Elucidation of jasmonate-independent signal transduction pathways leading to phytoalexin production in rice

• Project/Area Number: 25450157
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bioorganic chemistry
• Research Institution: Teikyo University
• Principal Investigator: YAMANE Hisakazu 帝京大学, 理工学部, 教授 (80090520)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: イネ / 病害抵抗性 / ファイトアレキシン / ジャスモン酸 / サイトカイニン / エリシター / シグナル伝達 / ゲノム編集 / ジャスモン酸非依存性シグナル伝達経路 / ジャスモン酸欠損変異体

Outline of Final Research Achievements

Phytoalexins are antifungal secondary metabolites produced in response to pathogen infection in higher plants. It has been shown that signal transduction pathways leading to phytoalexin production are regulated by jasmonate (JA)-dependent and JA-independent pathways in rice. In this study, we suggested that the JA-independent pathway functions in the phytoalexin production induced by several abiotic stresses as well as pathogen infection, and that cytokinin functions as a second signal in the JA-independent pathway. In addition, we found cross-talk between JA and CK in the regulation of phytoalexin

Research Products

• [Journal Article] DITERPENOID PHYTOALEXIN FACTOR, a bHLH Transcription Factor, Plays a Central Role in the Biosynthesis of Diterpenoid Phytoalexins in Rice (2015)
  • Author(s): Yamamura C, Mizutani E, Okada K, Nakagawa H, Fukushima S, Tanaka A, Maeda S, Kamakura T, Yamane H, Takatsuji H, and Mori M
  • Journal Title: Plant J Volume: 84 Issue: 6 Pages: 1100-1113
  • DOI: 10.1111/tpj.13065
  • Peer Reviewed
• [Journal Article] OsJAR1 Contributes Mainly to Biosynthesis of the Stress-Induced Jasmonoyl-Isoleucine Involved in Defense Responses in Rice (2013)
  • Author(s): Shimizu T, Miyamoto K, Miyamoto K, Minami E, Nishizawa Y, Iino M, Nojiri N, Yamane H, Okada K
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 77 Issue: 7 Pages: 1556-1564
  • DOI: 10.1271/bbb.130272
  • NAID: 10031190468
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Presentation] イネの転写因子DPFによる根でのジテルペン型ファイトアレキシン生合成遺伝子及び共発現遺伝子の転写制御機構の解析 (2016)
  • Author(s): 山村千紘、水谷恵美、田中惇訓、福島説子、岡田憲典、鎌倉高志、山根久和、高辻博志、森 昌樹
  • Organizer: 日本農芸化学会2016年度大会
  • Place of Presentation: 札幌コンベンションセンター(北海道札幌市）
  • Year and Date: 2016-03-28
• [Presentation] Biosynthesis of phytoalexin biosynthesis and its regulatory mechanism in rice (2015)
  • Author(s): Hisakazu Yamane, Kazunori Okada, Masaki Mori and Tomonobu Toyomasu
  • Organizer: The 3rd Seminar on Biotechnology, Graduate School of Universitas Gadjah Mada Graduate Program in Biotechnology
  • Place of Presentation: Yogyakarta (Indonesia)
  • Year and Date: 2015-10-31
  • Invited
• [Presentation] イネの転写因子DPFはジテルペン型ファイトアレキシン生合成において中心的な役割を果たしている (2015)
  • Author(s): 山村千紘、水谷恵美、岡田憲典、鎌倉高志、山根久和、高辻博志、森 昌樹
  • Organizer: 第50回植物化学調節学会
  • Place of Presentation: 東京大学農学部（東京都文京区）
  • Year and Date: 2015-10-25
• [Presentation] イネDiterpene Phytoalexin Factorのジャスモン酸誘導発現を担う新規転写因子の探索 (2015)
  • Author(s): 堤 涼、宮本皓司、根元圭一郎、澤崎達也、森 昌樹、山根久和、野尻秀昭、岡田憲典
  • Organizer: 第50回植物化学調節学会
  • Place of Presentation: 東京大学農学部（東京都文京区）
  • Year and Date: 2015-10-25
• [Presentation] イネにおけるUV照射によるファイトアレキシン生産誘導機構へのジャスモン酸の関与 (2015)
  • Author(s): 宮本皓司、遠田勇海、岡田利樹、佐藤由実子、渡辺航平、酒澤智子、湯本絵美、柴田恭美、朝比奈雅志、横田孝雄、飯野盛利、岡田憲典、山根久和
  • Organizer: 日本農芸化学会2015年度大会
  • Place of Presentation: 岡山大学津島キャンパス
  • Year and Date: 2015-03-28