Elucidation of the symbiosis mechanism to improve the nitrogen fixation in legume

• Project/Area Number: 25450084
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: Kagawa University
• Principal Investigator: Agarie (Nomura) Mika 香川大学, 農学部, 教授 (50294749)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 共生窒素固定 / ミヤコグサ / 根粒菌 / 老化 / マイクロアレー / マメ科植物 / SNARE / 突然変異体

Outline of Final Research Achievements

Iron is an essential micronutrient for the legume rhizobium symbiosis and is accumulated abundantly in nodule. Iron homeostasis needs to be strictly controlled to avoid toxicity. Homeostasis of iron in plants is achieved through processes requiring proteins and small organic molecules. Among the molecules, ferritins are a class of iron storage proteins. During nodule senescence, total iron concentration in nodule was decreased, whereas expression of ferritin, Ljfer1, was induced in Lotus japonicus. We have identified signal transduction pathway leading to the increase of Ljfer1 in nodule. The Ljfer1 promoter of L. japonicus is conserved Iron-Dependent Regulatory Sequence (IDRS). The expression of Ljfer1 was induced by application of iron or sodium nitroprusside. Moreover, application of iron in nodule increased the nitric oxide (NO). These data indicate that iron-induced NO leads to increase the expression of Ljfer1 in the senescence of L. japonicus nodule.

Research Products

• [Journal Article] Peribacteroid solution of soybean root nodules partly induces genomic loci for differentiation into bacteroids of free-living Bradyrhizobium japonicum cells (2015)
  • Author(s): 2.Ohtsu, N, Ichida, S, Yamaya, H, Ohwada, T, Itakura, M, Hara, Y, Mitsui, H, Kaneko, T, Tejima, K, Tabata, S, Saeki, K, Omori, H, Hayashi, M, Maekawa, T, Murooka, Y, Tajima, S, Simomura, K, Agarie-Nomura, M, Uchiumi, T, Suzuki, A, Shimoda, Y, Abe, M, Minamisawa, K, Arima, Y, Yokoyama, T.
  • Journal Title: Soil Science and Plant Nutrition Volume: 61 Pages: 461-470
  • NAID: 110010031454
  • Peer Reviewed
• [Journal Article] Transcriptomic profiles of nodule senescence in <i>Lotus japonicus</i> and <i>Mesorhizobium loti</i> symbiosis (2014)
  • Author(s): 2.Chungopast, S., Hirakawa, H., Sato, S., Handa, Y., Saito, K., Kawaguchi, M., Tajima, S., Nomura, M.
  • Journal Title: Plant Biotechnology Volume: 31 Issue: 4 Pages: 345-349
  • DOI: 10.5511/plantbiotechnology.14.1021a
  • NAID: 130004713023
  • ISSN: 1342-4580, 1347-6114
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Glutamine synthetase I-deficiency in Mesorhizobium loti differentially affects nodule development and activity in Lotus japonicus (2013)
  • Author(s): Chungopast, S., Thapanapongworakul, P., Matsuura, H., Dao, T.V., Asahi, T., Tada, K., Tajima, S., and Nomura, M
  • Journal Title: Journal of Plant Physiology Volume: 171 Issue: 5 Pages: 104-108
  • DOI: 10.1016/j.jplph.2013.10.015
  • Peer Reviewed
• [Journal Article] IDENTIFICATION OF ENDOPHYTIC BACTERIA ASSOCIATED WITH N2 FIXATION AND INDOLE ACETIC ACID SYNTHESIS AS GROWTH PROMOTERS IN CURCUMA ALISMATIFOLIA GAGNEP (2013)
  • Author(s): Thepsukhon, A., Choonluchanon, S., Tajima, S., Nomura, M., and Ruamrungsri, S.
  • Journal Title: Journal of Plant Nutrition Volume: 36 Issue: 9 Pages: 1424-1438
  • DOI: 10.1080/01904167.2013.793712
  • Peer Reviewed
• [Presentation] Expression of ferritin in Lotus japonicus nodule (2015)
  • Author(s): Chungopast Sirinapa, Mallika Duangkhet, Jian Feng Ma, Shigeyuki Tajima, Mika Nomura
  • Organizer: 日本土壌肥料学会 関西支部講演会
  • Place of Presentation: 愛媛
  • Year and Date: 2015-12-11
• [Presentation] Effect of MYB transcription factor during nodule development in Lotus japonicus (2015)
  • Author(s): Supriadi, Rahayu Widyastuti, Dwi Andreas Santosa, Shigeyuki Tajima, Mika Nomura
  • Organizer: 日本土壌肥料学会 関西支部講演会
  • Place of Presentation: 愛媛
  • Year and Date: 2015-12-11
• [Presentation] 根粒老化時に発現するフェリチンタンパク質はNOによって誘導する (2015)
  • Author(s): 野村美加, Sirinapa Chungopast, 田島茂行
  • Organizer: 植物微生物研究会
  • Place of Presentation: つくば
  • Year and Date: 2015-09-14
• [Presentation] ミヤコグサQa-SNARE遺伝子Syn1の根粒形成および種子形成への関与 (2014)
  • Author(s): 十川蒼、 小澤拓也、 山崎大樹、 三好貴紘、 京正晴、 田島茂行、 野村美加
  • Organizer: 日本土壌肥料学会関西支部講演会
  • Place of Presentation: サンポートホール高松
  • Year and Date: 2014-12-11 – 2014-12-12
• [Presentation] Isolation and characterization of Lotus japonicus EMS41 mutant that can be expected yield increase (2014)
  • Author(s): Nomura, M, Yasunaga, M, Minoura, Y, Miyoshi, T, Kawaguchi, M, Tajima, S
  • Organizer: Phytogene Symposium VII
  • Place of Presentation: サンポートホール高松
  • Year and Date: 2014-09-30
• [Presentation] Effect of Syn1 Qa-SNARE for nodule and seed formation in L. japonicus (2014)
  • Author(s): Sogawa, A, Ozawa, T, Yamasaki, H, Miyoshi, T, Tajima, S and Nomura, M
  • Organizer: Phytogene Symposium VII
  • Place of Presentation: サンポートホール高松
  • Year and Date: 2014-09-30
• [Presentation] Legume-rhizobium symbiosis requires the Ljvamp72-1 in an exocytotic pathway of Lotus japonicus. (2014)
  • Author(s): M.Nomura, A. Sogawa, S.chungopast, K.Yokota, M. Hayashi S.Tajima
  • Organizer: 11th European Nitrogen Fixation Conference,
  • Place of Presentation: Tenerife, Spain
  • Year and Date: 2014-09-08 – 2014-09-10
• [Presentation] Deficient glutamine synthetase mutant induces early nodule senescence in Lotus japonicus, (2013)
  • Author(s): Chungopast, S., Matsuura, H., Thapanapongworakul, N., Tajima, S., and Nomura, M.
  • Organizer: 18th International Congress on Nitrogen Fixation
  • Place of Presentation: 宮崎
• [Presentation] SNARE, Syn1 gene, affects symbiotic nitrogen fixation in Lotus japonicus (2013)
  • Author(s): Sogawa, A., Yamasaki, H., Miyoshi, T., Nomura, M., and Tajima, S.
  • Organizer: 18th International Congress on Nitrogen Fixation
  • Place of Presentation: 宮崎
• [Presentation] Legume-rhizobium symbiosis requires the Ljvamp72-1 in an exocytotic pathway of Lotus japonicus (2013)
  • Author(s): Nomura, M.
  • Organizer: ファイトジーンの可能性と未来VI
  • Place of Presentation: 香川
• [Presentation] Lotus japonicus Syn1 SNARE is required for the nodule and seed formations, (2013)
  • Author(s): Sogawa, A., Yamasaki, H., Miyoshi, T., Tajima, S., Nomura, M.
  • Organizer: ファイトジーンの可能性と未来VI
  • Place of Presentation: 香川
• [Presentation] Screening of Lotus Japonicus EMS mutant with high nitrogen fixation activity (2013)
  • Author(s): Minoura, Y., Miyoshi, T., Abe, S., Tajima, S., Nomura, M.
  • Organizer: ファイトジーンの可能性と未来VI
  • Place of Presentation: 香川
• [Presentation] 根粒菌感染のための輸送小胞に関わる遺伝子の分子進化 (2013)
  • Author(s): 野村美加, Chungopast Sirinapa, 十川蒼, 横田圭祐,林誠, 田島茂行
  • Organizer: 日本土壌肥料学会 関西支部講演会
  • Place of Presentation: 山口