| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
Nuclear-encoded chloroplastic proteins are imported into chloroplasts by utilizing the unique translocation machinery (translocon) embedded in the double-envelope membranes from the cytosol after translated as precursors.
Most of knowledge regarding protein import have been gained through the analyses of the early-translocation intermediates formed under limited energy conditions, in which precursors are trapped in the translocon. However, many questions regarding molecular actions taking place in the early stage of protein import have not been fully understood. In addition, so far as the latter stages of protein translocation are concerned, almost nothing have been solved yet.
During the research period, I have designed and prepared the tools to analyze the protein-protein interactions between precursors and the translocon component(s) and intra-interactions within the translocon at not only the early stage, but also the latter stages of protein import into chloroplasts.
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