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Development of system for synthesis of Py-Im polyamide causing useful phenotypes to plants

Research Project

Project/Area Number 25450158
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Bioorganic chemistry
Research InstitutionKanto Gakuin University

Principal Investigator

Kondou Youichi  関東学院大学, 理工学部, 准教授 (00391954)

Co-Investigator(Kenkyū-buntansha) Mitsuda Nobutaka  産業技術総合研究所, その他部局等, 研究員 (80450667)
Co-Investigator(Renkei-kenkyūsha) Iida Hirokazu  関東学院大学, 理工学部, 准教授 (10335797)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
KeywordsPy-Imポリアミド / 遺伝子組換え / 転写因子 / シロイヌナズナ / 遺伝子発現制御 / CRES-T
Outline of Final Research Achievements

We have been constructed novel technique, in which a plant can acquire useful phenotypes by a chemical compound, pyrrole-imidazole (Py-Im) polyamide, which may bind to any nucleotide sequences. In order to construct this technique, we selected an Arabidopsis transcription factor, NST3, of which suppress cause inhibition of cell wall synthesis, and synthesized a compound, of which can bind to NST3 target sequence in MYB46 promoter. By using yeast-one hybrid system, the interaction between synthesized compound and the MYB46 promoter was verified. So we checked the effectiveness of the compound on the Arabidopsis plant. When plants were planted in the compound-containing medium, we observed the growth retardation. The transcriptome analysis suggested that growth retardation was caused by decreasing of expression levels of many genes including these related with synthesis of cell wall. This study is first possibility of regulation of gene expression in plant by using Py-Im polyamide.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (6 results)

All 2016 2015 2014

All Presentation (6 results)

  • [Presentation] Investigation of the effectiveness of neurodegenerative disease therapeutic agent, 4-PBA, on root hair2016

    • Author(s)
      Takahiro Sato, Shouta Hirose, Yuusuke Akatsu, Hirokazu Iida, Youichi Kondou
    • Organizer
      第57回日本植物生理学会年会
    • Place of Presentation
      岩手大学上田キャンパス
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] Establishment of a novel system for the isolation of useful genes functioning in most land plants using Marchantia polymorpha and Arabidopsis thaliana2016

    • Author(s)
      Takuya Terasawa, Ryota Nihei , Miyuki Kobayashi, Takeshi Yoshizumi, Minami Matsui, Youichi Kondou
    • Organizer
      第57回日本植物生理学会年会
    • Place of Presentation
      岩手大学上田キャンパス
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] 4-PBA及びその類似体添加による植物の高温ストレス耐性向上の検討2015

    • Author(s)
      古川 陽介、廣瀬 翔太、佐藤 貴大、赤津 悠輔、名取 将、水上 和史、髙橋 希恵、飯田 博一、近藤 陽一
    • Organizer
      2015年度関東学院大学理工/建築・環境学会研究発表講演会
    • Place of Presentation
      関東学院大学金沢八景キャンパス
    • Year and Date
      2015-11-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] 植物の有用遺伝子を探索する新たなシステムの確立2015

    • Author(s)
      寺澤 拓弥、小林 美由希、二瓶 遼太、宮城 祐太、西浜 竜一、石崎 公庸、河内 孝之、近藤 陽一
    • Organizer
      2015年度関東学院大学理工/建築・環境学会研究発表講演会
    • Place of Presentation
      関東学院大学金沢八景キャンパス
    • Year and Date
      2015-11-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] 植物に有用形質を付与する化合物の合成2014

    • Author(s)
      中田周斗、石渡周平、鈴木優衣、小糸喜来美、井上卓也、大髙霞、飯田博一、近藤陽一
    • Organizer
      2014年度関東学院大学工学部研究発表講演会
    • Place of Presentation
      関東学院大学
    • Year and Date
      2014-11-18
    • Related Report
      2014 Research-status Report
  • [Presentation] Py-Imポリアミド結合判定系の開発2014

    • Author(s)
      中田周斗、小糸喜来美、鈴木優衣、飯田博一、近藤陽一
    • Organizer
      第55回植物生理学会年会
    • Place of Presentation
      富山大学
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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