| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
The plant pathogenic bacterium Ralstonia solanacearum injects more than 70 effector proteins into the host plant cells via needle-like structure of a type Ⅲ secretion system. The effector proteins manipulate host cellular functions with diverse molecular activities. The understanding of the molecular function of effectors is essential to uncover the molecular mechanism for pathogenesis of R. solanacearum-infection. Here, we show that a ChaC-like domain containing effector RipAY from R. solanacearum exhibits γ-glutamyl cyclotransferase (GGCT) activity to degrade the major intracellular redox buffer, glutathione, which is known to be crucial for a proper activation of host immune defense in plants. Intriguingly, RipAY protein exhibits a robust GGCT activity by the interaction with eukaryotic thioredoxins, which are also important for intracellular redox homeostasis during bacterial infection in plants.
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