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Exploration of bifidobacterial genes involved in the intestinal survival through a dynamics analysis of random transposon mutants of Bifidobacterium longum

Research Project

Project/Area Number 25450090
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied microbiology
Research InstitutionHokkaido University

Principal Investigator

FUKIYA Satoru  北海道大学, (連合)農学研究科(研究院), 講師 (10370157)

Co-Investigator(Kenkyū-buntansha) OGURA Yoshitoshi  九州大学, 大学院医学研究院, 准教授 (40363585)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywordsトランスポゾン変異導入 / 転移因子 / Bifidobacterium longum / INSeq法 / 腸内生存 / ビフィズス菌 / プロモーター / ゲノム解析 / 定着因子 / ランダム変異導入
Outline of Final Research Achievements

Bifidobacteria, which exert health-promoting effects, are one of the beneficial members of the human intestinal microbiota. Molecular mechanisms of their intestinal survival are still unclear due to the insufficient development of gene-mutagenesis systems in this genus. This study aimed to identify bifidobacterial genes that contribute to the intestinal survival using combined analysis of a transposon mutagenesis and next-generation sequencing.
In this study, the complete genomic sequence of the mutagenesis-host strain, Bifidobacterium longum 105-A, was determined. Two different transposon mutagenesis systems were developed using transposable elements ISBlo11, derived from B. longum, and Himar1C9. This progress will pave the way to clarify the molecular mechanisms of bifidobacterial survival in the intestine.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (15 results)

All 2016 2015 2014 2013

All Journal Article (4 results) (of which Peer Reviewed: 3 results,  Acknowledgement Compliant: 4 results,  Open Access: 2 results) Presentation (11 results) (of which Invited: 1 results)

  • [Journal Article] ビフィズス菌における実用的な遺伝子変異導入系の開発2016

    • Author(s)
      吹谷 智
    • Journal Title

      生物工学会誌

      Volume: 94 Pages: 110-116

    • Related Report
      2015 Annual Research Report
    • Acknowledgement Compliant
  • [Journal Article] Isolation and transposition properties of ISBlo11, an active insertion sequence belonging to the IS3 family, from Bifidobacterium longum 105-A2015

    • Author(s)
      Sakanaka M, Fukiya S, Kobayashi R, Abe A, Hirayama Y, Kano Y, Yokota A
    • Journal Title

      FEMS Microbiol. Lett.

      Volume: 362 Issue: 7

    • DOI

      10.1093/femsle/fnv032

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Functional analysis of bifidobacterial promoters in Bifidobacterium longum and Escherichia coli using the α-galactosidase gene as a reporter2014

    • Author(s)
      Sakanaka, M., Tamai, S., Hirayama, Y., Onodera, A., Koguchi, H., Kano, Y., Yokota, A., Fukiya, S.
    • Journal Title

      J. Biosci. Bioeng.

      Volume: 118 Issue: 5 Pages: 489-495

    • DOI

      10.1016/j.jbiosc.2014.05.002

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Complete genome sequence of Bifidobacterium longum 105-A, a strain with high transformation efficiency.2014

    • Author(s)
      Kanesaki, Y., Masutani, H., Sakanaka, M., Shiwa, Y., Fujisawa, T., Nakamura, Y., Yokota, A., Fukiya, S., Suzuki, T., Yoshikawa, H.
    • Journal Title

      Genome Announc.

      Volume: 2 Issue: 6

    • DOI

      10.1128/genomea.01311-14

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 腸内生存に寄与するビフィズス菌遺伝子の探索系の構築2016

    • Author(s)
      中川路伸吾,阪中幹祥,横田篤,吹谷智
    • Organizer
      日本農芸化学会 2016年度大会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] トランスポゾン変異導入系を用いたビフィズス菌の腸内生存に寄与する遺伝子の探索系の構築2016

    • Author(s)
      中川路伸吾,阪中幹祥,横田篤,吹谷智
    • Organizer
      第10回 日本ゲノム微生物学会 年会
    • Place of Presentation
      東京工業大学 大岡山キャンパス(東京都目黒区)
    • Year and Date
      2016-03-04
    • Related Report
      2015 Annual Research Report
  • [Presentation] ビフィズス菌における実用的な遺伝子変異導入系の開発2015

    • Author(s)
      吹谷 智
    • Organizer
      第67回 日本生物工学会大会
    • Place of Presentation
      城山観光ホテル(鹿児島県鹿児島市)
    • Year and Date
      2015-10-26
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] INSeq 法を用いた腸内生存に寄与するビフィズス菌遺伝子の探索系の構築2015

    • Author(s)
      中川路伸吾、阪中幹祥、横田篤、吹谷智
    • Organizer
      日本乳酸菌学会 2015年度大会
    • Place of Presentation
      和洋女子大学(千葉県市川市)
    • Year and Date
      2015-07-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] IS3 family 挿入配列ISBlo11 を用いたビフィズス菌のトランスポゾン変異導入系の確立2015

    • Author(s)
      阪中幹祥,横田 篤,吹谷 智
    • Organizer
      第9回日本ゲノム微生物学会年会
    • Place of Presentation
      神戸大学神大会館(兵庫県神戸市)
    • Year and Date
      2015-03-06 – 2015-03-08
    • Related Report
      2014 Research-status Report
  • [Presentation] 転移因子ISBlo11を利用したビフィズス菌のトランスポゾン変異導入系の開発2014

    • Author(s)
      阪中幹祥,玉井早紀,平山洋佑,横田 篤,吹谷 智
    • Organizer
      平成26年度 公益社団法人 日本農芸化学会 北海道支部講演会
    • Place of Presentation
      北海道大学学術交流会館(北海道札幌市)
    • Year and Date
      2014-12-13
    • Related Report
      2014 Research-status Report
  • [Presentation] Identification of bifidobacterial promoters that are suitable for transposon mutagenesis in bifidobacteria.2014

    • Author(s)
      Mikiyasu Sakanaka, Saki Tamai, Yosuke Hirayama, Atsushi Yokota and Satoru Fukiya
    • Organizer
      The 11th International Symposium on Lactic Acid Bacteria
    • Place of Presentation
      Hotel Zuiderduin (The Netherlands)
    • Year and Date
      2014-08-31 – 2014-09-04
    • Related Report
      2014 Research-status Report
  • [Presentation] 高活性型ビフィズス菌プロモーターの同定とトランスポゾン変異導入系への利用2014

    • Author(s)
      阪中幹祥,玉井早紀,平山洋佑,横田 篤,吹谷 智
    • Organizer
      第8回細菌学若手コロッセウム
    • Place of Presentation
      ホテルニセコいこいの村(北海道ニセコ町)
    • Year and Date
      2014-08-06 – 2014-08-08
    • Related Report
      2014 Research-status Report
  • [Presentation] 大腸菌低活性型のビフィズス菌プロモーターの同定およびトランスポゾン変異導入系への応用2014

    • Author(s)
      阪中幹祥,玉井早紀,平山洋佑,横田 篤,吹谷 智
    • Organizer
      日本乳酸菌学会2014年度大会
    • Place of Presentation
      メルパルク広島(広島県広島市)
    • Year and Date
      2014-07-17 – 2014-07-18
    • Related Report
      2014 Research-status Report
  • [Presentation] 大腸菌およびBifidobacterium longumにおけるビフィズス菌プロモーターの機能解析2014

    • Author(s)
      阪中幹祥,玉井早紀,平山洋佑,横田 篤,吹谷 智
    • Organizer
      第8回日本ゲノム微生物学会年会
    • Place of Presentation
      東京農業大学(東京都世田谷区)
    • Related Report
      2013 Research-status Report
  • [Presentation] ビフィズス菌のランダム変異導入系に適したプロモーターの同定2013

    • Author(s)
      阪中幹祥,平山洋佑,吹谷 智,加納康正,横田 篤
    • Organizer
      2013年度 日本乳酸菌学会泊まり込みセミナー
    • Place of Presentation
      リゾートホテル蓼科(長野県茅野市)
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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