| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
Bipolaris oryzae is the causal agent of brown leaf spot disease in rice, and its asexual spore formation is known to be induced by near-ultraviolet (NUV) irradiation. We identified 46 genes up-regulated by NUV irradiation in B. oryzae. These genes were categorized as either (1) blue-light receptor (BLR1)-dependent or (2) BLR1-independent expression groups. In addition, we isolated and characterized the T4HR1 gene encoding T4HN reductase in melanin biosynthesis in B. oryzae. A quantitative real-time PCR analysis showed that the expression of T4HR1 transcripts was enhanced by NUV irradiation and regulated by the transcriptional factor BMR1, similar to other melanin biosynthesis genes. On the other hand, analysis using a light emitting diode (LED) that emits ultraviolet radiation suggested that an unknown UVB photoreceptor could participate in the asexual spore formation and in the transcriptional regulation of the genes up-regulated by NUV irradiation in B. oryzae.
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