Functional analysis of mature ribosome degradation by RNase T2 in Saccharomyces cerevisiae

• Project/Area Number: 25450093
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: The University of Tokyo
• Principal Investigator: Ogawa Tetsuhiro 東京大学, 農学生命科学研究科, 助教 (40323480)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 出芽酵母 / リボソーム / リボヌクレアーゼ / ストレス応答

Outline of Final Research Achievements

In this study, we tried to elucidate the molecular mechanism of mature ribosome degradation observed in Saccharomyces cerevisiae by the treatment of rapamycin, an antibiotic inducing cellular starvation response. 18S and 25S ribosomal RNA (rRNA) is degraded by Rny1p, which belongs to the conserved RNase T2 family. Ribosomal proteins are also degraded in the same condition. These results indicate that mature ribosomes are actually degraded by the rapamycin treatment, which results in adaptation of host cells to the starvation condition. The degradation partly occurs in the vacuole, which depends on non-selective autophagy pathway. However, most of ribosomes seem to be degraded outside the vacuole.

Research Products

• [Journal Article] Transfer-messenger RNA and SmpB mediate bacteriostasis in Escherichia coli cells against tRNA cleavage. (2015)
  • Author(s): Sakai, F., Sugita,R., Chang, J.-W., Ogawa, T., Hidaka, M., Masaki, H.
  • Journal Title: Microbiology Volume: 161 Issue: 10 Pages: 2019-2028
  • DOI: 10.1099/mic.0.000144
  • Peer Reviewed
• [Journal Article] Mitochondrial tRNA cleavage by tRNA-targeting ribonuclease causes mitochondrial dysfunction observed in mitochondrial disease. (2014)
  • Author(s): Ogawa T, Shimizu A, Takahashi K, Hidaka M, Masaki H.
  • Journal Title: Biochem Biophys Res Commun Volume: 451 Issue: 1 Pages: 131-136
  • DOI: 10.1016/j.bbrc.2014.07.084
  • Peer Reviewed
• [Journal Article] Evidence for DNA cleavage caused by a transfer-tRNA-targeting toxin. (2013)
  • Author(s): Shigematsu, M., Ogawa, T., Tanaka, W., Takahashi, K., Kitamoto, H.K., Hidaka, M, and Masaki, H,
  • Journal Title: PLoS ONE Volume: 8 Issue: 9 Pages: 75512-75512
  • DOI: 10.1371/journal.pone.0075512
  • Peer Reviewed
• [Presentation] ラパマイシンに応答した出芽酵母リボソーム分解機構の解析 (2016)
  • Author(s): 赤川博文、陣内凱、島日佳理、大石早希子、大本哲也、小川哲弘、日高真誠、正木春彦
  • Organizer: 日本農芸化学会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2016-03-27
• [Presentation] 出芽酵母RNase T2が関与するリボソーム分解機構の解析 (2015)
  • Author(s): 陣内 凱、島 日佳理、大石 早希子、大本 哲也、小川 哲弘、日高 真誠、正木 春彦
  • Organizer: 日本農芸化学会
  • Place of Presentation: 岡山大学
  • Year and Date: 2015-03-28
• [Presentation] 出芽酵母RNase T2を介したストレス応答機構の解析 (2014)
  • Author(s): 陣内 凱、島 日佳理、大石 早希子、大本 哲也、小川 哲弘、日高 真誠、正木 春彦
  • Organizer: 日本農芸化学会
  • Place of Presentation: 明治大学生田キャンパス