New inhibitor for proline specific peptidases which cause several
| Project/Area Number |
25450138
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Setsunan University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YONEYAMA Masanori 摂南大学, 薬学部, 講師 (00411710)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKAJIMA Yoshitaka 摂南大学, 理工学部, 教授 (80372770)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 歯周病 / 阻害剤 / プロリン / 米ぬか / プロリルトリペプチジルペプチダーゼ / ペプチダーゼ / 糖尿病 |
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| Outline of Final Research Achievements |
Prolyl tripeptidyl peptidase (PTP) from P. gingivalis is a key enzyme of periodontitis. After screening of natural products, rice bran had high inhibitory activity for PTP. The inhibitor protein (PTPI) was purified from rice bran. By MALDI-TOF MS and database of rice, gene Os05g0323900 was identified as the PTP inhibitor. The protein was expressed as fuson protein with thioredoxin, rPTPI-Trx, in E. coli.,and crystallized by the sitting drop vapor diffusion method
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Report
(4 results)
Research Products
(8 results)
