| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
1) The activity of PCTK3 was increased via interaction with cyclin A and phosphorylation at Ser12 by PKA. 2) PCTK3 knockdown HEK293T cells exhibited an increase in cell size and F-actin accumulation at the edges of cell membranes and increased cofilin phosphorylation at Ser3, suggesting that PCTK3 is involved in the regulation of actin reorganization. 3) PCTK3-knockdown HEK293T cells exhibited an increase in cell motility. 4) The activity of focal adhesion kinase 1 (FAK1), which is activated during early cell adhesion, was suppressed by PCTK3, suggesting that PCTK3 might act as a negative regulator of FAK1.
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