.
  • Journal Title: Applied Environental Microbiology Volume: 81 Issue: 19 Pages: 6710-6717
  • DOI: 10.1128/aem.01942-15
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Genome analysis of a novel Bradyrhizobium sp. DOA9 carrying a symbiotic plasmid. (2015)
  • Author(s): Okazaki S, Noisangiam R, Okubo T, Kaneko T, Oshima K, Hattori M, Teamtisong K, Songwattana P, Tittabutr P, Boonkerd N, Saeki K, Sato S, Uchiumi T, Minamisawa K, *Teaumroong N.
  • Journal Title: PLOS ONE Volume: 10(2) Issue: 2 Pages: 1-18
  • DOI: 10.1371/journal.pone.0117392
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Microbiological and soil analysis of the growth-promotion effect of hairy vetch on velvet bean (2015)
  • Author(s): Okazaki S, Yuan K, Iizuka M, Yasuda M, Oikawa Y, Kimura S.D. and Fujii Y
  • Journal Title: Acta Advanced in Agricultural Sciences Volume: 3 Pages: 1-10
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Microbiological and soil analysis of the growth-promotion effect of hairy vetch on velvet bean (2015)
  • Author(s): Okazaki S, Yuan K, Iizuka M, Yasuda M, Oikawa Y, Bellingrath-Kimura S.D. and Fujii Y.
  • Journal Title: Advances in Applied Agricultural Science Volume: 3 Pages: 1-10
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Divergent <i>Nod</i>-Containing <i>Bradyrhizobium</i> sp. DOA9 with a Megaplasmid and its Host Range (2014)
  • Author(s): Teamtisong K, Songwattana P, Noisa-ngiam R, Piromyou P, Boonkerd N, Tittabutr P, Minamisawa K, Nantakit A, Okazaki S, Abe M, *Uchiumi T, Teaumroong N
  • Journal Title: Microbes and Environments Volume: 29 Issue: 4 Pages: 370-376
  • DOI: 10.1264/jsme2.ME14065
  • NAID: 130004693790
  • ISSN: 1342-6311, 1347-4405
  • Peer Reviewed / Open Access
• [Journal Article] ダイズシスト線虫応答性遺伝子を利用したシスト線虫抵抗性ダイズ育種の試み (2014)
  • Author(s): 岡崎伸
  • Journal Title: 大豆たん白質研究 Volume: 16 Pages: 173-176
• [Journal Article] Commonalities and Differences among Symbiosis Islands of Three <i>Mesorhizobium loti</i> Strains (2013)
  • Author(s): Kasai-Maita, H, Hirakawa H, Nakamura Y, Kaneko, T, Miki K, Maruya J, Okazaki S, Tabata S, Saeki K and Sato S
  • Journal Title: Microbes and Environments Volume: 28 Issue: 2 Pages: 275-278
  • DOI: 10.1264/jsme2.ME12201
  • NAID: 10031158633
  • ISSN: 1342-6311, 1347-4405
  • Peer Reviewed
• [Journal Article] Hijacking of leguminous nodulation signaling by the rhizobial type III secretion system (2013)
  • Author(s): Okazaki S, Kaneko T, Sato S and Saeki K
  • Journal Title: Proc. Natl. Acad. Sci. U. S. A. Volume: 110 Issue: 42 Pages: 17131-17136
  • DOI: 10.1073/pnas.1302360110
  • Peer Reviewed
• [Presentation] The Nod factor-independent and T3SS-dependent nodulation in Rhizobium&#8211;legume symbiosis (2015)
  • Author(s): Okazaki S, Teulet A, Yasuda M, Furuta N, Miwa H, Giraud E.
  • Organizer: 第30回微生物生態学会土浦大会
  • Place of Presentation: 土浦亀城プラザ
  • Year and Date: 2015-10-22
• [Presentation] Molecular genetic analysis of Bradyrhizobium elkanii mutants with altered symbiotic compatibility with Vigna radiata (2015)
  • Author(s): Hien Nguyen Phuoc, Faruque Omar, Hiroki Miwa and Shin Okazaki
  • Organizer: Japan-Taiwan-Korea (JTK) International Symposium - JSME 2015
  • Place of Presentation: 土浦亀城プラザ
  • Year and Date: 2015-10-22
  • Int'l Joint Research
• [Presentation] Bradyrhizobium elkanii の3型分泌系によるミヤコグサ根粒形成の制御 (2015)
  • Author(s): Hiroki MIWA, Michiko YASUDA, Sachiko MASUDA, Takakazu KANEKO, Shusei SATO, Shin OKAZAKI
  • Organizer: 日本微生物生態学会第30回大会
  • Place of Presentation: 土浦亀城プラザ
  • Year and Date: 2015-10-22
• [Presentation] Characterization of transposon mutants of Bradyrhizobium elkanii USDA61 that formed effective nodule on Rj4 soybean cv. BARC2 (2015)
  • Author(s): Faruque Muhammad Omar, Michiko Yasuda, Hiroki Miwa, Yoshiharu Fujii , Takakazu Kaneko, Shusei Sato, Shin Okazaki
  • Organizer: Japan-Taiwan-Korea (JTK) International Symposium - JSME 2015
  • Place of Presentation: 土浦亀城プラザ
  • Year and Date: 2015-10-22
  • Int'l Joint Research
• [Presentation] Rj4遺伝子保有ダイズとの非親和性を決定する根粒菌3型エフェクター (2015)
  • Author(s): 増田 幸子, 三輪 大樹, Faruque Muhammad Omar, 安田 美智子, 岡崎 伸
  • Organizer: 日本微生物生態学会第30回大会
  • Place of Presentation: 土浦亀城プラザ
  • Year and Date: 2015-10-22
• [Presentation] Rhizobial type III secretion system activates leguminous nodulation signaling in the absence of Nod factor (2015)
  • Author(s): Okazaki S.
  • Organizer: 19th International Congress on Nitrogen Fixation Conference
  • Place of Presentation: California, USA
  • Year and Date: 2015-10-04
  • Int'l Joint Research / Invited
• [Presentation] Isolation and characterization of Bradyrhizobium elkanii mutants with altered nodulation compatibility with Vigna radiata (2015)
  • Author(s): Hien Nguyen Phuoc, Faruque Omar, Hiroki Miwa and Shin Okazaki
  • Organizer: 第25回植物微生物研究交流会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2015-09-14
• [Presentation] 根粒菌 3 型分泌系による根粒形成の制御機構 (2015)
  • Author(s): 三輪大樹, Faruque Omar, 増田幸子, 安田美智子, 金子貴一, 佐藤修正, 岡崎伸
  • Organizer: 第25回植物微生物研究交流会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2015-09-14
• [Presentation] Rhizobial type III secretion system controls host-dependent nodulation on soybean (2014)
  • Author(s): Okazaki S
  • Organizer: The 3rd Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation
  • Place of Presentation: Chengdu, China
  • Year and Date: 2014-10-28 – 2014-10-31
• [Presentation] Putative type III effector proteins of Bradyrhizobium elkanii USDA61 cause Incompatibility with Rj4 soybean (2014)
  • Author(s): Omar FM, Yasuda M, Miwa H, Okazaki S
  • Organizer: The 3rd Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation
  • Place of Presentation: Chengdu, China
  • Year and Date: 2014-10-28 – 2014-10-31
• [Presentation] Roles of the type III secretion system of Bradyrhizobium elkanii in the symbiotic incompatibility with Rj4 soybean (2014)
  • Author(s): Yasuda M, Miwa H, Okazaki S
  • Organizer: The 3rd Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation
  • Place of Presentation: Chengdu, China
  • Year and Date: 2014-10-28 – 2014-10-31
• [Presentation] Profiling of the novel symbiotic pathway of Bradyrhizobium sp. ORS278 by RNA-Seq analysis (2014)
  • Author(s): Furuta N, Yasuda M, Okazaki S
  • Organizer: The 3rd Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation
  • Place of Presentation: Chengdu, China
  • Year and Date: 2014-10-28 – 2014-10-31
• [Presentation] Genetic diversity of rhizobia associated with hairy vetch in Japan (2014)
  • Author(s): Yuan K, Iizuka M, Miwa H, Yasuda M, Kimura S.D., Fujii Y, Okazaki S
  • Organizer: The 3rd Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation
  • Place of Presentation: Chengdu, China
  • Year and Date: 2014-10-28 – 2014-10-31
• [Presentation] Genetic diversity of rhizobia associated with hairy vetch in Japan (2014)
  • Author(s): YUAN K, IIZUKA M, YASUDA M, KIMURA SD, FUJII Y, OKAZAKI S
  • Organizer: 日本土壌肥料学会2014年度東京大会
  • Place of Presentation: 東京農工大学
  • Year and Date: 2014-09-09 – 2014-09-11
• [Presentation] ヘアリーベッチによるハッショウマメ生育促進の要因解明 (2014)
  • Author(s): 飯塚真貴, 岡崎伸, 元坤, 藤井義晴
  • Organizer: 日本土壌肥料学会2014年度東京大会
  • Place of Presentation: 東京農工大学
  • Year and Date: 2014-09-09 – 2014-09-11
• [Presentation] Molecular Analysis of Nodulation Incompatibility between Rj4 soybean and Bradyrhizobium elkanii (2014)
  • Author(s): Omar M.F. and Okazaki S
  • Organizer: 日本土壌肥料学会2014年度東京大会
  • Place of Presentation: 東京農工大学
  • Year and Date: 2014-09-09 – 2014-09-11
• [Presentation] Hijacking of leguminous nodulation signaling by the rhizobial type III secretion system (2014)
  • Author(s): Okazaki S
  • Organizer: The 11th European Nitrogen Fixation Conference
  • Place of Presentation: Tenerife, Spain
  • Year and Date: 2014-09-07 – 2014-09-10
  • Invited
• [Presentation] III型分泌機構による根粒菌の新規感染機構とその応用 (2013)
  • Author(s): 岡崎伸
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 名古屋大学
• [Presentation] Molecular analysis of nodulation incompatibility between Rj4 soybean and Bradyrhizobium elkanii USDA61 (2013)
  • Author(s): Faruque Muhammad Omar, Yoshiharu Fujii, Shin Okazaki
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 名古屋大学
• [Presentation] Hijacking of leguminous nodulation signaling by the rhizobial type III secretion system (2013)
  • Author(s): Okazaki S, Kaneko T, Sato S, Saeki K.
  • Organizer: The18th International Congress on Nitrogen Fixation
  • Place of Presentation: 宮崎県シーガイアワールドコンベンションセンター サミット
• [Presentation] Isolation and characterization of Tn5 mutants of Bradyrhizobium elkanii with altered compatibility with Rj4-soybean and Vigna radiata (2013)
  • Author(s): Faruque Muhammad Omar, Michiko Yasuda, Shin Okazaki
  • Organizer: The18th International Congress on Nitrogen Fixation
  • Place of Presentation: 宮崎県シーガイアワールドコンベンションセンター サミット
• [Presentation] The type III section system of Bradyrhizobium elkanii mediates symbiotic incompatibility with Rj4 soybean (2013)
  • Author(s): Michiko YASUDA, Shin OKAZAKI
  • Organizer: The18th International Congress on Nitrogen Fixation
  • Place of Presentation: 宮崎県シーガイアワールドコンベンションセンター サミット
• [Book] The Lotus japonicus Genome (2014)
  • Author(s): Nakagawa T, Okazaki S, Shibuya N
  • Total Pages: 267
  • Publisher: Springer-Verlag Berlin Heidelberg
• [Remarks] 東京農工大学 農学府 国際環境農学専攻 植物微生物研究室（岡崎研究室）
  • URL: http://web.tuat.ac.jp/~okazaki/
• [Remarks] 東京農工大学 農学府 国際環境農学専攻 植物微生物研究室
  • URL: http://www.tuat.ac.jp/~okazaki/