| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
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| Outline of Final Research Achievements |
We tried to develop biosensor enzymes useful for the specific quantification of physiologically active amines such as GABA, tyramine, histamine, by elucidating the substrate recognition mechanism of two bacterial copper-containing amine oxidases possessing broad substrate specificity.
Unfortunately we could not develop a promising enzyme as a biosensor, but in silico analysis shows a high correlation with biochemical results and it is at a stage where it can be fully utilized for the molecular design of amine oxidase.
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