Characterization of transcriptional factors involved in the unique properties of a white koji mold
| Project/Area Number |
25450106
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Kagoshima University |
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Principal Investigator |
FUTAGAMI Taiki 鹿児島大学, 農水産獣医学域農学系, 准教授 (60512027)
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| Co-Investigator(Kenkyū-buntansha) |
GOTO Masatoshi 九州大学, 大学院農学研究院, 准教授 (90274521)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 白麹菌 / Aspergillus / 転写制御因子 / クエン酸 / 分生子 / Aspergillus kawachii |
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| Outline of Final Research Achievements |
The transcriptional change of a white koji mold, Aspergillus luchuensis mut. kawachii, during barley koji production was investigated and 7 putative transcriptional factor encoding genes (nosA、rosA、atfA、atfB、AKAW_01957, AKAW_02766, AKAW_02903) were identified among the genes with significant transcriptional changes. These genes were disrupted and the nosA and rosA double disruptant showed radial colony growth similar to the wild type strain, but showed reduced conidia formation. It was also indicated that the NosA-GFP and RosA-GFP fusion proteins localized in the nuclei.
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Report
(4 results)
Research Products
(10 results)
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[Journal Article] Marker recycling system using the sC gene in the white koji mold, Aspergillus luchuensis mut. kawachii2016
Author(s)
Chihiro Kadooka, Shiori Onitsuka, Mizuki Uzawa, Satoshi Tashiro, Yasuhiro Kajiwara, Hideharu Takashita, Kayu Okutsu, Yumiko Yoshizaki, Kazunori Takamine, Masatoshi Goto, Hisanori Tamaki, Taiki Futagami
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Journal Title
The Journal of General and Applied Microbiology
Volume: 62
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Transcriptomic analysis of temperature responses of Aspergillus kawachii during barley koji production.2015
Author(s)
Futagami, T., Mori, K., Wada, S., Ida, H., Kajiwara, Y., Takashita, H., Tashiro, K., Yamada, O., Omori, T., Kuhara, S., and Goto, M.
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Journal Title
Appl. Environ. Microbiol.
Volume: 81
Issue: 4
Pages: 1353-1363
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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