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Analysis of organic solvent tolerant mechanism triggered by mutation of transcription factor in yeast

Research Project

Project/Area Number 25450099
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied microbiology
Research InstitutionKyoto University

Principal Investigator

Kuroda Kouichi  京都大学, (連合)農学研究科(研究院), 准教授 (30432339)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords有機溶媒耐性 / 転写因子 / ABCトランスポーター / 細胞壁タンパク質 / パン酵母
Outline of Final Research Achievements

The applicant have found that one point mutation of transcription factor Pdr1p is important for organic-solvent tolerance by analyzing organic-solvent-tolerant yeast strain KK-211 that was isolated through serial culture. In this study, the applicant identified the proteins that cause directly organic-solvent-tolerance among the upregulated cell membrane and cell wall proteins in Pdr1p mutant. In addition, it was found that the identified proteins differed depending on the features of hydrophobic or hydrophilic organic solvent. By analyzing the involvement of Pdr3p, that is a paralog of Pdr1p, in the signal transduction of organic-solvent stress, Pdr3p was demonstrated to play a role in the signal transduction. Furthermore, the applicant proposed the mechanism in which signal of organic-solvent stress was transmitted to Pdr3p via mitochondria.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (12 results)

All 2015 2014 2013 Other

All Journal Article (6 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (6 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Activation of the mitochondrial signaling pathway in response to organic solvent stress in yeast.2015

    • Author(s)
      Nishida-Aoki, N., Mori, H., Kuroda, K., and Ueda, M.
    • Journal Title

      Current genetics

      Volume: 61 Issue: 2 Pages: 153-164

    • DOI

      10.1007/s00294-014-0463-9

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] 高効率物質生産に向けた転写因子デザインによるストレス耐性工学2015

    • Author(s)
      里村淳、黒田浩一、植田充美
    • Journal Title

      日本生物工学会誌

      Volume: 93 Pages: 539-541

    • Related Report
      2015 Annual Research Report
    • Open Access
  • [Journal Article] Environmental stress tolerance engineering by modification of cell surface and transcription factor in Saccharomyces cerevisiae2015

    • Author(s)
      Atsushi Satomura, Kouichi Kuroda, Mitsuyoshi Ueda
    • Journal Title

      Current Environmental Engineering

      Volume: -

    • Related Report
      2014 Research-status Report
  • [Journal Article] Activation of signaling pathways related to cell wall integrity and multidrug resistance by organic solvent in Saccharomyces cerevisiae2014

    • Author(s)
      Nao Nishida, Dongyu Jing, Kouichi Kuroda, Mitsuyoshi Ueda
    • Journal Title

      Current genetics

      Volume: 60 Issue: 3 Pages: 149-162

    • DOI

      10.1007/s00294-013-0419-5

    • Related Report
      2014 Research-status Report 2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] ABC transporters and cell wall proteins involved in organic solvent tolerance in Saccharomyces cergvisiae2013

    • Author(s)
      Nao Nishida, Naoki Ozato, Ken Matsui, Kouichi Kuroda, Mitsuvoshi Ueda.
    • Journal Title

      Journal of biotechnology

      Volume: 165 Issue: 2 Pages: 145-152

    • DOI

      10.1016/j.jbiotec.2013.03.003

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] 包括的転写制御による効率的物質生産に向けたストレス耐性育種2013

    • Author(s)
      黒田浩一, 植田充美
    • Journal Title

      日本生物工学会誌

      Volume: 91 Pages: 342-245

    • NAID

      110009616011

    • Related Report
      2013 Research-status Report
  • [Presentation] Mitochondria-mediated signaling pathway involved in stress response to organic solvent in yeast2015

    • Author(s)
      Kouichi Kuroda, Nao Nishida-Aoki, Hitoshi Mori, Mitsuyoshi Ueda
    • Organizer
      115th American Society for Microbiology (ASM) General Meeting
    • Place of Presentation
      New Orleands, LA, USA
    • Year and Date
      2015-06-01
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Mitochondria-mediated Signaling pathway Involved in stress response to organic solvent in yeast2015

    • Author(s)
      Kouichi Kuroda, Nao Nishida-Aoki, Hitoshi Mori, Mitsuyoshi Ueda
    • Organizer
      115th American Society for Microbiology (ASM) General Meeting
    • Place of Presentation
      New Orleands, LA, USA
    • Year and Date
      2015-06-01
    • Related Report
      2014 Research-status Report
  • [Presentation] Organic solvent-activated signaling pathways via cell surface sensors and transcription factors2014

    • Author(s)
      Kouichi Kuroda, Nao Nishida, Mitsuyoshi Ueda
    • Organizer
      American Society for Biochemistry and Molecular Biology (ASBMB) Annual Meeting
    • Place of Presentation
      San Diago, CA, USA
    • Year and Date
      2014-04-27
    • Related Report
      2014 Research-status Report
  • [Presentation] Effects of recognition sequence variations on transcription regulation of multidrug resistance regulator Pdr1p in yeast

    • Author(s)
      Nao Nishida, Kouichi Kuroda, Mitsuyoshi Ueda
    • Organizer
      American Society for Biochemistry and Molecular Biology (ASBMB) Annual Meeting
    • Place of Presentation
      Boston, MA, USA
    • Related Report
      2013 Research-status Report
  • [Presentation] 酵母の多剤耐性と有機溶媒耐性機構の共通性と違いについて

    • Author(s)
      西田奈央, 黒田浩一, 植田充美
    • Organizer
      第36回 日本分子生物学会年会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2013 Research-status Report
  • [Presentation] Organic solvent-activated signaling pathways via cell surface sensors and transcriptin factors

    • Author(s)
      Kouichi Kuroda, Nao Nishida, Mitsuyoshi Ueda
    • Organizer
      American Society for Biochemistry and Molecular Biology (ASBMB) Annual Meeting
    • Place of Presentation
      San Diego, CA, USA
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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