Development of self-cloning of symbiotic genes of rhizobium

• Project/Area Number: 25450085
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: Kagawa University
• Principal Investigator: Shigeyuki Tajima 香川大学, 農学部, 教授 (50116894)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 遺伝子解析 / 共生窒素固定 / 根粒代謝 / マメ科 / 根粒菌 / ミヤコグサ / セルフクローニング / 代謝 / 老化 / 根粒老化 / マメ科植物 / 遺伝子応答 / RNAseq / マイクロアレー / トランスクリプトーム解析 / 植物微生物相互作用 / glutamine synthetase I

Outline of Final Research Achievements

We surveyed proteome and RNA seq analysis of nodule tissue formation and during aging using a legume plant (Lotus japonicum) and a rhizobium (Mesorhizobium loti). Cluster analysis of these gene expression profiles suggested the tissue and age specific metabolic genes of these organisms. The function of the tissue specific genes were surveyed using transgenic Lotus japonicum/meosrhizobium system. GS1 (glutamine synthetase I) gene of the M. loti was especially curious because the mutant showed strong effect to stumulate aging of the nodules. We studied the function of the gene and developed the technique to perform self-cloning of the gene in rhizobium.

Research Products

• [Journal Article] Peribacteroid solution of soybean root nodules partly induces genomic loci for differentiation into bacteroids of free-living Bradyrhizobium japonicum cells (2015)
  • Author(s): Ohtsu, N, Ichida, S, Yamaya, H, Ohwada, T, Itakura, M, Hara, Y, Mitsui, H, Kaneko, T, Tejima, K, Tabata, S, Saeki, K, Omori, H, Hayashi, M, Maekawa, T, Murooka, Y, Tajima, S, Simomura, K, Agarie-Nomura, M, Uchiumi, T, Suzuki, A, Shimoda, Y, Abe, M, Minamisawa, K, Arima, Y, Yokoyama, T.
  • Journal Title: Soil Science and Plant Nutrition Volume: 61 Pages: 461-470
  • NAID: 110010031454
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Peribacteroid solution of soybean root nodules partly induces genomic loci for differentiation into bacteroids of free-living Bradyrhizobium japonicum cells (2015)
  • Author(s): Ohkama-Ohtsu N., Ichida S., Yamaya H., Ohwada T., Itakura M., Hara Y., Mitsui H., Kaneko T., Tabata S., Tejima K., Saeki K., Omori H., Hayashi M., Maekawa M., Murooka Y., Tajima S., Simomura S., Nomura M., Uchiumi T., Suzuki A., Shimoda Y., Abe M., Minamisawa K., Arima Y., Yokoyama T.（責任著者）
  • Journal Title: Soil Sci. Plant Nutr. Volume: 60 Issue: 3 Pages: 461-470
  • DOI: 10.1080/00380768.2014.994470
  • NAID: 110010031454
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Transcriptomic profiles of nodule senescence in <i>Lotus japonicus</i> and <i>Mesorhizobium loti</i> symbiosis (2014)
  • Author(s): 2.Chungopast, S., Hirakawa, H., Sato, S., Handa, Y., Saito, K., Kawaguchi, M., Tajima, S., Nomura, M.
  • Journal Title: Plant Biotechnology Volume: 31 Issue: 4 Pages: 345-349
  • DOI: 10.5511/plantbiotechnology.14.1021a
  • NAID: 130004713023
  • ISSN: 1342-4580, 1347-6114
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] IDENTIFICATION OF ENDOPHYTIC BACTERIA ASSOCIATED WITH N2 FIXATION AND INDOLE ACETIC ACID SYNTHESIS AS GROWTH PROMOTERS IN CURCUMA ALISMATIFOLIA GAGNEP (2013)
  • Author(s): Thepsukhon, A., Choonluchanon, S., Tajima, S., Nomura, M., and Ruamrungsri, S.
  • Journal Title: Journal of Plant Nutrition Volume: 36 Issue: 9 Pages: 1424-1438
  • DOI: 10.1080/01904167.2013.793712
  • Peer Reviewed
• [Journal Article] Glutamine synthetase I-deficiency in Mesorhizobium loti differentially affects nodule development and activity in Lotus japonicus (2013)
  • Author(s): Chungopast, S., Thapanapongworakul, P., Matsuura, H., Dao, T.V., Asahi, T., Tada, K., Tajima, S., and Nomura, M
  • Journal Title: Journal of Plant Physiology Volume: 171 Issue: 5 Pages: 104-108
  • DOI: 10.1016/j.jplph.2013.10.015
  • Peer Reviewed