| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
We have established a method for isolating intracellular or extracellular symbiotic bacteria that was difficult until now. As a result, we isolated and cultivated intracellular and extracellular symbiotic microorganisms of tubeworm (deep sea organism). Weak electric current was used to efficiently isolate intracellular symbiotic bacteria alive. Confirmation that the isolated bacteria are symbiotic intracellular symbiotic cells of tubeworm was performed with the 16S rRNA gene. Furthermore, many of the strains isolated as extracellular symbiotic bacteria were novel species. Of these, we identified new species of genus Ruegeria, Sulfitobacter and Planktotalea. The adsorptive isolation method based on the weak current used in this study was considered to be one means for culturing difficult-to-cultivate microorganisms in the future.
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