| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Outline of Final Research Achievements |
Intracellular coenzyme A (CoA), which functions as an acyl carrier in vivo, was increased using pantothenate kinase (CoaA) in Escherichia coli, with the ultimate aim of applying this cofactor engineering to the production of a useful compound. The E. coli CoA biosynthetic pathway was most efficiently enhanced by using a prokaryotic type III CoaA, and more than 70% of the formed CoA pool was accumulated as acetyl-CoA. The addition of pantothenate at millimolar concentrations to the culture medium was indispensable for enlarging the cellular CoA pool size. This effect was also observed when the pantothenate precursors, β-alanine and pantoate, were added. Furthermore, the coexpression of the enzymes functioning in the pantothenate biosynthetic pathway together with type III CoaA facilitated the enhancement of the de novo CoA biosynthesis in E. coli cells.
|
