New inhibitor for proline specific peptidases which cause several

• Project/Area Number: 25450138
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Setsunan University
• Principal Investigator: Yoshimoto Tadashi 摂南大学, 理工学部, 教授 (60088870)
• Co-Investigator(Kenkyū-buntansha): YONEYAMA Masanori 摂南大学, 薬学部, 講師 (00411710)
• Co-Investigator(Renkei-kenkyūsha): NAKAJIMA Yoshitaka 摂南大学, 理工学部, 教授 (80372770)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 歯周病 / 阻害剤 / プロリン / 米ぬか / プロリルトリペプチジルペプチダーゼ / ペプチダーゼ / 糖尿病

Outline of Final Research Achievements

Prolyl tripeptidyl peptidase (PTP) from P. gingivalis is a key enzyme of periodontitis. After screening of natural products, rice bran had high inhibitory activity for PTP. The inhibitor protein (PTPI) was purified from rice bran. By MALDI-TOF MS and database of rice, gene Os05g0323900 was identified as the PTP inhibitor. The protein was expressed as fuson protein with thioredoxin, rPTPI-Trx, in E. coli.,and crystallized by the sitting drop vapor diffusion method

Research Products

• [Journal Article] Prolyl oligopeptidase inhibition-induced growth arrest of human gastric cancer cells, (2014)
  • Author(s): Kanayo Suzuki, Minoru Sakaguchi, Satoshi Tanaka, Tadashi Yoshimoto , Masanori Takaoka,
  • Journal Title: Biochim. Biophys. Res. Commun. Volume: 443 Pages: 91-96
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Prolyl oligopeptidase is a glyceraldehyde-3-phosphate dehydrogenase-binding protein that regulates genotoxic stress-induced cell death (2013)
  • Author(s): Matsuda T., Sakaguchi, M., Tanaka, S., Yoshimoto, T., Takaoka, M
  • Journal Title: Int. J. Biochem. Cell Bio. Volume: 45 Pages: 850-857
  • Peer Reviewed
• [Presentation] 歯周病菌のプロリルトリペプチジルペプチダーゼを阻害する米ぬか由来タンパク質 (2015)
  • Author(s): ○安形博菜1, 西村仁1,中嶋義隆1, 伊藤潔 2, 芳本忠
  • Organizer: 生物工学会
  • Place of Presentation: 鹿児島
  • Year and Date: 2015-10-26
• [Presentation] A NEW INHIBITOR FROM RICE FOR PROLYL TRIPEPTIDYL PEPTIDASE FROM PORPHYROMONAS GINGIVALIS (2015)
  • Author(s): Hirona Agata, Hitoshi Nishimura, Yoshitaka Nakajima, Kiyoshi Ito, and Tadashi Yoshimoto
  • Organizer: Internationnal Proteolysis Society
  • Place of Presentation: マレーシア
  • Year and Date: 2015-10-05
• [Presentation] 米ぬか由来の歯周病菌プロリルトリペプチジルペプチダーゼ阻害タンパク質 (2015)
  • Author(s): 安形博菜、西村仁、中嶋義隆、伊藤潔、芳本忠
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 岡山大学
  • Year and Date: 2015-03-27
• [Presentation] エノキタケ抽出物の歯周病菌由来プロリルトリペプチジルペプチダーゼ阻害作用 (2014)
  • Author(s): 安形博菜, 中嶋義隆, 伊藤潔、 芳本忠
  • Organizer: 日本生物工学会大会
  • Place of Presentation: 札幌コンベンション
  • Year and Date: 2014-08-09
• [Book] 日本の結晶学（Ⅱ） その輝かしい発展 (2014)
  • Author(s): 芳本忠
  • Total Pages: 485
  • Publisher: 日本結晶学会
• [Patent(Industrial Property Rights)] プロリルトリペプチジルペプチダーゼ阻害剤 (2015)
  • Inventor(s): 芳本 忠、中嶋 義隆、西村 仁、伊藤 潔、安形 博菜
  • Industrial Property Rights Holder: 学校法人 常翔学園
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2015-141260
  • Filing Date: 2015-07-15