| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Outline of Final Research Achievements |
In this study, we tried to elucidate the molecular mechanism of mature ribosome degradation observed in Saccharomyces cerevisiae by the treatment of rapamycin, an antibiotic inducing cellular starvation response. 18S and 25S ribosomal RNA (rRNA) is degraded by Rny1p, which belongs to the conserved RNase T2 family. Ribosomal proteins are also degraded in the same condition. These results indicate that mature ribosomes are actually degraded by the rapamycin treatment, which results in adaptation of host cells to the starvation condition. The degradation partly occurs in the vacuole, which depends on non-selective autophagy pathway. However, most of ribosomes seem to be degraded outside the vacuole.
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