| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
The use of antibiotic resistance markers in the commercial application of genetically modified microorganisms is limited due to restrictions on the release of antibiotics and their resistance genes to the environment. To avoid contamination by other microorganisms, the development of a dominant selection marker with low environmental risks is still required. In this study, we developed a novel selection system for microorganisms using a bacterial phosphite dehydrogenasegene (PtxD). Introduction of PtxD gene into a fission yeast Schizosaccharomyces pombe, a budding yeast Saccharomyces cerevisiae, and a Gram-negative bacterium Escherichia coli could confer the ability to grow on a minimal medium containing phosphite (Pt) as a sole source of phosphorus. Since Pt is a safe and inexpensive chemical, ptxD could be used as a novel dominant selection marker applicable to a large-scale cultivation of microorganisms.
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