Precise analysis of pseudovitamin B12 compounds in food and effect of these compounds on vitamin B12 status of living cells

• Project/Area Number: 25450168
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Food science
• Research Institution: Tottori University
• Principal Investigator: Watanabe Fumio 鳥取大学, 農学部, 教授 (30210941)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: ビタミンB12 / 神経障害 / 欠乏症 / 擬似ビタミン / 貝類 / 藻類 / キノコ類 / 乳類 / ビタミンＢ１２ / 食品分析 / 疑似ビタミン / 食用貝類 / チーズ / 食用紅藻 / きのこ / 加工食品 / 食用藻類 / キノコ

Outline of Final Research Achievements

Foods derived from animals, namely fish, shellfish, and milk, are considered major dietary sources of vitamin B12 (B12). When food corrinoids were characterized using liquid chromatography-electrospray ionization/tandem mass spectrometry, certain food items (edible cyanobacteria and snails) contained pseduo B12 inactive in humans. Intrinsic factor involved in the gastrointestinal absorption of B12 preferentially bind B12 in the presence of pseudoB12 under physiological conditions. PseudoB12 did not function as cofactors of B12-depedent enzymes of COS7 cultured cells. These results suggest that pseudoB12 does not have the ability to act as a B12 antagonist in mammals.

Research Products

• [Journal Article] Determination and characterization of corrinoid compounds in truffle (Tuber spp.) and shoro (Rhizopogon rubescens) fruiting bodies (2015)
  • Author(s): Teng, F., Bito, T., Takenaka, S., Yabuta, Y., Shimomura, N., Watanabe, F.
  • Journal Title: Mushroom Science and Biotechnology Volume: 22 Pages: 159-164
  • NAID: 110009923642
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Occurrence of biologically inactive corrinoid compounds in canned edible apple snail (escargots) (2015)
  • Author(s): Teng, F., Tanioka, Y., Bito, T., Takenaka, S., Yabuta, Y., Watanabe, F.
  • Journal Title: Food and Nutrition Science Volume: 6 Issue: 12 Pages: 1071-1077
  • DOI: 10.4236/fns.2015.612111
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Determination and chacterization of vitamin B12 compounds in edible sea snails, ivory shell Babylonia japonica and turban shell Turdo Batillus cornutus (2015)
  • Author(s): Teng, F., Tanioka, Y., Hamaguchi, N., Bito, T., Takenaka, S., Yabuta, Y., Watanabe, F.
  • Journal Title: Fisheries Science Volume: 81 Issue: 6 Pages: 1105-1111
  • DOI: 10.1007/s12562-015-0920-5
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Determination and characterization of corrinoid compounds in truffe (Tuber spp.) and shoro (Rhizopogon rubescens) fruiting bodies (2015)
  • Author(s): Teng, F., Bito, T., Takenaka, S., Yabuta, Y., Shimomura, N., and Watanabe, F.
  • Journal Title: Mushroom Science and Biotechnology Volume: 22 Pages: 159-164
  • Peer Reviewed
• [Journal Article] Dodecylamine derivative of hydroxocobalamin acts as a potent inhibitor of cobalamin-dependent methionine synthase in mammalian cultured COS-7 cells (2014)
  • Author(s): Bito, T., Yasui, M., Iwaki, T., Yabuta, Y., Ichiyanagi, T., Yamaji, R., Nakano, Y., Inui, H., and Watanabe, F.
  • Journal Title: Food and Nutrition Science Volume: 5 Pages: 1311-1318
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Determination of vitamin B12 in Chinese black tea leaves (2014)
  • Author(s): Teng, F., Bito, T., Takenaka, S., Yabuta, Y., and Watanabe, F.
  • Journal Title: Food and Nutrition Science Volume: 5 Issue: 14 Pages: 1319-1325
  • DOI: 10.4236/fns.2014.514144
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Characterization of vitamin B12 compounds in the fruiting bodies of shiitake mushroom (Lentinula edodes) and bed logs after fruiting of the mushroom (2014)
  • Author(s): Bito, T., Teng, F., Ohishi, N., Takenaka, S., Miyamoto, E., Sakuno, E., Terashima, K., Yabuta, Y., and Watanabe, F.
  • Journal Title: Mycoscience Volume: 55 Issue: 6 Pages: 462-468
  • DOI: 10.1016/j.myc.2014.01.008
  • Peer Reviewed
• [Journal Article] Identification of vitamin B12 and pseudovitamin B12 from various edible shellfish using liquid chromatography-electrospray ionization/tandem mass spectrometry (2014)
  • Author(s): Taninoka, Y., Takenaka, S., Furusho, T., Yabuta, Y., Nakano, Y., and Watanabe, F.
  • Journal Title: Fisheries Science Volume: 80 Issue: 5 Pages: 1065-1071
  • DOI: 10.1007/s12562-014-0787-x
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Vitamin B12-containing plant food sources for vegetarians (2014)
  • Author(s): Watanabe, F., Yabuta, Y., Bito, T. and Teng, F.
  • Journal Title: Nutrients Volume: 6 Issue: 5 Pages: 1861-1873
  • DOI: 10.3390/nu6051861
  • Peer Reviewed / Open Access
• [Journal Article] Vitamin B12[c-lactone], a biologically inactive corrinoid compound, occurs in cultured and dried lion’s mane mushroom (Hericium erinaceus) fruiting bodies (2014)
  • Author(s): Teng, F., Bito, T., Takanaka, S., Yabuta, Y. and Watanabe, F.
  • Journal Title: Journal of Agricultural and Food Chemistry Volume: 62 Issue: 7 Pages: 1726-1732
  • DOI: 10.1021/jf404463v
  • Peer Reviewed
• [Journal Article] Characterization of corrinoid compounds in the edible cyanobacterium Nostoc flagelliforme the hair vegetable (2014)
  • Author(s): Teng, F., Bito, T., Takenaka, S., Takenaka, H., Yamaguchi, Y., Yabuta, Y. and Watanabe, F.
  • Journal Title: Food and Nutritional Sciences Volume: 5 Issue: 04 Pages: 334-340
  • DOI: 10.4236/fns.2014.54040
  • Peer Reviewed
• [Journal Article] Characterization of vitamin B12 compounds from the brackish-water bivalve Corbicula japonica (2013)
  • Author(s): Ishihara, Y., Ueta, K., Bito, T., Takenaka, S., Yabuta, Y. and Watanabe, F.
  • Journal Title: Fisheries Science Volume: 79 Issue: 2 Pages: 321-326
  • DOI: 10.1007/s12562-013-0596-7
  • Peer Reviewed
• [Journal Article] Biologically active vitamin B12 compounds in foods for preventing deficiency among vegetarians and elderly subjects (2013)
  • Author(s): Watanabe, F., Yabuta, Y., Tanioka, Y. and Bito, T.
  • Journal Title: Journal of Agricultural and Food Chemistry Volume: 61 Issue: 28 Pages: 6769-6775
  • DOI: 10.1021/jf401545z
  • Peer Reviewed
• [Presentation] ChlorellaにおけるビタミンB12の生理機能 (2015)
  • Author(s): 渡辺文雄・美藤友博・美藤麻里子・多湖一憲・浅井悠亮・薮田行哲・竹中重雄・大西真人・溝口亨
  • Organizer: 日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2015-12-01
• [Presentation] Characterization of vitamin B12 compounds in century eggs (Pidan) (2015)
  • Author(s): Teng, F., Takenaka, S., Yabuta, Y., Watanabe F.
  • Organizer: International Symposium on Agricultural, Food, Environmental and Life Sciences in Asia, 2015
  • Place of Presentation: 倉吉未来中心
  • Year and Date: 2015-11-04
• [Presentation] クロレラにおけるビタミンB12の生理機能について (2015)
  • Author(s): 美藤友博・美藤麻里子・浅井悠亮・多湖一憲・薮田行哲・竹中重雄・大西真人・溝口亨・渡邉文雄
  • Organizer: 日本栄養食糧学会中国・四国支部大会
  • Place of Presentation: 広島女学院大学
  • Year and Date: 2015-10-31
• [Presentation] 中華食材に含まれるビタミンB12化合物の特性 (2015)
  • Author(s): 滕飛・竹中重雄・薮田行哲・渡邉文雄
  • Organizer: 日本ビタミン学会大会
  • Place of Presentation: 奈良県新公会堂
  • Year and Date: 2015-06-05
• [Presentation] トリュフ及び松露に含まれるビタミンＢ12化合物の特性 (2014)
  • Author(s): 滕飛・竹中重雄・藪田行哲・霜村典宏・渡邉文雄
  • Organizer: 日本農芸化学会中四国支部
  • Place of Presentation: 徳島市（徳島大学・常三島キャンパス）
  • Year and Date: 2014-09-26 – 2014-09-27
• [Presentation] 栄養補助食品のクロレラ錠剤に含まれるビタミンＢ12化合物の精密分析 (2014)
  • Author(s): 浅井悠亮・美藤友博・多湖一憲・藪田行哲・竹中重雄・溝口亨・渡邉文雄
  • Organizer: 日本農芸化学会中四国支部
  • Place of Presentation: 徳島市（徳島大学・常三島キャンパス）
  • Year and Date: 2014-09-26 – 2014-09-27
• [Presentation] 塩素系殺菌剤がビタミンＢ12に及ぼす影響 (2014)
  • Author(s): 上田央・馬場康弘・藪田行哲・渡邉文雄
  • Organizer: 日本農芸化学会中四国支部
  • Place of Presentation: 徳島市（徳島大学・常三島キャンパス）
  • Year and Date: 2014-09-26 – 2014-09-27
• [Presentation] エスカルゴ水煮缶詰に含まれるビタミンB12化合物の特性 (2014)
  • Author(s): 膝飛・美藤友博・谷岡由梨・竹中重雄・薮田行哲・渡邉文雄
  • Organizer: 日本ビタミン学会
  • Place of Presentation: 姫路市(姫路商工会議所)
  • Year and Date: 2014-06-13 – 2014-06-14
• [Presentation] 熟成型チーズ類に含まれるビタミンＢ12化合物の特性 (2014)
  • Author(s): 谷岡由梨・竹中重雄・古庄律・藪田行哲・中野長久・渡邉文雄
  • Organizer: 日本ビタミン学会
  • Place of Presentation: 姫路市(姫路商工会議所)
  • Year and Date: 2014-06-13 – 2014-06-14
• [Presentation] 食用巻貝に含まれるビタミンB12化合物の特性 (2014)
  • Author(s): 膝飛・濱口菜摘・美藤友博・谷岡由梨・竹中重雄・薮田行哲・渡邉文雄
  • Organizer: 日本栄養・食糧学会
  • Place of Presentation: 北海道 (酪農学園大学)
  • Year and Date: 2014-05-30 – 2014-06-01
• [Presentation] 栄養補助食品のクロレラ錠剤に含まれるビタミンB12化合物の特性 (2014)
  • Author(s): 美藤友博・多湖一憲・薮田行哲・竹中重雄・溝口亨・渡辺文雄
  • Organizer: 日本栄養・食糧学会
  • Place of Presentation: 北海道 (酪農学園大学)
  • Year and Date: 2014-05-30 – 2014-06-01
• [Presentation] 栄養補助食品のクロレラ錠剤に含まれるコリノイド化合物の分析 (2013)
  • Author(s): 多湖一憲・藪田行哲・竹中重雄・溝口 亨・渡辺文雄
  • Organizer: 日本農芸化学会・日本ビタミン学会合同広島大会
  • Place of Presentation: 広島（広島県立大学）
• [Presentation] 髪菜に含まれるビタミンＢ12化合物の特性 (2013)
  • Author(s): 滕飛・竹中重雄・竹中裕行・藪田行哲・渡辺文雄
  • Organizer: 日本農芸化学会・日本ビタミン学会合同広島大会
  • Place of Presentation: 広島（広島県立大学）
• [Presentation] シイタケ（Lentinula edodes）に含まれるビタミンＢ12化合物の特性とその起源 (2013)
  • Author(s): 渡辺文雄・大石典治・美藤友博・宮本恵美・竹中重雄・作野えみ・藪田行哲
  • Organizer: 日本ビタミン学会第65回大会
  • Place of Presentation: 東京（一橋大学一橋講堂）