Application of a phosphite dehydrogenase gene as a dominant selection marker for a large-scale cultivation of microorganisms
| Project/Area Number |
25450102
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied microbiology
|
|---|
| Research Institution | Hiroshima University |
|---|
Principal Investigator |
Ryuichi Hirota 広島大学, 先端物質科学研究科, 助教 (90452614)
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | 亜リン酸 / 選択マーカー / 微生物 / 培養 / 亜リン酸デヒドロゲナーゼ / NAD(P)H |
|---|
| Outline of Final Research Achievements |
The use of antibiotic resistance markers in the commercial application of genetically modified microorganisms is limited due to restrictions on the release of antibiotics and their resistance genes to the environment. To avoid contamination by other microorganisms, the development of a dominant selection marker with low environmental risks is still required. In this study, we developed a novel selection system for microorganisms using a bacterial phosphite dehydrogenasegene (PtxD). Introduction of PtxD gene into a fission yeast Schizosaccharomyces pombe, a budding yeast Saccharomyces cerevisiae, and a Gram-negative bacterium Escherichia coli could confer the ability to grow on a minimal medium containing phosphite (Pt) as a sole source of phosphorus. Since Pt is a safe and inexpensive chemical, ptxD could be used as a novel dominant selection marker applicable to a large-scale cultivation of microorganisms.
|
Report
(4 results)
Research Products
(25 results)
-
-
-
-
[Journal Article] Discovery of phosphonic acid natural products by mining the genomes of 10,000 Actinomycetes2015
Author(s)
K.-S. Ju, J. Gao, J. R. Doroghazi, K.-K. Wang, C. Thibodeaux, S. Li, E. Metzger, J. Fudala, J. Su, J. Zhang, J. P. Cioni, J. Lee, B. S. Evans, R. Hirota, D. P. Labeda, W. A. van der Donk, W. W. Metcalf
-
Journal Title
Proc. Nat. Acad. Sci. USA.
Volume: 112
Issue: 39
Pages: 12175-12180
DOI
Related Report
Peer Reviewed / Int'l Joint Research
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Discovery of Novel Phosphonate Natural Products and Their Biosynthetic Pathways by Large-Scale Genome Mining2014
Author(s)
K.-S. Ju, J. Gao, J. Doroghazi, S. Li, E. Metzger, J. Fudala, J. Su, J. Zhang, J. Lee, J. Cioni, B. Evans, R. Hirota, D. Labeda, W. van der Donk and W. Metcalf
Organizer
65th Annual Meeting of Society for Industrial Microbiology & Biotechnology
Place of Presentation
St. Louis, USA
Year and Date
2014-07-22
Related Report
-
-
-
-
-
-
-
