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Characterization of a novel symbiosis pathway in legume-rhizobium symbiosis

Research Project

Project/Area Number 25450082
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

OKAZAKI SHIN  東京農工大学, (連合)農学研究科(研究院), 助教 (40379285)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywordsマメ科植物 / 根粒菌 / 共生 / III型分泌機構 / エフェクター / 植物免疫 / マメ科 / 根粒形成 / ダイズ
Outline of Final Research Achievements

We identified a novel gene (orf5208) in a soybean symbiont Bradyrhizobium elkanii which affected root nodule symbiosis with soybean. The orf5208 encoded a putative secreted protein and was shown to inhibit nodule formation on Rj4 soybean while it promoted nodule formation on non-Rj4 soybean. Furthermore, orf5208 was shown to be essential for the nodule formation on soybean mutant that had a defect in early symbiosis signaling. Taken together, orf5208 promotes nodule formation via the novel pathway that bypasses the early symbiosis signaling.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (35 results)

All 2016 2015 2014 2013 Other

All Int'l Joint Research (1 results) Journal Article (9 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 8 results,  Open Access: 6 results,  Acknowledgement Compliant: 2 results) Presentation (22 results) (of which Int'l Joint Research: 3 results,  Invited: 2 results) Book (1 results) Remarks (2 results)

  • [Int'l Joint Research] CIRAD(フランス)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Rhizobium–legume symbiosis in the absence of Nod factors: two possible scenarios with or without the T3SS2016

    • Author(s)
      Okazaki S., Tittabutr P., Teulet A., Thouin J., Fardoux J., Chaintreuil, C. Gully D., Arrighi JF., Furuta N., Miwa H., Yasuda M., Nouwen N., Teaumroong N and Giraud E
    • Journal Title

      The ISME Journal

      Volume: 10 Issue: 1 Pages: 64-74

    • DOI

      10.1038/ismej.2015.103

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Identification of Bradyrhizobium elkanii Genes Involved in Incompatibility with Soybean Plants Carrying the Rj4 Allele2015

    • Author(s)
      Faruque OM., Miwa H., Yasuda M., Fujii Y., Kaneko T., Sato S. and Okazaki S.
    • Journal Title

      Applied Environental Microbiology

      Volume: 81 Issue: 19 Pages: 6710-6717

    • DOI

      10.1128/aem.01942-15

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Genome analysis of a novel Bradyrhizobium sp. DOA9 carrying a symbiotic plasmid.2015

    • Author(s)
      Okazaki S, Noisangiam R, Okubo T, Kaneko T, Oshima K, Hattori M, Teamtisong K, Songwattana P, Tittabutr P, Boonkerd N, Saeki K, Sato S, Uchiumi T, Minamisawa K, *Teaumroong N.
    • Journal Title

      PLOS ONE

      Volume: 10(2) Issue: 2 Pages: 1-18

    • DOI

      10.1371/journal.pone.0117392

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Microbiological and soil analysis of the growth-promotion effect of hairy vetch on velvet bean2015

    • Author(s)
      Okazaki S, Yuan K, Iizuka M, Yasuda M, Oikawa Y, Kimura S.D. and Fujii Y
    • Journal Title

      Acta Advanced in Agricultural Sciences

      Volume: 3 Pages: 1-10

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Microbiological and soil analysis of the growth-promotion effect of hairy vetch on velvet bean2015

    • Author(s)
      Okazaki S, Yuan K, Iizuka M, Yasuda M, Oikawa Y, Bellingrath-Kimura S.D. and Fujii Y.
    • Journal Title

      Advances in Applied Agricultural Science

      Volume: 3 Pages: 1-10

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Divergent <i>Nod</i>-Containing <i>Bradyrhizobium</i> sp. DOA9 with a Megaplasmid and its Host Range2014

    • Author(s)
      Teamtisong K, Songwattana P, Noisa-ngiam R, Piromyou P, Boonkerd N, Tittabutr P, Minamisawa K, Nantakit A, Okazaki S, Abe M, *Uchiumi T, Teaumroong N
    • Journal Title

      Microbes and Environments

      Volume: 29 Issue: 4 Pages: 370-376

    • DOI

      10.1264/jsme2.ME14065

    • NAID

      130004693790

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] ダイズシスト線虫応答性遺伝子を利用したシスト線虫抵抗性ダイズ育種の試み2014

    • Author(s)
      岡崎伸
    • Journal Title

      大豆たん白質研究

      Volume: 16 Pages: 173-176

    • Related Report
      2014 Research-status Report
  • [Journal Article] Commonalities and Differences among Symbiosis Islands of Three <i>Mesorhizobium loti</i> Strains2013

    • Author(s)
      Kasai-Maita, H, Hirakawa H, Nakamura Y, Kaneko, T, Miki K, Maruya J, Okazaki S, Tabata S, Saeki K and Sato S
    • Journal Title

      Microbes and Environments

      Volume: 28 Issue: 2 Pages: 275-278

    • DOI

      10.1264/jsme2.ME12201

    • NAID

      10031158633

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Hijacking of leguminous nodulation signaling by the rhizobial type III secretion system2013

    • Author(s)
      Okazaki S, Kaneko T, Sato S and Saeki K
    • Journal Title

      Proc. Natl. Acad. Sci. U. S. A.

      Volume: 110 Issue: 42 Pages: 17131-17136

    • DOI

      10.1073/pnas.1302360110

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] The Nod factor-independent and T3SS-dependent nodulation in Rhizobium&#8211;legume symbiosis2015

    • Author(s)
      Okazaki S, Teulet A, Yasuda M, Furuta N, Miwa H, Giraud E.
    • Organizer
      第30回微生物生態学会土浦大会
    • Place of Presentation
      土浦亀城プラザ
    • Year and Date
      2015-10-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] Molecular genetic analysis of Bradyrhizobium elkanii mutants with altered symbiotic compatibility with Vigna radiata2015

    • Author(s)
      Hien Nguyen Phuoc, Faruque Omar, Hiroki Miwa and Shin Okazaki
    • Organizer
      Japan-Taiwan-Korea (JTK) International Symposium - JSME 2015
    • Place of Presentation
      土浦亀城プラザ
    • Year and Date
      2015-10-22
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Bradyrhizobium elkanii の3型分泌系によるミヤコグサ根粒形成の制御2015

    • Author(s)
      Hiroki MIWA, Michiko YASUDA, Sachiko MASUDA, Takakazu KANEKO, Shusei SATO, Shin OKAZAKI
    • Organizer
      日本微生物生態学会第30回大会
    • Place of Presentation
      土浦亀城プラザ
    • Year and Date
      2015-10-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] Characterization of transposon mutants of Bradyrhizobium elkanii USDA61 that formed effective nodule on Rj4 soybean cv. BARC22015

    • Author(s)
      Faruque Muhammad Omar, Michiko Yasuda, Hiroki Miwa, Yoshiharu Fujii , Takakazu Kaneko, Shusei Sato, Shin Okazaki
    • Organizer
      Japan-Taiwan-Korea (JTK) International Symposium - JSME 2015
    • Place of Presentation
      土浦亀城プラザ
    • Year and Date
      2015-10-22
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Rj4遺伝子保有ダイズとの非親和性を決定する根粒菌3型エフェクター2015

    • Author(s)
      増田 幸子, 三輪 大樹, Faruque Muhammad Omar, 安田 美智子, 岡崎 伸
    • Organizer
      日本微生物生態学会第30回大会
    • Place of Presentation
      土浦亀城プラザ
    • Year and Date
      2015-10-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] Rhizobial type III secretion system activates leguminous nodulation signaling in the absence of Nod factor2015

    • Author(s)
      Okazaki S.
    • Organizer
      19th International Congress on Nitrogen Fixation Conference
    • Place of Presentation
      California, USA
    • Year and Date
      2015-10-04
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Isolation and characterization of Bradyrhizobium elkanii mutants with altered nodulation compatibility with Vigna radiata2015

    • Author(s)
      Hien Nguyen Phuoc, Faruque Omar, Hiroki Miwa and Shin Okazaki
    • Organizer
      第25回植物微生物研究交流会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2015-09-14
    • Related Report
      2015 Annual Research Report
  • [Presentation] 根粒菌 3 型分泌系による根粒形成の制御機構2015

    • Author(s)
      三輪大樹, Faruque Omar, 増田幸子, 安田美智子, 金子貴一, 佐藤修正, 岡崎伸
    • Organizer
      第25回植物微生物研究交流会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2015-09-14
    • Related Report
      2015 Annual Research Report
  • [Presentation] Rhizobial type III secretion system controls host-dependent nodulation on soybean2014

    • Author(s)
      Okazaki S
    • Organizer
      The 3rd Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation
    • Place of Presentation
      Chengdu, China
    • Year and Date
      2014-10-28 – 2014-10-31
    • Related Report
      2014 Research-status Report
  • [Presentation] Putative type III effector proteins of Bradyrhizobium elkanii USDA61 cause Incompatibility with Rj4 soybean2014

    • Author(s)
      Omar FM, Yasuda M, Miwa H, Okazaki S
    • Organizer
      The 3rd Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation
    • Place of Presentation
      Chengdu, China
    • Year and Date
      2014-10-28 – 2014-10-31
    • Related Report
      2014 Research-status Report
  • [Presentation] Roles of the type III secretion system of Bradyrhizobium elkanii in the symbiotic incompatibility with Rj4 soybean2014

    • Author(s)
      Yasuda M, Miwa H, Okazaki S
    • Organizer
      The 3rd Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation
    • Place of Presentation
      Chengdu, China
    • Year and Date
      2014-10-28 – 2014-10-31
    • Related Report
      2014 Research-status Report
  • [Presentation] Profiling of the novel symbiotic pathway of Bradyrhizobium sp. ORS278 by RNA-Seq analysis2014

    • Author(s)
      Furuta N, Yasuda M, Okazaki S
    • Organizer
      The 3rd Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation
    • Place of Presentation
      Chengdu, China
    • Year and Date
      2014-10-28 – 2014-10-31
    • Related Report
      2014 Research-status Report
  • [Presentation] Genetic diversity of rhizobia associated with hairy vetch in Japan2014

    • Author(s)
      Yuan K, Iizuka M, Miwa H, Yasuda M, Kimura S.D., Fujii Y, Okazaki S
    • Organizer
      The 3rd Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation
    • Place of Presentation
      Chengdu, China
    • Year and Date
      2014-10-28 – 2014-10-31
    • Related Report
      2014 Research-status Report
  • [Presentation] Genetic diversity of rhizobia associated with hairy vetch in Japan2014

    • Author(s)
      YUAN K, IIZUKA M, YASUDA M, KIMURA SD, FUJII Y, OKAZAKI S
    • Organizer
      日本土壌肥料学会2014年度東京大会
    • Place of Presentation
      東京農工大学
    • Year and Date
      2014-09-09 – 2014-09-11
    • Related Report
      2014 Research-status Report
  • [Presentation] ヘアリーベッチによるハッショウマメ生育促進の要因解明2014

    • Author(s)
      飯塚真貴, 岡崎伸, 元坤, 藤井義晴
    • Organizer
      日本土壌肥料学会2014年度東京大会
    • Place of Presentation
      東京農工大学
    • Year and Date
      2014-09-09 – 2014-09-11
    • Related Report
      2014 Research-status Report
  • [Presentation] Molecular Analysis of Nodulation Incompatibility between Rj4 soybean and Bradyrhizobium elkanii2014

    • Author(s)
      Omar M.F. and Okazaki S
    • Organizer
      日本土壌肥料学会2014年度東京大会
    • Place of Presentation
      東京農工大学
    • Year and Date
      2014-09-09 – 2014-09-11
    • Related Report
      2014 Research-status Report
  • [Presentation] Hijacking of leguminous nodulation signaling by the rhizobial type III secretion system2014

    • Author(s)
      Okazaki S
    • Organizer
      The 11th European Nitrogen Fixation Conference
    • Place of Presentation
      Tenerife, Spain
    • Year and Date
      2014-09-07 – 2014-09-10
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] III型分泌機構による根粒菌の新規感染機構とその応用2013

    • Author(s)
      岡崎伸
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      名古屋大学
    • Related Report
      2013 Research-status Report
  • [Presentation] Molecular analysis of nodulation incompatibility between Rj4 soybean and Bradyrhizobium elkanii USDA612013

    • Author(s)
      Faruque Muhammad Omar, Yoshiharu Fujii, Shin Okazaki
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      名古屋大学
    • Related Report
      2013 Research-status Report
  • [Presentation] Hijacking of leguminous nodulation signaling by the rhizobial type III secretion system2013

    • Author(s)
      Okazaki S, Kaneko T, Sato S, Saeki K.
    • Organizer
      The18th International Congress on Nitrogen Fixation
    • Place of Presentation
      宮崎県シーガイアワールドコンベンションセンター サミット
    • Related Report
      2013 Research-status Report
  • [Presentation] Isolation and characterization of Tn5 mutants of Bradyrhizobium elkanii with altered compatibility with Rj4-soybean and Vigna radiata2013

    • Author(s)
      Faruque Muhammad Omar, Michiko Yasuda, Shin Okazaki
    • Organizer
      The18th International Congress on Nitrogen Fixation
    • Place of Presentation
      宮崎県シーガイアワールドコンベンションセンター サミット
    • Related Report
      2013 Research-status Report
  • [Presentation] The type III section system of Bradyrhizobium elkanii mediates symbiotic incompatibility with Rj4 soybean2013

    • Author(s)
      Michiko YASUDA, Shin OKAZAKI
    • Organizer
      The18th International Congress on Nitrogen Fixation
    • Place of Presentation
      宮崎県シーガイアワールドコンベンションセンター サミット
    • Related Report
      2013 Research-status Report
  • [Book] The Lotus japonicus Genome2014

    • Author(s)
      Nakagawa T, Okazaki S, Shibuya N
    • Total Pages
      267
    • Publisher
      Springer-Verlag Berlin Heidelberg
    • Related Report
      2014 Research-status Report
  • [Remarks] 東京農工大学 農学府 国際環境農学専攻 植物微生物研究室(岡崎研究室)

    • URL

      http://web.tuat.ac.jp/~okazaki/

    • Related Report
      2015 Annual Research Report
  • [Remarks] 東京農工大学 農学府 国際環境農学専攻 植物微生物研究室

    • URL

      http://www.tuat.ac.jp/~okazaki/

    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

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