Analysis of seasonal change and identification of the bacteria and phages responsible for the algal blooming by metagenomics method.

• Project/Area Number: 25450108
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Akita Prefectural University
• Principal Investigator: Fukushima Jun 秋田県立大学, 生物資源科学部, 教授 (00181256)
• Co-Investigator(Kenkyū-buntansha): ASANO Ryoki 秋田県立大学, 生物資源科学部, 研究員 (20646137) ; OKANO Kunihiro 秋田県立大学, 生物資源科学部, 助教 (30455927)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: アオコ / ファージ / メタゲノム / 次世代シーケンサ / 次世代シークエンサ / Microcystis / microbiome / virome / Microcystis aeruginosa / Microcystin

Outline of Final Research Achievements

We have been studied of the microbiome and virome in the Hachiroko-lake every month for these three years without freezing period. The algal blooming in the Hachiroko-lake was emerged by the rapid growth of Microcystis cells more than 90% of total bacteria in the middle of July to September in which the water temperature was above 25℃. After the middle of September, the water temperature fell down and the ratio of Microcystis rapidly decreased bellow 10% of total bacteria. Our result represents the possibility of the participation of Microcystis phage for this reduction.

Research Products

• [Journal Article] Nurmi-type Culture Prepared using Culture Media without _L-Cysteine Enhances <i>Salmonella </i>Exclusion in Hatched Layer Chicks (2016)
  • Author(s): Yoichiro Shimura Nobuo Shoji Taichiro Tanikawa Tomoaki Obayashi Jun Honda Miyuki Tanaka Yukiko Sasaki Jun Fukushima Tamio Inamoto
  • Journal Title: The Journal of Poultry Science Volume: 53 Issue: 2 Pages: 165-172
  • DOI: 10.2141/jpsa.0150101
  • NAID: 130005147707
  • ISSN: 1346-7395, 1349-0486
  • Peer Reviewed / Open Access
• [Journal Article] Complete Genome Sequence of the Unclassified Iron-Oxidizing, Chemolithoautotrophic Burkholderiales Bacterium GJ-E10, Isolated from an Acidic River (2015)
  • Author(s): Fukushima, J. Tojo, F. Asano, R. Kobayashi, Y. Shimura, Y. Okano, K. Miyata, N.
  • Journal Title: Genome Announcements Volume: 3 Issue: 1
  • DOI: 10.1128/genomea.01455-14
  • Peer Reviewed / Open Access
• [Journal Article] The sea water inundation by the Tohoku tsunami caused by the great east-Japan earthquake 2011, leaves strong impact for soil bacterial community after more than one year. (2013)
  • Author(s): Asano, R., Y. Nakai, W. Kawada, Y. Shimura, T. Inamoto, and J. Fukushima
  • Journal Title: Microbial Ecology Volume: 66 Issue: 3 Pages: 639-646
  • DOI: 10.1007/s00248-013-0261-9
  • Peer Reviewed
• [Presentation] 真菌Acremonium strictumのマンガン酸化酵素の遺伝子クローニング (2015)
  • Author(s): 宮田 直幸 北山阿有美 東條ふゆみ 小林 弥生 福島 淳 谷 幸則
  • Organizer: 日本生物工学会
  • Place of Presentation: 鹿児島県
  • Year and Date: 2015-10-26
• [Presentation] 東日本大震災の津波浸水による農地土壌微生物群集への長期的影響 (2015)
  • Author(s): 浅野亮樹 早川敦 志村洋一郎 稲元民夫 福島淳
  • Organizer: 日本微生物生態学会
  • Place of Presentation: 茨城県
  • Year and Date: 2015-10-17
• [Presentation] The mechanism of membrane vesicle production in Pseudomonas aeruginosa and its immunological determinant in mice (2015)
  • Author(s): Junya Ohmomo Masatoshi Miyakoshi Yoichiro Shimura Naomasa Gotoh Jun Fukushima
  • Organizer: 14th International Conference on Pseudomonas
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2015-09-08
  • Int'l Joint Research
• [Presentation] 酸性河川より分離された鉄酸化細菌Sulfobacillus thermosulfidooxidans NE106Gのゲノム構造 (2015)
  • Author(s): 福島淳 東條ふゆみ 浅野亮樹 小林弥生 志村洋一郎 岡野邦宏 宮田直幸
  • Organizer: 2015年度農芸化学会大会
  • Place of Presentation: 岡山市
  • Year and Date: 2015-03-28
• [Presentation] 八郎潟における微生物群集構造の周年解析 (2015)
  • Author(s): 浅野亮樹、岡野邦宏、小林弥生、志村洋一郎、福島淳
  • Organizer: ゲノム微生物学会
  • Place of Presentation: 神戸市
  • Year and Date: 2015-03-06
• [Presentation] 酸性環境より分離した新規鉄酸化細菌のゲノム構造から推定される代謝様式 (2014)
  • Author(s): 福島淳 東條ふゆみ 浅野亮樹 小林弥生 志村洋一郎 岡野邦宏 宮田直幸
  • Organizer: 環境微生物系学会合同大会2014
  • Place of Presentation: 浜松市
  • Year and Date: 2014-10-21
• [Presentation] アオコ発生時期の八郎潟における微生物群集構造の解析 (2014)
  • Author(s): 浅野亮樹, 岡野邦宏, 早川敦, 志村洋一郎, 稲元民夫, 福島淳
  • Organizer: 日本ゲノム微生物学会
  • Place of Presentation: 東京農業大学 世田谷キャンパス