| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
We developed a new pull-down method to detect substrates for the processing protease of Japanese representative industry microbe, Aspergillus oryzae. According to the method, we detected a candidate protein. The protein was an orthologue of yeast polyphosphate kinase. The enzyme is reported concerning with responses to the oxidative stress and starvation stress and maintenance of the intracellular phosphate concentration in yeast. It had been thought deletion of A. oryzae kexin gene caused defective processing of the proteins related to the cell wall synthesis, and it gave rise to growth suppression. However, our results seemed to indicate kexin activated the important proteins involved in the homeostatic system in A. oryzae different from previously what we though.
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