Substrate recognition of bacterial copper-containing amine oxidase possessing broad substrate specifictiy
| Project/Area Number |
25450129
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied biochemistry
|
|---|
| Research Institution | Shimane University |
|---|
Principal Investigator |
SAWA Yoshihiro 島根大学, 生物資源科学部, 教授 (70127489)
|
|---|
| Project Period (FY) |
2013-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
|
|---|
| Keywords | アミンオキシダーゼ / バイオセンサー / 基質認識 / 分子設計 / ヒスタミンオキシダーゼ / in silico解析 / 飽和変異 / DyPペルオキシダーゼ / LRET / モノアミンオキシダーゼ / DyP型ペルオキシダーゼ / In silico 分子設計 / L-アスパラギン酸オキシダーゼ / in silico 分子設計 / in silico |
|---|
| Outline of Final Research Achievements |
We tried to develop biosensor enzymes useful for the specific quantification of physiologically active amines such as GABA, tyramine, histamine, by elucidating the substrate recognition mechanism of two bacterial copper-containing amine oxidases possessing broad substrate specificity.
Unfortunately we could not develop a promising enzyme as a biosensor, but in silico analysis shows a high correlation with biochemical results and it is at a stage where it can be fully utilized for the molecular design of amine oxidase.
|
Report
(5 results)
Research Products
(22 results)
