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Functional analysis of the transcriptional repressor HapX involved in iron homeostasis in filamentous fungi

Research Project

Project/Area Number 25450116
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied microbiology
Research InstitutionMeijo University

Principal Investigator

Kato Masashi  名城大学, 農学部, 教授 (70242849)

Co-Investigator(Kenkyū-buntansha) SHIMIZU MOTOYUKI  名城大学, 農学部, 助教 (20423535)
Co-Investigator(Renkei-kenkyūsha) KOBAYASHI TETSUO  名古屋大学, 生命農学研究科, 教授 (20170334)
TAKAYA NAOKI  筑波大学, 生命環境科学研究科, 教授 (50282322)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
KeywordsHapX / 鉄の恒常性維持 / ホメオスタシス / CCAAT配列 / Hap複合体 / 転写抑制 / 転写因子 / 転写調節 / 鉄のホメオスタシス / 糸状菌 / CCAAT / 鉄硫黄クラスター / 麹菌 / 鉄の恒常性
Outline of Final Research Achievements

(1) Results of EMSA(Electrophoresis Mobility Shift Assay) with some HapX deletion mutants strongly suggested that the 17 amino acid residues in the N-terminal domain plays an important role in the interaction of HapX and HapB/C/E complex. (2) The results of the in vivo analysis showed that the 17 amino acid residues is required for the function of HapX. (3) We have succeeded in obtaining sufficient amount of the recombinant C-terminal domain containing the Cysteine-rich regions. The results of spectroscopic analysis suggested the existence of iron-sulfur clusters. (4) We established the expression system for a HapX protein with TAP (Tandem Affinity Purification) tag in Aspergillus nidulans. When the HapX was purified under the mild conditions, some proteins interacting with HapX were detected.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (12 results)

All 2015 2014 Other

All Int'l Joint Research (2 results) Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (4 results) (of which Invited: 1 results) Book (2 results) Remarks (1 results)

  • [Int'l Joint Research] Friedrich Schiller University(ドイツ)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] Innsbruck Medical University(オーストリア)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Deciphering the combinatorial DNA-binding code of the CCAAT-binding complex and the iron-regulatory basic region leucine zipper (bZIP) transcription factor HapX.2015

    • Author(s)
      Hortschansky P, Ando E, Tuppatsch K, Arikawa H, Kobayashi T, Kato M, Haas H, Brakhage AA.
    • Journal Title

      J Biol Chem.

      Volume: 290 Issue: 10 Pages: 6058-6070

    • DOI

      10.1074/jbc.m114.628677

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] The Janus transcription factor HapX controls fungal adaptation to both iron starvation and iron excess.2014

    • Author(s)
      Gsaller F, Hortschansky P, Beattie SR, Klammer V, Tuppatsch K, Lechner BE, Rietzschel N, Werner ER, Vogan AA, Chung D, Mühlenhoff U, Kato M, Cramer RA, Brakhage AA, Haas H.
    • Journal Title

      EMBO J.

      Volume: 33 Issue: 19 Pages: 2261-2276

    • DOI

      10.15252/embj.201489468

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 麹菌転写因子HapXによるシデロフォア生産調節機構2014

    • Author(s)
      中村隼人,林口拓実,増田 裕一郎, 安田(吉野)庄子*,北本則行*,志水元亨、加藤雅士
    • Journal Title

      名城大学総合研究所紀要(ISSN 1344-8099)

      Volume: 19

    • Related Report
      2013 Research-status Report
  • [Presentation] 糸状菌における鉄恒常性のマスターレギュレータ HapX の機能解析2015

    • Author(s)
      村田 俊輔、中村 隼人、金子 優平、志水 元亨、加藤 雅士
    • Organizer
      日本農芸化学会2015年度大会
    • Place of Presentation
      岡山大学
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Research-status Report
  • [Presentation] 鉄恒常性に関わる転写因子 HapX と CCAAT-box 結合因子 HapB/C/E との相互作用。2014

    • Author(s)
      中村隼人,金子優平,志水元亨,加藤雅士
    • Organizer
      第14回糸状菌分子生物学コンファレンス
    • Place of Presentation
      東北大学川内北キャンパス
    • Year and Date
      2014-11-15 – 2014-11-16
    • Related Report
      2014 Research-status Report
  • [Presentation] 糸状菌の鉄恒常性に関わる転写因子HapXの機能解析2014

    • Author(s)
      金子 優平、中村 隼人、祖父江 衣純、志水 元享、加藤 雅士
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      明治大学生田キャンパス
    • Related Report
      2013 Research-status Report
  • [Presentation] Fermentation is a modern alchemy: microbes add value to a product.2014

    • Author(s)
      Masashi Kato
    • Organizer
      The 2014 international annual conferences of 4th Basic science in conjunction with 5th global resource conservation.
    • Place of Presentation
      Batu, Indonesia
    • Related Report
      2013 Research-status Report
    • Invited
  • [Book] 発酵・醸造食品の最前線(監修:北本勝ひこ)2015

    • Author(s)
      志水元亨、小林哲夫、加藤雅士
    • Total Pages
      7
    • Publisher
      CMC出版
    • Related Report
      2015 Annual Research Report
  • [Book] 「発酵・醸造食品の最前線(監修:北本勝ひこ)」第18章 ゲノム情報を活用した麹菌の新たな分子育種の可能性。2015

    • Author(s)
      志水元亨、小林哲夫、加藤雅士
    • Total Pages
      282
    • Publisher
      CMC出版
    • Related Report
      2014 Research-status Report
  • [Remarks] 名城大学農学部応用生物化学科 応用微生物学研究室

    • URL

      http://www-agr.meijo-u.ac.jp/labs/nn008/

    • Related Report
      2015 Annual Research Report

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Published: 2014-07-25   Modified: 2019-07-29  

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