Development of a diagnostic method for boron deficiency in plants using borate cross-linking ratio of pectin in cell walls as an index

• Project/Area Number: 25450087
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: MATSUNAGA Toshiro 国立研究開発法人農業・食品産業技術総合研究機構, 中央農業総合研究センター土壌肥料研究領域, 上席研究員 (20355647)
• Co-Investigator(Kenkyū-buntansha): CHISHAKI Naoya 鹿児島大学, 農学部, 准教授 (60244266)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: ホウ素欠乏診断 / ホウ酸架橋率 / 植物細胞壁 / ペクチン / ラムノガラクツロナンⅡ / 細胞壁 / ソラマメ黒変障害

Outline of Final Research Achievements

The function of boron in plants is nowadays well established to be the stabilization of cell walls by borate cross-linking of wall pectin. We attempted to develop a diagnostic method for boron deficiency in plants using the borate cross-linking ratio of pectin as an index. Recently, blackening disorder of broad bean pods caused by boron deficiency was observed in farmers’ fields in Kagoshima prefecture, Japan. We used the affected broad bean plants as samples to demonstrate the usefulness of the method of diagnosis for boron deficiency occurred in fields. Then we applied the diagnostic method to several crops on which boron deficiency was suspected from the symptom, and finally proposed diagnostic criteria for boron deficiency.

Research Products

• [Journal Article] 細胞壁ペクチンのホウ酸架橋率は植物のホウ素欠乏の有用な診断指標となる (2015)
  • Author(s): 松永俊朗
  • Journal Title: 化学と生物 Volume: 53 Pages: 812-814
  • Acknowledgement Compliant
• [Journal Article] 細胞壁ペクチンのホウ酸架橋率を指標とする農家圃場ソラマメのホウ素欠乏診断 (2014)
  • Author(s): 松永俊朗，樗木直也
  • Journal Title: 日本土壌肥料学雑誌 Volume: 85 Pages: 453-457
  • NAID: 110009909579
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Abnormal Glycosphingolipid Mannosylation Triggers Salicylic Acid-Mediated Responses in Arabidopsis (2013)
  • Author(s): Mortimer JC, Yu X, Albrecht S, Sicilia F, Huichalaf M, Ampuero D, Michaelson LV, Murphy AM, Matsunaga T, Kurz S, Stephens E, Baldwin TC, Ishii T, Napier JA, Weber AP, Handford MG, Dupree P.
  • Journal Title: Plant Cell Volume: 25 Issue: 5 Pages: 1881-1894
  • DOI: 10.1105/tpc.113.111500
  • Peer Reviewed
• [Journal Article] Roles of BOR2, an efflux-type boron transporter, in crosslinking of rhamnogalacturonan II and root elongation under boron limitation in Arabidopsis thaliana. (2013)
  • Author(s): Miwa, K., Wakuta, S., Takada, S., Ide, K., Takano, J., Naito, S., Omori, H., Matsunaga, T., Fujiwara, T
  • Journal Title: Plant Physiology Volume: 163 Issue: 4 Pages: 1699-1709
  • DOI: 10.1104/pp.113.225995
  • Peer Reviewed
• [Presentation] 細胞壁ペクチンのホウ酸架橋率を指標とする作物のホウ素欠乏診断法 (2015)
  • Author(s): 松永 俊朗、樗木 直也
  • Organizer: 日本土壌肥料学会2015年度京都大会
  • Place of Presentation: 京都大学吉田南総合館（京都府京都市）
  • Year and Date: 2015-09-09
• [Presentation] ホウ素ラムノガラクツロナンⅡ複合体の機能に関する研究 －特異的構成糖KDOの合成制御株の解析－ (2014)
  • Author(s): 青木亮輔，小林優，間藤徹，岩元明敏，松永俊朗
  • Organizer: 日本土壌肥料学会2014年度東京大会
  • Place of Presentation: 東京農工大学（東京都小金井市）
  • Year and Date: 2014-09-09 – 2014-09-11
• [Presentation] 鹿児島県の農家ほ場においてソラマメのホウ素吸収に影響を及ぼす土壌要因（その2） －作土の深さと土壌ホウ素含量の影響－ (2014)
  • Author(s): 樗木直也，山田美帆，山倉愛，赤木功
  • Organizer: 日本土壌肥料学会2014年度東京大会
  • Place of Presentation: 東京農工大学（東京都小金井市）
  • Year and Date: 2014-09-09 – 2014-09-11
• [Presentation] ソラマメのホウ素欠乏症とカルシウム欠乏症
  • Author(s): 樗木直也
  • Organizer: 平成25年度関東東海土壌肥料技術連絡協議会春季研究会
  • Place of Presentation: 全農コープビル（東京都千代田区）
  • Invited
• [Remarks] 松永俊朗の仕事のサイト
  • URL: http://cse.naro.affrc.go.jp/tmatunag/
• [Remarks] 松永俊朗の仕事のサイト
  • URL: http://cse.naro.affrc.go.jp/tmatunag/