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Screening for ethanol fermenting filamentous fungi and application to bioethanol production

Research Project

Project/Area Number 25450109
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied microbiology
Research InstitutionToyama Prefectural University

Principal Investigator

Komeda Hidenobu  富山県立大学, 工学部, 准教授 (50285160)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsバイオエタノール / 糸状菌 / ペントース / Meyerozyma caribbica / エタノール / Mucor / アラビノース
Outline of Final Research Achievements

For the purpose of developing a method capable of high ethanol production from xylose and arabinose in addition to glucose using a filamentous fungi, mutant library was generated and some candidate mutants were obtained and their ethanol productivity was evaluated. Glycerol 3-phosphate phosphatase which is involved in glycerol production during the ethanol fermentation by the fungal strain were purified and characterized. In addition, microorganisms which can metabolize and ferment arabinose were screened from the nature, and a yeast strain was selected. L-arabitol dehydrogenase involved in the arabinose metabolism was purified to homogeneity from the yeast strain and found to have unique substrate specificity.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (14 results)

All 2016 2015 2014 2013 Other

All Int'l Joint Research (1 results) Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (10 results) (of which Int'l Joint Research: 1 results)

  • [Int'l Joint Research] プリンスオブソンクラ大学(タイ)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Identification and Characterization of D-Xylose Reductase Involved in Pentose Catabolism of the Zygomycetous Fungus Rhizomucor pusillus2015

    • Author(s)
      Komeda H, Yamasaki-Yashiki S, Hoshino K, Asano Y
    • Journal Title

      J Biosci Bioeng.

      Volume: 119 Issue: 1 Pages: 57-64

    • DOI

      10.1016/j.jbiosc.2014.06.012

    • NAID

      110009892234

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Molecular Analysis of NAD+-Dependent Xylitol Dehydrogenase from the Zygomycetous Fungus Rhizomucor pusillus and Reversal of the Coenzyme Preference2014

    • Author(s)
      Yamasaki-Yashiki S, Komeda H, Hoshino K, Asano Y
    • Journal Title

      Biosci Biotechnol Biochem

      Volume: 78 Issue: 11 Pages: 1943-1953

    • DOI

      10.1080/09168451.2014.943646

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Identification and characterization of D-xylulokinase from the D-xylose-fermenting fungus, Mucor circinelloides2014

    • Author(s)
      Komeda, H., Yamasaki-Yashiki, S., Hoshino, K. & Asano, Y.
    • Journal Title

      FEMS Microbiol. Lett.

      Volume: 360 Issue: 1 Pages: 51-61

    • DOI

      10.1111/1574-6968.12589

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] タイ由来のアルコール飲料用スターターの微生物叢の解析とアルコール生産糸状菌の探索2016

    • Author(s)
      三澤涼太、米田英伸、高橋裕里香、西田洋巳、Aran H-Kittikun、浅野泰久
    • Organizer
      日本農芸化学会2016年度大会
    • Place of Presentation
      北海道札幌市
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] Purification and characterization of xylitol dehydrogenase with broad substrate specificity from newly isolated pentose fermenting yeast Meyerozyma caribbica2015

    • Author(s)
      Wiphat Sukpipat, Poonsuk Prasertsan, Hidenobu Komeda, Yasuhisa Asano
    • Organizer
      The 2nd Satellite Seminar, International Symposium on Microbial Research and Biotechnology for Biomass Utilization
    • Place of Presentation
      福岡県福岡市
    • Year and Date
      2015-11-12
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Purification and characterization of xylitol dehydrogenase with broad substrate specificity from newly isolated pentose fermenting yeast Meyerozyma caribbica2015

    • Author(s)
      Wiphat Sukpipat, Poonsuk Prasertsan, Hidenobu Komeda, Yasuhisa Asano
    • Organizer
      2015年度日本農芸化学会中部・関西支部合同大会
    • Place of Presentation
      富山県射水市
    • Year and Date
      2015-09-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] タイ由来アルコール飲料用スターター中の微生物叢解析2015

    • Author(s)
      村越美穂、三澤涼太、米田英伸、Aran H-Kittikun、浅野泰久
    • Organizer
      日本農芸化学会2015年度大会
    • Place of Presentation
      岡山
    • Year and Date
      2015-03-27
    • Related Report
      2014 Research-status Report
  • [Presentation] Identification and characterization of D-xylose reductase involved in pentose catabolism of the zygomycetous fungus Rhizomucor pusillus2014

    • Author(s)
      Hidenobu Komeda, Shino Yamasaki-Yashiki, Yasuhisa Asano
    • Organizer
      Active Enzyme Molecule 2014
    • Place of Presentation
      Toyama
    • Year and Date
      2014-12-17 – 2014-12-19
    • Related Report
      2014 Research-status Report
  • [Presentation] タイ及びラオス由来のアルコール発酵用スターターに含まれる微生物叢の解析2014

    • Author(s)
      村越美穂、米田英伸、Aran H-Kittikun、浅野泰久
    • Organizer
      2014年度日本乳酸菌学会泊まり込みセミナー
    • Place of Presentation
      栃木
    • Year and Date
      2014-05-09
    • Related Report
      2014 Research-status Report
  • [Presentation] タイ及びラオス由来スターター中に含まれる微生物叢の解析2014

    • Author(s)
      村越美穂、米田英伸、Aran H-Kittikun、浅野泰久
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      川崎
    • Related Report
      2013 Research-status Report
  • [Presentation] Enzymes involved in pentose metabolism in zygomycetous fungus Mucor circinelloides2013

    • Author(s)
      Hidenobu Komeda, Tatsuya Hamada, Kazuhiro Hoshino, Yasuhisa Asano
    • Organizer
      Enzyme Engineering XXII
    • Place of Presentation
      Toyama
    • Related Report
      2013 Research-status Report
  • [Presentation] Quantitative determination of threonine in human plasma using L-threonine 3-dehydrogenase from Cupriavidus necator2013

    • Author(s)
      Yousuke Tabei, Hidenobu Komeda, Takafumi Sekiguchi, Masaki Takiwaki, Yoshinori Uji, Isao Kitajima, Yasuhisa Asano
    • Organizer
      Enzyme Engineering XXII
    • Place of Presentation
      Toyama
    • Related Report
      2013 Research-status Report
  • [Presentation] Microbial diversity of traditional starters for alcoholic fermentation in Thailand and Laos2013

    • Author(s)
      Miho Murakoshi, Hidenobu Komeda, Aran H-Kittikun, Yasuhisa Asano
    • Organizer
      The 1st International Symposium on Microbial Technology for Food and Energy Security
    • Place of Presentation
      Bangkok
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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