| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Hemolytic lectin CEL-III derived from sea cucumber is a pore forming toxin which have hemolytic activity by forming oligomer. To elucidate the mechanism of oligomerization, mutants of CEL-III were made by site directed mutagenesis. The effect of the mutation on the hemolytic activity was elucidated. Production of AML36 and AML49 derived from coral was carried out by using Escherichia coli. However recombinant proteins formed aggregates and difficult to obtain monomeric form which exhibit hemolytic activity. This indicates that the difference of the amino acid sequence between CEL-III and AML36, AML49 affect the hemolytic activity.
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