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Processes of degradation of mono-, di- and poly-saccharides in soil

Research Project

Project/Area Number 25450086
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionShizuoka Institute of Science and Technology

Principal Investigator

Saito Akihiro  静岡理工科大学, 理工学部, 准教授 (50375614)

Co-Investigator(Kenkyū-buntansha) SAMESHIMA REIKO (SAITO REIKO)  静岡大学, 農学部, 准教授 (00377722)
DOHI HIROFUMI  千葉大学, 大学院園芸学研究科, 准教授 (10345928)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords糖質 / 微生物 / 酵素 / キチン / アセチルグルコサミン / ジアセチルキトビオース / アンモニア態窒素 / 土壌微生物 / 土壌酵素 / キチナーゼ / アセチルグルコサミニダーゼ / 土壌改良資材 / 放線菌 / キトサン
Outline of Final Research Achievements

Many of soil bacteria assimilating polysaccharides hydrolyzes the polysacchrides into corresponding disaccharides as major products by extracellular enzymes. To clarify the importance of the disaccharides in soil ecosystems, we investigated the effects of mono- and di-saccharides on the number of soil microorganisms, the activities of polysaccharide degrading enzymes, the amounts of inorganic nitrogens, and the structures of soil microbiome. The results clearly demonstrated that the alteration patterns of ammonium contents, the bacterial and fungal counts, and the soil enzymatic activities differs according to the chain length of the saccharides (i.e. monosaccharide, disaccharide, and polysaccharides).

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (22 results)

All 2015 2014 2013 Other

All Journal Article (6 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Acknowledgement Compliant: 1 results) Presentation (16 results) (of which Invited: 2 results)

  • [Journal Article] Chitosan degradation and associated changes in bacterial community structures in two contrasting soils2015

    • Author(s)
      Ami Sawaguchi, Shouta Ono, Masaru Oomura, Kaori Inami, Yuta Kumeta, Kazuma Honda, Reiko Sameshima-Saito, Kazunori Sakamoto, Akikazu Ando, Akihiro Saito
    • Journal Title

      Soil Science and Plant Nutrition

      Volume: 61 Issue: 3 Pages: 471-480

    • DOI

      10.1080/00380768.2014.1003965

    • NAID

      110010031455

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Uptake of chitosan-derived D-glucosamine oligosaccharides in Streptomyces coelicolor A3(2)2015

    • Author(s)
      Viens P, Dubeau MP, Kimura A, Desaki Y, Shinya T, Shibuya N, Saito A,Brzezinski R.
    • Journal Title

      FEMS Microbiol Lett

      Volume: 362 Issue: 9 Pages: 9-9

    • DOI

      10.1093/femsle/fnv048

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Bis(b-lactosyl)-[60]fullerene as novel class of glycolipids useful for detection and decontamination of biological toxhins in Ricinus communis family2014

    • Author(s)
      Dohi H, Kanazawa T, Saito A, Sato K, Uzawa H, Seto Y, Nishida Y
    • Journal Title

      Beilstein Journal of Organic Chemistry

      Volume: 10 Pages: 1504-1512

    • DOI

      10.3762/bjoc.10.155

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] キチン分解の分子生物学と土壌微生物学2014

    • Author(s)
      齋藤明広
    • Journal Title

      土と微生物

      Volume: 68 Pages: 79-80

    • Related Report
      2014 Research-status Report
  • [Journal Article] Characterization of antifungal activity of the GH-46 subclass III chitosasanase from Bacillus circulans MH-K12013

    • Author(s)
      Tomita M, Kikuchi A, Kobayashi M, Yamaguchi S, Ifuku S, Yamashoji S, Ando A, Saito A
    • Journal Title

      Antonie van Leeuwenhoek Journal of Microbiology

      Volume: 104 Issue: 5 Pages: 737-748

    • DOI

      10.1007/s10482-013-9982-5

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Organ- and stage-specific expression of the lectin gene in tomato2013

    • Author(s)
      Kouzai Y, Saito A
    • Journal Title

      静岡理工科大学紀要

      Volume: 26 Pages: 27-33

    • NAID

      120006554236

    • Related Report
      2013 Research-status Report
  • [Presentation] Uptake of a chitin-degradation product via the constitutive N-acetylglucosamine/N,N’-diacetylchitobiose binding protein NgcESCO of an ABC transporter in Streptomyces coelicolor A3(2)2015

    • Author(s)
      Akihiro Saito, Chibaru Iinuma, Takayuki Ohnuma, Tomonori Shinya, Yusuke Kimura, Yuzusa Aoki, Liu Feng, Yoshitake Desaki, Naoto Shibuya, Tamo Fukamizo, Akikazu Ando, Takeshi Fujii, Kiyotaka Miyashita
    • Organizer
      日本微生物生態学会
    • Place of Presentation
      土浦亀城プラザ
    • Year and Date
      2015-10-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] キチン添加が畑土壌のキチン分解関連酵素と微生物に及ぼす影響 ‐キチン添加90日後までの影響-2015

    • Author(s)
      井浪かおり、粂田佑太、鮫島(齋藤)玲子、齋藤明広
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      東京農工大学小金井キャンパス
    • Year and Date
      2015-09-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] Streptomyces属放線菌のキチン分解物トランスポーター2015

    • Author(s)
      齋藤明広
    • Organizer
      日本キチン・キトサン学会
    • Place of Presentation
      東海大学熊本キャンパス
    • Year and Date
      2015-08-21
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 畑土壌でのキチン分解とそれに伴う微生物群集構造と酵素活性の変化2015

    • Author(s)
      井浪かおり、小野翔太、粂田佑太、榑松愛理、大塚恵巳、鮫島(齋藤)玲子、齋藤明広
    • Organizer
      日本キチン・キトサン学会
    • Place of Presentation
      東海大学熊本キャンパス
    • Year and Date
      2015-08-21
    • Related Report
      2015 Annual Research Report
  • [Presentation] MH-K1キトサナーゼの構造と反応機構2015

    • Author(s)
      鈴木道彦、齋藤明広、小林麻理子、横山知史、大宮祥子、栗剣、杉田慶、三木邦夫、齋藤純一、安藤昭一
    • Organizer
      日本キチン・キトサン学会
    • Place of Presentation
      東海大学熊本キャンパス
    • Year and Date
      2015-08-21
    • Related Report
      2015 Annual Research Report
  • [Presentation] ナタマメ根粒菌の分類と収穫量への影響2015

    • Author(s)
      増田航、畠中雄佑、齋藤明広
    • Organizer
      日本土壌微生物学会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2015-05-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] ナタマメ根粒菌から分離された細菌株のDNA-DNA交雑法とMALDI-TOF/MSによる類別2014

    • Author(s)
      増田航、畠中雄佑、岡崎伸、上村桂一、齋藤明広
    • Organizer
      環境微生物系学会合同大会2014
    • Place of Presentation
      アクトシティ浜松
    • Year and Date
      2014-10-23
    • Related Report
      2014 Research-status Report
  • [Presentation] 土壌環境下における放線菌キチナーゼ遺伝子群と抗生物質生産関連遺伝子群の発現誘導2014

    • Author(s)
      飯野藤樹、ナザリベナム、齋藤明広、王勇、藤井毅
    • Organizer
      環境微生物系学会合同大会2014
    • Place of Presentation
      アクトシティ浜松
    • Year and Date
      2014-10-23
    • Related Report
      2014 Research-status Report
  • [Presentation] キチン添加が畑土壌の微生物とキチン分解関連酵素に及ぼす影響2014

    • Author(s)
      小野翔太、粂田佑太、本田一馬、石丸梢、山﨑誠志、鮫島(齋藤)玲子、齋藤明広
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      東京農工大学
    • Year and Date
      2014-09-10
    • Related Report
      2014 Research-status Report
  • [Presentation] 「蟹漬」からのキチン分解微生物の分離2014

    • Author(s)
      岩堀昂平、山本広也、内田泰、関清彦、光富勝、齋藤明広
    • Organizer
      日本キチン・キトサン学会
    • Place of Presentation
      順天堂大学
    • Year and Date
      2014-08-07
    • Related Report
      2014 Research-status Report
  • [Presentation] ナタマメ根粒からの微生物の分離と同定

    • Author(s)
      増田航、齋藤明広
    • Organizer
      日本土壌肥料学会2013年度大会
    • Place of Presentation
      東京農工大学府中キャンパス
    • Related Report
      2013 Research-status Report
  • [Presentation] 放線菌ABC輸送体の基質特異性解明に向けたキトビオース型二糖類の合成

    • Author(s)
      須山奈月、藤田実加子、田澤健太、中島政哉、齋藤明広、西田芳弘、土肥博史
    • Organizer
      日本糖質学会
    • Place of Presentation
      大阪国際交流センター
    • Related Report
      2013 Research-status Report
  • [Presentation] キトサン検出用タンパク質プローブの開発と糸状菌の細胞壁構造の解析への利用

    • Author(s)
      菊地彩美、安藤昭一、齋藤明広
    • Organizer
      第44回 中部化学関係学協会支部連合秋季大会 (浜松)
    • Place of Presentation
      静岡大学工学部
    • Related Report
      2013 Research-status Report
  • [Presentation] Characterization of antifungal activity of the GH-46 subclass III chitosanase from Bacillus circulans MH-K1

    • Author(s)
      Akihiro Saito, Masayo Tomita, Ayami Kikuchi, Masashi Yamaguchi, Shinsuke Ifuku, Akikazu Ando
    • Organizer
      10th Asia-Pasific Chitin Chitosan Symposium
    • Place of Presentation
      米子コンベンションセンター
    • Related Report
      2013 Research-status Report
  • [Presentation] 土壌でのキトサンの分解と微生物群集構造の変化

    • Author(s)
      小野翔太,本田一馬,大村大,澤口亜美,坂本一憲,鮫島(齋藤)玲子,安藤昭一,齋藤明広
    • Organizer
      日本微生物生態学会
    • Place of Presentation
      鹿児島大学
    • Related Report
      2013 Research-status Report
  • [Presentation] キトサン分解酵素の抗菌作用とキトサン検出用プローブの開発

    • Author(s)
      齋藤明広
    • Organizer
      第25回静岡ライフサイエンスシンポジウム
    • Place of Presentation
      静岡理工科大学
    • Related Report
      2013 Research-status Report
    • Invited

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Published: 2014-07-25   Modified: 2019-07-29  

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