Comparative genomic analysis of intestinal bacteria involved in isoflavone metabolism

• Project/Area Number: 25450098
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Gifu University
• Principal Investigator: Yokoyama Shin-ichiro 岐阜大学, 応用生物科学部, 研究員 (30291008)
• Co-Investigator(Kenkyū-buntansha): 鈴木 徹 岐阜大学, 応用生物科学部, 教授 (20235972)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 腸内細菌 / エクオール / イソフラボン / ゲノム / 遺伝子マーカー / equol代謝遺伝子 / リアルタイムPCR / 糞便検体 / equol / O-DMA / enteric bacteria / genome / daidzein / 遺伝子

Outline of Final Research Achievements

Nucleotide sequences of 3 equol-producing bacteria derived from culture collection were analyzed and comparative genome analysis was performed together with 2 other known bacterial genomes and 2 gene cluster related to equol metabolism. The comparative genome analysis indicated that the genes are divided into 3 phylogenic groups. In addition, PCR primers were designed from the gene cluster and real time PCR was carried out using the bacterial genomes as templates, it was confirmed that amplification was carried out with the same efficiency. Furthermore, feces of 5 equol producers and 5 non-producers were collected and amplification was attempted using the total extracted genome as a template, using the same primers, amplification of only equol producers was confirmed.

Research Products

• [Journal Article] Stereochemical determination of O-desmethylangolensin produced from daidzein (2015)
  • Author(s): Niwa, T., Yokoyama, S., Matsugasaki, N., Inomata, E., Taira, A., Osawa, T.
  • Journal Title: Food Chemistry Volume: 171 Pages: 153-156
  • DOI: 10.1016/j.foodchem.2014.08.122
  • Peer Reviewed
• [Presentation] Eggerthella sp. YY7918のdaidzein reductade の酵素学的性質 (2016)
  • Author(s): 川田結花
  • Organizer: 日本農芸化学会2016年度大会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2016-03-27
• [Presentation] Eggerthella sp. YY7918由来のエクオール産生酵素ダイゼインレダクターゼの酵素学的性質 (2015)
  • Author(s): 五島 智子、川田 結花、横山 慎一郎、鈴木 徹
  • Organizer: 日本農芸化学会2015年年度大会
  • Place of Presentation: 岡山大学
  • Year and Date: 2015-03-27 – 2015-03-29
• [Presentation] Eggerthella sp. YY7918株のジヒドロダイゼインラセマーゼの解析 (2015)
  • Author(s): 川田 結花、五島 智子、横山 慎一郎、丹羽 利夫、鈴木 徹
  • Organizer: 日本農芸化学会2015年年度大会
  • Place of Presentation: 岡山大学
  • Year and Date: 2015-03-27 – 2015-03-29
• [Presentation] O-desmethylangolensin産生菌SY8519株の代謝に関する研究 (2015)
  • Author(s): 丹羽 利夫、横山 慎一郎、鈴木 徹、大澤 俊彦
  • Organizer: 日本農芸化学会2015年年度大会
  • Place of Presentation: 岡山大学
  • Year and Date: 2015-03-27 – 2015-03-29
• [Presentation] イソフラボンの生理活性に影響を及ぼす腸内細菌 (2015)
  • Author(s): 横山慎一郎、鈴木徹、丹羽利夫、服部正平、大澤俊彦、江崎孝行
  • Organizer: 第88回 日本細菌学会総会
  • Place of Presentation: 長良川国際会議場
  • Year and Date: 2015-03-26 – 2015-03-28
  • Invited
• [Presentation] Intestinal bacteria determine phytoestrogen level affecting bone density (2014)
  • Author(s): Shin-ichiro Yokoyama
  • Organizer: Workshop on “Probiotics and human health in severe environments such as space habitat”
  • Place of Presentation: 大阪大学中之島センター
  • Invited
• [Presentation] Eggerthella sp. YY7918株でのダイゼイン-エクオール変換に関する酵素の解析 (2014)
  • Author(s): 川田結花、横山慎一郎、野村泉、服部正平、大島健志朗、鈴木徹
  • Organizer: 日本農芸化学会
  • Place of Presentation: 明治大学生田キャンパス
• [Presentation] 大豆食と腸内細菌 (2013)
  • Author(s): 川田結花、柳瀬笑子、服部正平、大島健志朗、横山慎一郎、野村泉、長野宏子、鈴木徹
  • Organizer: 日本調理科学会
  • Place of Presentation: 奈良女子大学
• [Presentation] Eggerthella sp. YY7918株におけるダイゼイン-エクオール変換酵素系の解析 (2013)
  • Author(s): 川田結花、服部正平、大島健志朗、柳瀬笑子、野村泉、横山慎一郎、鈴木徹
  • Organizer: 日本生物工学会
  • Place of Presentation: 広島国際会議場