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New inhibitor for proline specific peptidases which cause several

Research Project

Project/Area Number 25450138
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied biochemistry
Research InstitutionSetsunan University

Principal Investigator

Yoshimoto Tadashi  摂南大学, 理工学部, 教授 (60088870)

Co-Investigator(Kenkyū-buntansha) YONEYAMA Masanori  摂南大学, 薬学部, 講師 (00411710)
Co-Investigator(Renkei-kenkyūsha) NAKAJIMA Yoshitaka  摂南大学, 理工学部, 教授 (80372770)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords歯周病 / 阻害剤 / プロリン / 米ぬか / プロリルトリペプチジルペプチダーゼ / ペプチダーゼ / 糖尿病
Outline of Final Research Achievements

Prolyl tripeptidyl peptidase (PTP) from P. gingivalis is a key enzyme of periodontitis. After screening of natural products, rice bran had high inhibitory activity for PTP. The inhibitor protein (PTPI) was purified from rice bran. By MALDI-TOF MS and database of rice, gene Os05g0323900 was identified as the PTP inhibitor. The protein was expressed as fuson protein with thioredoxin, rPTPI-Trx, in E. coli.,and crystallized by the sitting drop vapor diffusion method

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (8 results)

All 2015 2014 2013

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Acknowledgement Compliant: 1 results) Presentation (4 results) Book (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Prolyl oligopeptidase inhibition-induced growth arrest of human gastric cancer cells,2014

    • Author(s)
      Kanayo Suzuki, Minoru Sakaguchi, Satoshi Tanaka, Tadashi Yoshimoto , Masanori Takaoka,
    • Journal Title

      Biochim. Biophys. Res. Commun.

      Volume: 443 Pages: 91-96

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Prolyl oligopeptidase is a glyceraldehyde-3-phosphate dehydrogenase-binding protein that regulates genotoxic stress-induced cell death2013

    • Author(s)
      Matsuda T., Sakaguchi, M., Tanaka, S., Yoshimoto, T., Takaoka, M
    • Journal Title

      Int. J. Biochem. Cell Bio.

      Volume: 45 Pages: 850-857

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] 歯周病菌のプロリルトリペプチジルペプチダーゼを阻害する米ぬか由来タンパク質2015

    • Author(s)
      ○安形博菜1, 西村仁1,中嶋義隆1, 伊藤潔 2, 芳本忠
    • Organizer
      生物工学会
    • Place of Presentation
      鹿児島
    • Year and Date
      2015-10-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] A NEW INHIBITOR FROM RICE FOR PROLYL TRIPEPTIDYL PEPTIDASE FROM PORPHYROMONAS GINGIVALIS2015

    • Author(s)
      Hirona Agata, Hitoshi Nishimura, Yoshitaka Nakajima, Kiyoshi Ito, and Tadashi Yoshimoto
    • Organizer
      Internationnal Proteolysis Society
    • Place of Presentation
      マレーシア
    • Year and Date
      2015-10-05
    • Related Report
      2015 Annual Research Report
  • [Presentation] 米ぬか由来の歯周病菌プロリルトリペプチジルペプチダーゼ阻害タンパク質2015

    • Author(s)
      安形博菜、西村仁、中嶋義隆、伊藤潔、芳本忠
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      岡山大学
    • Year and Date
      2015-03-27
    • Related Report
      2014 Research-status Report
  • [Presentation] エノキタケ抽出物の歯周病菌由来プロリルトリペプチジルペプチダーゼ阻害作用2014

    • Author(s)
      安形博菜, 中嶋義隆, 伊藤潔、 芳本忠
    • Organizer
      日本生物工学会大会
    • Place of Presentation
      札幌コンベンション
    • Year and Date
      2014-08-09
    • Related Report
      2014 Research-status Report
  • [Book] 日本の結晶学(Ⅱ) その輝かしい発展2014

    • Author(s)
      芳本忠
    • Total Pages
      485
    • Publisher
      日本結晶学会
    • Related Report
      2014 Research-status Report
  • [Patent(Industrial Property Rights)] プロリルトリペプチジルペプチダーゼ阻害剤2015

    • Inventor(s)
      芳本 忠、中嶋 義隆、西村 仁、伊藤 潔、安形 博菜
    • Industrial Property Rights Holder
      学校法人 常翔学園
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2015-141260
    • Filing Date
      2015-07-15
    • Related Report
      2015 Annual Research Report

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Published: 2014-07-25   Modified: 2019-07-29  

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