| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Nucleotide sequences of 3 equol-producing bacteria derived from culture collection were analyzed and comparative genome analysis was performed together with 2 other known bacterial genomes and 2 gene cluster related to equol metabolism. The comparative genome analysis indicated that the genes are divided into 3 phylogenic groups. In addition, PCR primers were designed from the gene cluster and real time PCR was carried out using the bacterial genomes as templates, it was confirmed that amplification was carried out with the same efficiency. Furthermore, feces of 5 equol producers and 5 non-producers were collected and amplification was attempted using the total extracted genome as a template, using the same primers, amplification of only equol producers was confirmed.
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