Multifaceted approaches for elucidation of the ultraviolet receptor for ultraviolet-sensing in phytopathogenic fungi

• Project/Area Number: 25450101
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Shimane University
• Principal Investigator: Kihara Junichi 島根大学, 生物資源科学部, 教授 (40294368)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 紫外線 / 光受容体 / 光形態形成 / メラニン / 分生胞子形成 / 遺伝子発現 / UVB / イネごま葉枯病菌 / 植物病原糸状菌 / 胞子形成

Outline of Final Research Achievements

Bipolaris oryzae is the causal agent of brown leaf spot disease in rice, and its asexual spore formation is known to be induced by near-ultraviolet (NUV) irradiation. We identified 46 genes up-regulated by NUV irradiation in B. oryzae. These genes were categorized as either (1) blue-light receptor (BLR1)-dependent or (2) BLR1-independent expression groups. In addition, we isolated and characterized the T4HR1 gene encoding T4HN reductase in melanin biosynthesis in B. oryzae. A quantitative real-time PCR analysis showed that the expression of T4HR1 transcripts was enhanced by NUV irradiation and regulated by the transcriptional factor BMR1, similar to other melanin biosynthesis genes. On the other hand, analysis using a light emitting diode (LED) that emits ultraviolet radiation suggested that an unknown UVB photoreceptor could participate in the asexual spore formation and in the transcriptional regulation of the genes up-regulated by NUV irradiation in B. oryzae.

Research Products

• [Journal Article] Expression of T4HR1, a 1,3,6,8-tetrahydroxynaphthalene reductase gene involved in melanin biosynthesis, is enhanced by near-ultraviolet irradiation in Bipolaris oryzae (2015)
  • Author(s): Tanaka N, Haruki Y, Ueno M, Arase S, Kihara J
  • Journal Title: Advances in Microbiology Volume: 5 Issue: 03 Pages: 166-176
  • DOI: 10.4236/aim.2015.53016
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Identification and Expression Analysis of Regulatory Genes Induced by Near-Ultraviolet Irradiation in Bipolaris oryzae (2014)
  • Author(s): Kihara J, Tanaka N, Ueno M, Arase S
  • Journal Title: Advances in Microbiology Volume: 4 Issue: 05 Pages: 233-241
  • DOI: 10.4236/aim.2014.45030
  • Peer Reviewed