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Characterization of transcriptional factors involved in the unique properties of a white koji mold

Research Project

Project/Area Number 25450106
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied microbiology
Research InstitutionKagoshima University

Principal Investigator

FUTAGAMI Taiki  鹿児島大学, 農水産獣医学域農学系, 准教授 (60512027)

Co-Investigator(Kenkyū-buntansha) GOTO Masatoshi  九州大学, 大学院農学研究院, 准教授 (90274521)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords白麹菌 / Aspergillus / 転写制御因子 / クエン酸 / 分生子 / Aspergillus kawachii
Outline of Final Research Achievements

The transcriptional change of a white koji mold, Aspergillus luchuensis mut. kawachii, during barley koji production was investigated and 7 putative transcriptional factor encoding genes (nosA、rosA、atfA、atfB、AKAW_01957, AKAW_02766, AKAW_02903) were identified among the genes with significant transcriptional changes. These genes were disrupted and the nosA and rosA double disruptant showed radial colony growth similar to the wild type strain, but showed reduced conidia formation. It was also indicated that the NosA-GFP and RosA-GFP fusion proteins localized in the nuclei.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (10 results)

All 2016 2015 2014 2013

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (7 results) Book (1 results)

  • [Journal Article] Marker recycling system using the sC gene in the white koji mold, Aspergillus luchuensis mut. kawachii2016

    • Author(s)
      Chihiro Kadooka, Shiori Onitsuka, Mizuki Uzawa, Satoshi Tashiro, Yasuhiro Kajiwara, Hideharu Takashita, Kayu Okutsu, Yumiko Yoshizaki, Kazunori Takamine, Masatoshi Goto, Hisanori Tamaki, Taiki Futagami
    • Journal Title

      The Journal of General and Applied Microbiology

      Volume: 62

    • NAID

      130005164750

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Transcriptomic analysis of temperature responses of Aspergillus kawachii during barley koji production.2015

    • Author(s)
      Futagami, T., Mori, K., Wada, S., Ida, H., Kajiwara, Y., Takashita, H., Tashiro, K., Yamada, O., Omori, T., Kuhara, S., and Goto, M.
    • Journal Title

      Appl. Environ. Microbiol.

      Volume: 81 Issue: 4 Pages: 1353-1363

    • DOI

      10.1128/aem.03483-14

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Aspergillus属糸状菌のストレス応答に関する研究2016

    • Author(s)
      二神泰基
    • Organizer
      平成27年度(第313回)日本農芸化学会西日本支部講演会
    • Place of Presentation
      九州大学(福岡県福岡市)
    • Year and Date
      2016-01-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] 白麹菌Aspergillus kawachiiにおけるクエン酸合成関連遺伝子の機能解析2015

    • Author(s)
      門岡千尋、泉津弘佑、奥津果優、吉﨑由美子、髙峯和則、後藤正利、玉置尚徳、二神泰基
    • Organizer
      微生物学の新たな発展,ゲノムから機能・実用に関する九州シンポジウム
    • Place of Presentation
      ANAホリデイ・イン リゾート宮崎(宮崎県宮崎市)
    • Year and Date
      2015-12-06
    • Related Report
      2015 Annual Research Report
  • [Presentation] 白麹菌 Aspergillus kawachii におけるクエン酸合成関連遺伝子の機能解析2015

    • Author(s)
      門岡千尋、泉津弘佑、奥津果優、吉﨑由美子、髙峯和則、後藤正利、玉置尚徳、二神泰基
    • Organizer
      第15回糸状菌分子生物学コンファレンス
    • Place of Presentation
      東京都ルミエール府中(東京都府中市)
    • Year and Date
      2015-11-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] 白麹菌におけるクエン酸生産関連遺伝子の解析2015

    • Author(s)
      門岡千尋、泉津弘佑、奥津果優、吉崎由美子、髙峯和則、後藤正利、玉置尚徳、二神泰基
    • Organizer
      第67回日本生物工学会大会
    • Place of Presentation
      鹿児島県城山観光ホテル(鹿児島県鹿児島市)
    • Year and Date
      2015-10-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] 焼酎麹菌のクエン酸高生産機構に関する研究2015

    • Author(s)
      二神泰基
    • Organizer
      第18回真核微生物交流会
    • Place of Presentation
      独立行政法人酒類総合研究所(広島県東広島市)
    • Year and Date
      2015-06-12
    • Related Report
      2015 Annual Research Report
  • [Presentation] 白麹菌における推定転写制御因子 NosA と RosA の解析2014

    • Author(s)
      二神泰基、田代智史、梶原康博、高下秀春、竹川薫、玉置尚徳、後藤正利
    • Organizer
      第14回糸状菌分生物学コンファレンス
    • Place of Presentation
      東北大学川内北キャンパス(宮城県、仙台市)
    • Year and Date
      2014-11-14 – 2014-11-15
    • Related Report
      2014 Research-status Report
  • [Presentation] 麦麹の製造過程における白麹菌のマイクロアレイ解析2013

    • Author(s)
      二神泰基、森一樹、和田正太郎、梶原康博、高下秀春、大森俊郎、田代康介、久原哲、後藤正利
    • Organizer
      第65回日本生物工学会大会
    • Place of Presentation
      広島国際会議場
    • Related Report
      2013 Research-status Report
  • [Book] Cell wall biosynthesis in filamentous fungi2015

    • Author(s)
      Takuji Oka T, Taiki Futagami T, Masatoshi Goto
    • Total Pages
      18
    • Publisher
      Springer Japan
    • Related Report
      2015 Annual Research Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

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