Regulation and role of the arachidonate cascade reaction at different life stages of adipocytes

• Project/Area Number: 25450128
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Shimane University
• Principal Investigator: Yokota Kazushige 島根大学, 生物資源科学部, 教授 (90158361)
• Co-Investigator(Renkei-kenkyūsha): JISAKA Mitsuo 島根大学, 生物資源科学部, 准教授 (60243424) ; NISHIMURA Kohji 島根大学, 生物資源科学部, 助教 (30304257)
• Research Collaborator: SHONO Fumiaki
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: prostaglandin / prostacyclin / adipocyte / adipogenesis / IP receptor / life stage / PGI2 / preadipogenic cells / 前駆脂肪細胞 / 成熟脂肪細胞 / 分化誘導 / プロスタサイクリン / プロスタグランジンI2 / IP受容体 / PPARgamma / 脂肪細胞形成 / 脂肪細胞 / プロスタグランジン / アラキドン酸カスケード / プロスタノイド / シクロオキシゲナーゼ / 6-ケト-PGF1α / 成熟期 / COX-1 / COX-2 / アンチセンス / PGJ2誘導体 / 形質転換細胞

Outline of Final Research Achievements

This study was undertaken to study the regulatory mechanisms of biosynthesis of prostaglandin (PG) I2 called prostacyclin and specific action of its agonists and antagonists in cultured adipocytes at different life stages of adipogenesis including growth, differentiation, and maturation phases. These results revealed the stimulatory effects of endogenous and exogenous PGI2 and the agonists for the IP receptor on cultured preadipogenic cells as well as the suppression by the IP antagonists at differentiation and maturation phases. The findings provided the evidence for the specific and unique roles of prostacyclin in the control of adipogenesis in cultured adipocytes.

Research Products

• [Int'l Joint Research] Noakhali Sci. Tech. Univ./Dhaka University(バングラデシュ)
• [Int'l Joint Research] Noakhali S. and T. University/Dhaka University(バングラデシュ)
• [Journal Article] Stimulation of fat storage by prostacyclin and selective agonists of prostanoid IP receptor during the maturation phase of cultured adipocytes (2016)
  • Author(s): Khan, F., Syeda, P. K., Nartey, M. N. N., Rahman, M. S., Islam, M. S., Nishimura, K., Jisaka, M., Shono, F., and Yokota, K.
  • Journal Title: Cytotechnology Volume: - Issue: 6 Pages: 2417-2429
  • DOI: 10.1007/s10616-016-9960-7
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Pretreatment of cultured preadipocytes with arachidonic acid during the differentiation phase without a cAMP-elevating agent enhances fat storage after the maturation phase (2016)
  • Author(s): Khan, F., Seyda, P. K., Nartey, M. N. N., Rahman, M. S., Islam, M. S., Nishimura, K., Jisaka, M., Shono, F., and Yokota, K.
  • Journal Title: Prostaglandins Other Lipid Mediat. Volume: 123 Pages: 16-27
  • DOI: 10.1016/j.prostaglandins.2016.02.003
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Identification of new flavonol O-glycosides from indigo (Polygonum tinctorium Lour) leaves and their inhibitory activity against 3-hydroxy-3-methylglutaryl CoA reductase (2015)
  • Author(s): Kimura, H., Tokuyama, S., Ishihara, T., Ogawa, S., and Yokota, K.
  • Journal Title: J. Pharm. Biomed. Anal. Volume: 108 Pages: 102-112
  • DOI: 10.1016/j.jpba.2015.02.005
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] タデアイ（藍）葉の新規フラボノイドの同定とコレステロール生合成阻害作用 (2015)
  • Author(s): 横田一成、木村英人、徳山翔太
  • Journal Title: 特産種苗 Volume: 21 Pages: 103-107
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Effect of serum trace elements and macro minerals on myocardial infarction (2015)
  • Author(s): Islam, M. S., Rahaman, M. Z., Uddin, S. M. N., Tammi, T. J., Sarwar, S., and Yokota, K.
  • Journal Title: J. Card. Fail. Volume: 21 Issue: 10 Pages: S195-S195
  • DOI: 10.1016/j.cardfail.2015.08.282
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Endogenous synthesis of prostacyclin was positively regulated during the maturation phase of cultured adipocytes (2014)
  • Author(s): Rahman, M. S., Khan, F., Syeda, P. K., Nishimura, K., Jisaka, M., Nagaya, T., Shono, F., and Yokota, K.
  • Journal Title: Cytotechnology Volume: 66 Issue: 4 Pages: 635-646
  • DOI: 10.1007/s10616-013-9616-9
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Cultured preadipocytes undergoing stable transfection with cyclooxygenase-1 in the antisense direction accelerate adipogenesis during the maturation phase of adipocytes (2013)
  • Author(s): Rahman M. S., Syeda, P. K., Khan F., Yokota, K, et al.
  • Journal Title: Appl. Biochem. Biotechnol. Volume: 171 Issue: 1 Pages: 128-144
  • DOI: 10.1007/s12010-013-0347-3
  • Peer Reviewed
• [Presentation] Exogenous arachidonic acid up-regulates adipogenesis program of cultured preadipocytes during the differentiation phase without a cAMP-elevating agent (2017)
  • Author(s): Nartey, M. N. N., Syeda, P. K., Rahman, M. S., Chowdhury, M. M. I., Nishimura, K., Shimizu, H., Jisaka, M., Shono, F., and Yokota, K
  • Organizer: 47th Annual Meeting of Chu-Shikoku Division in the Japan Society for Bioscience, Biotechnology, and Agrochemistry
  • Place of Presentation: 島根大学松江キャンパス（松江市）
  • Year and Date: 2017-01-28
• [Presentation] Comparison of pro-adipogenic effects between prostaglandin (PG) D2 and its stable isometric analogue, 11-deoxy-11-methylene-PGD2 (2017)
  • Author(s): Syeda, P. K., Rahman, M. S., Chowdhury, M. M. I., Nishimura, K., Shimizu, H., Shono, F., and Yokota, K
  • Organizer: 47th Annual Meeting of Chu-Shikoku Division in the Japan Society for Bioscience, Biotechnology, and Agrochemistry
  • Place of Presentation: 島根大学松江キャンパス（松江市）
  • Year and Date: 2017-01-28
• [Presentation] 1.Stimulation of adipogenesis after the maturation phase by pretreatment of cultured preadipocytes with arachidonic acid during the differentiation phase without a cAMP-elevating agent (2016)
  • Author(s): Khan K, Nartey MNN, Syeda, PK, Rahman MS, Nishimura K, Jisaka M, Shimizu H, Shono F, Yokota K
  • Organizer: The 89th Annual Meeting of the Japanese Biochemical Society
  • Place of Presentation: 仙台国際センター（仙台市）
  • Year and Date: 2016-09-26
• [Presentation] Effect of serum trace elements and macrominerals on myocardial infarction (2015)
  • Author(s): Islam, M. S., Rahaman, M. Z., Uddin, S. M. N., Tammi, T. J., Sarwar, S., and Yokota, K.
  • Organizer: The 19th Japanese Heart Failure Society
  • Place of Presentation: Osaka (Convention Center, Grand Front Osaka)
  • Year and Date: 2015-10-24
• [Presentation] Effect of P2RY12 and CYP2C9 gene polymorphisms on the anti-platelet response of clopidogrel in the Bangladeshi percutaneous coronary intervention patients (2015)
  • Author(s): Islam, M. S., Rahman, M. S., Nartey, M. N. N., and Yokota, K.
  • Organizer: 43th Annual Meeting of Chu-Shikoku Division in the Japan Society for Bioscience, Biotechnology, and Agrochemistry
  • Place of Presentation: Matsuyama (Faculty of Agriculture, Ehime University)
  • Year and Date: 2015-09-18
• [Presentation] 山陰地域の生物資源に由来するポリフェノール類による脂質代謝制御 (2015)
  • Author(s): 横田一成
  • Organizer: 平成27年度油化学会シンポジウムin出雲～健康・美容に貢献する油化学～
  • Place of Presentation: 出雲 （島根大学医学部）
  • Year and Date: 2015-07-11
  • Invited
• [Presentation] タデアイ由来新規フラボノイドの構造決定と3-ヒドロキシ-3-メチルグルタリルCoAレダクターゼ阻害活性 (2015)
  • Author(s): 徳山翔太、木村英人、石原朋恵、小川智史、横田一成
  • Organizer: 日本農芸化学会中四国支部第42回講演会
  • Place of Presentation: 鳥取 （鳥取大学農学部）
  • Year and Date: 2015-06-13
• [Presentation] Effect of PON1, ABCB1C1236T and ABCB1C3435T polymorphisms on the anti-platelet response of clopidogrel in the Bangladeshi percutaneous coronary intervention patients (2015)
  • Author(s): Islam, M. S., Islam, M. S., Apu, M. N. H., Nartey, M. N., Hasnat, A., Yokota, K.
  • Organizer: 42th Annual Meeting of Chu-Shikoku Division in the Japan Society for Bioscience, Biotechnology, and Agrochemistry
  • Place of Presentation: Totter (Faculty of Agriculture, Tottori University)
  • Year and Date: 2015-06-13
• [Presentation] Effect of VKORC1 and CYP2C9 genetic variations on warfarin dose requirement in Bangladeshi population (2015)
  • Author(s): Islam, M. S., Chowdhury, Z. S., Shahjin, F., Khan, F., Fatema, P. K. S. K., Hasnat, A., and Yokota, K.
  • Organizer: 56th Annual Meeting of Chugoku-Shikoku Division in the Japanese Biochemical Society
  • Place of Presentation: Matsue (Faculty of Life and Environmental Science), Shimane University)
  • Year and Date: 2015-05-30
• [Presentation] Up-regulation of prostacyclin biosynthesis and its response after the maturation phase of adipocytes (2014)
  • Author(s): Rahman, M. S., Khan, F., Fatema, P. K. S. K., Islam, M. S., Nishimura, K., Jisaka, M., Nagaya, T., Shono, F., and Yokota, K.
  • Organizer: The 87th Annual Meeting of the Japanese Biochemical Society
  • Place of Presentation: 京都国際会議場（京都市）
  • Year and Date: 2014-10-15 – 2014-10-18
• [Presentation] 11-Deoxy-11-methylene-prostaglandin (PG) D2 exerts pro-adipogenic effect on cultured adipocytes during the maturation phase through cellular mechanism different from that of natural PGD2 (2014)
  • Author(s): Fatema, P. K. S. K., Islam, M. S., Khan, F., Nishimura, K., Jisaka, Nagaya, T., Shono, F., and Yokota, K.
  • Organizer: 40th Annual Meeting of Chu-Shikoku Division in the Japan Society for Bioscience, Biotechnology, and Agrochemistry
  • Place of Presentation: 徳島大学総合科学部（徳島市）
  • Year and Date: 2014-09-27
• [Presentation] Association of GSTP1 and ABCC4 polymorphisms with response and toxicity of cyclophosphamide-based chemotherapy on Bangladeshi breast cancer patients (2014)
  • Author(s): Islam, M. S., Islam, M. S., Khan, F., Fatema, P. K. S., Hasnat, A., and Yokota, K.
  • Organizer: 40th Annual Meeting of Chu-Shikoku Division in the Japan Society for Bioscience, Biotechnology, and Agrochemistry
  • Place of Presentation: 徳島大学総合科学部（徳島市）
  • Year and Date: 2014-09-27
• [Presentation] Biosynthesis of prostacyclin and its action is up-regulated during the maturation phase of adipocytes (2014)
  • Author(s): Khan, F., Rahman, M. S., Fatema, P. K. S. K., Islam, M. S., Nishimura, K., Jisaka, M., Nagaya, T., Shono, F., and Yokota, K.
  • Organizer: 40th Annual Meeting of Chu-Shikoku Division in the Japan Society for Bioscience, Biotechnology, and Agrochemistry
  • Place of Presentation: 徳島大学総合科学部（徳島市）
  • Year and Date: 2014-09-27
• [Presentation] Stable transfection of cultured preadipocytes with cyclooxygenase-1 in the antisense direction up-regulates adipogenesis during the maturation phase of adipocytes (2013)
  • Author(s): Rahman, M. S., Syeda, P. K. Khan, F., Nishimura, K., Jisaka, M., Nagaya, T., Shono, F., and Yokota, K.
  • Organizer: 13th International Conference on Bioactive Lipids in Cancer, Inflammation and Related Diseases
  • Place of Presentation: San Juan, Puerto Rico, USA
• [Presentation] Biosynthesis of prostacyclin serving as a biomarker for enhanced adipogenesis program in cultured adipocytes (2013)
  • Author(s): Rahman, M. S., Khan, F., Syeda, P. K., Nishimura, K., Jisaka, M., Nagaya, T., Shono, F., and Yokota, K.
  • Organizer: 13th International Conference on Bioactive Lipids in Cancer, Inflammation and Related Diseases
  • Place of Presentation: San Juan, Puerto Rico, USA
• [Presentation] Stimulation of adipogenesis with 11-deoxy-11-methylene-PGD2, a stable, isosteric analogue of PGD2, during the maturation phase of adipocytes (2013)
  • Author(s): Fatema, P. K. S. K., Khan, F., Nishimura, K., Jisaka, M., Nagaya, T., Shono, F., and Yokota, K.
  • Organizer: The 86th Annual Meeting of the Japanese Biochemical Society
  • Place of Presentation: Yokohama
• [Presentation] Prostaglandin J2, natural bioactive lipid, modulates adipocyte inflammation. International Conference on Updates on Natural Products in Medicine and Healthcare Systems
  • Author(s): Hossain, M. S., Chowdhury, A. A., Jisaka, M. Nagaya, T., and Yokota, K.
  • Organizer: International Conference on Updates on Natural Products in Medicine and Healthcare Systems
  • Place of Presentation: Khulna, Bangladesh
• [Presentation] Biosynthesis of prostaglandin I2 at different life stages of cultured adipocytes as determined by the immunological assay for its stable hydrolysis product.
  • Author(s): Khan, F., Rahaman, M. S., Syeda, P. K., Nishimura, K., Jisaka, M., Nagaya, T., Shono, F., and Yokota, K.
  • Organizer: 日本農芸化学会中四国支部第36回講演会
  • Place of Presentation: 松江
• [Book] Analysis of highly polymeric proanthocyanidins from seed shells of Japanese horse chestnut and their health benefits. “ Procyanidins: Characterisation, Antioxidant Properties and Health Benefits, Series: Biochemistry Research Trends”, (Edited by Chedea, VS) (2016)
  • Author(s): Yokota K, Kimura H, Ogawa S, Jisaka, M.
  • Total Pages: 11
  • Publisher: Nova Science Publishers, New York,
• [Book] 第１章 技術編 10. 脂質分子に対する免疫原の作製と抗体の産生、遺伝子医学MOOK24号「最新生理活性脂質研究-実験手法、基礎的知識とその応用」 (2013)
  • Author(s): 横田一成
  • Total Pages: 312
  • Publisher: 株式会社メディカルドウ、大阪
• [Remarks] 島根大学教員情報システム
  • URL: https://www.staffsearch.shimane-u.ac.jp/kenkyu/search/detail/975bc27228691c78b9827b5c1a6aa129/research.html
• [Remarks] 大学の研究者情報Webページ
  • URL: https://www.staffsearch.shimane-u.ac.jp/kenkyu/search/detail/975bc27228691c78b9827b5c1a6aa129/research.html