| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Outline of Final Research Achievements |
Some sulfur-containing compounds in foods have known to change and/or protein disulfide bond(s) by the oxidative modification of cysteine residues of intracellular specific protein(s). In this study, to understand the molecular mechanisms of physiological effects by diallyl trisulfide (DATS) and the other sulfur-containing compounds, intracellular target proteins contributing to the expression of physiological function by DATS were explored by the detection of the protein disulfide bond formation with the based on Redox two-dimensional-PAGE (Redox 2D-PAGE). As a result, four proteins as intracellular target proteins of DATS were detected with Redox 2D-PAGE and identified by LC-MS/MS, successfully. The disulfide bonds should be formed within these proteins by treatment of DATS, and the conformations of these proteins were changed. These specific and chemical reaction caused by DATS were thought to be the expression of physiological functions of DATS.
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