| Project/Area Number |
25450097
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Niigata University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
WATANABE Takeshi 新潟大学, 自然科学系, 教授 (10201203)
MIYAMOTO Katushiro 大阪薬科大学, 薬学部, 准教授 (40231625)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 遺伝子発現 / small RNA / キチナーゼ / 応用微生物学 |
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| Outline of Final Research Achievements |
Small RNA ChiX was expressed constitutively and repressed the translational initiation of chiR mRNA, encoding the transcriptional factor for the expression of chitin-degrading enzymes, by base paring in Serratia marcescens. When the chiPQ-ctb operon, encoding chitoporin and chitobiase, was fully expressed by degradation products of chitin, ChiX bound to the chiP mRNA. Base pairing between ChiX and the chiP mRNA relieved repression on the translational initiation of the chiR mRNA. Thus, the expressed ChiR activated the production of chitin-degrading enzymes. ChiX coordinates the synchronized expression of chitin-degrading enzymes and the system for incorporating chitin degradation products.
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