| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Since the lipoprotein N-acyltransferase homologs are not found in the genomes of low G+C Gram-positive bacteria, these bacteria are thought to have only N-terminal-free diacylated lipoproteins. However, we have discovered novel types of N-acylated lipoproteins in these bacteria, indicating that they have unusual N-acyltransferases and related enzymes. In this study, mass spectrometry-based method was improved significantly to detect the N-acylation of lipoproteins in a few picomole amount in a day or two. Then, a strain that have only diacylated lipoproteins was sought from the gene disrupted strains and genome deletion mutants of Bacillus subtilis. The search is still continued.
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