Generation of the cell factories for bio-based organic acid production through improvement of filamentous fungi by modification of transport system
| Project/Area Number |
25450114
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Waseda University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | 応用微生物 / セルファクトリ / クエン酸輸送体 / 酵素 / 代謝工学 / 有機酸 / クエン酸 / trans-アコニット酸 / aconitate isomerase / Aspergillus niger / cell factory / citrate transporter / fermentation / malate citrate shuttle / metabolic engineering / citric acid production / citrate transport |
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| Outline of Final Research Achievements |
For generation of the cell factories for bio-based organic acid production, functional analyses of genes encoding putative mitochondrial citrate transport proteins in citric acid-producing Aspergillus niger were performed. Screening of microorganisms capable of assimilating citric acid as a sole carbon source was also performed, and a novel aconitate isomerase was obtained. trans-Aconitic acid was produced successfully from citric acid by utilization of recombinant E. coli cells heterologously expressing the novel aconitate isomerase gene as biocatalysts for the whole-cell reaction. There is some possibility that heterologous expression of genes encoding novel and specific enzymes related to citrate metabolism in citric acid-producing A. niger as a host and improvement of citrate transport system by genetic engineering will open the way for generation of the cell factories for bio-based organic acid production.
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Report
(4 results)
Research Products
(34 results)
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[Presentation] 高濃度レブリン酸分解菌の単離と解析2015
Author(s)
羽部浩, 佐藤俊, 森田友岳, 福岡徳馬, 小林慶一, 桐村光太郎, 北本大
Organizer
日本農芸化学会2015年度大会
Place of Presentation
岡山
Year and Date
2015-03-26 – 2015-03-29
Related Report
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