• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of genetic markers closely-linked to a male sterility locus in Cryptomeria japonica

Research Project

Project/Area Number 25450223
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Forest science
Research InstitutionForestry and Forest Products Research Institute

Principal Investigator

Ueno Saneyoshi  国立研究開発法人 森林総合研究所, 森林遺伝研究領域, チーム長 (40414479)

Co-Investigator(Kenkyū-buntansha) FUTAMURA Norihiro  国立研究開発法人森林総合研究所, 生物工学研究領域, 室長 (80343804)
Co-Investigator(Renkei-kenkyūsha) MORIGUCHI Yoshinari  新潟大学, 自然科学系, 助教 (60644804)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords遺伝マーカー / 針葉樹 / 表現型 / ゲノム / バイオインフォマティクス / 花粉症 / 形質 / スギ / 雄性不稔 / RNA-Seq / RAD-Seq / 遺伝的変異 / 連鎖解析
Outline of Final Research Achievements

Male sterility locus (ms1) in sugi (Cryptomeria japonica) was mapped using a back-cross family, where 85 fertile and 88 sterile individuals were included. SNP markers were developed from RNA-Seq and ddRAD-Seq approach. One of the markers developed in the present study was located at 0.6 cM apart from ms1. Phenotypes regarding to the ms1 was correctly estimated by the genotype of the marker for 99 % of offspring in the family.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (8 results)

All 2016 2015 2014 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Acknowledgement Compliant: 2 results,  Open Access: 1 results) Presentation (5 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Remarks (1 results)

  • [Journal Article] A high-density linkage map with 2,560 markers and its application for the localization of the male-sterile genes ms3 and ms4 in Cryptomeria japonica D. Don2016

    • Author(s)
      Yoshinari Moriguchi, Kentaro Uchiyama, Saneyoshi Ueno, Tokuko Ujino-Ihara, Asako Matsumoto, Junji Iwai, Daisuke Miyajima, Maki Saito, Masaya Sato, Yoshihiko Tsumura
    • Journal Title

      Tree Genetics and Genomes

      Volume: 12

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] A simple allele-specific PCR marker for identifying male-sterile trees: Towards DNA marker-assisted selection in the Cryptomeria japonica breeding program2014

    • Author(s)
      Moriguchi Y, Ueno S, Saito M, Higuchi Y, Miyajima D, Itoo S, Tsumura Y
    • Journal Title

      Molecular Breeding

      Volume: 10 Issue: 4 Pages: 1069-1077

    • DOI

      10.1007/s11295-014-0743-z

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] DNAマーカーを用いた無花粉スギのピラミッディング育種2016

    • Author(s)
      森口喜成、内山憲太郎、上野真義、松本麻子、伊原徳子、岩井淳治、戸塚聡子、津村義彦
    • Organizer
      第127回日本森林学会
    • Place of Presentation
      日本大学
    • Year and Date
      2016-03-29
    • Related Report
      2015 Annual Research Report
  • [Presentation] 樹木種のゲノムワイド解析に向けたマーカー整備2016

    • Author(s)
      内山憲太郎、加藤珠理、上野真義、鈴木節子、須貝杏子、松本麻子
    • Organizer
      第127回日本森林学会
    • Place of Presentation
      日本大学
    • Year and Date
      2016-03-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] Sugi genome resources2015

    • Author(s)
      Saneyoshi Ueno, Yoshinari Moriguchi, Kentaro Uchiyama, Tokuko Ujino-Ihara, Norihiro Futamura, Asako Matsumoto, Yoshihiko Tsumura
    • Organizer
      ProCoGen final open conference
    • Place of Presentation
      Orleans, France
    • Year and Date
      2015-11-29
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 森林樹木種における効率的なゲノムワイドマーカーの開発2015

    • Author(s)
      内山憲太郎、加藤珠理、上野真義、松本麻子
    • Organizer
      森林遺伝育種学会第4回大会
    • Place of Presentation
      東京大学
    • Year and Date
      2015-11-06
    • Related Report
      2015 Annual Research Report
  • [Presentation] スギ雄性不稔遺伝子(ms-1~ ms-4)の連鎖地図上の位置の特定2015

    • Author(s)
      森口喜成,内山憲太郎,上野真義,伊原徳子,松本麻子,斎藤真己,岩井淳治,宮嶋大介,樋口有未,伊藤信治,佐藤雅哉,津村義彦
    • Organizer
      日本育種学会第128回講演会
    • Place of Presentation
      新潟大学
    • Year and Date
      2015-09-12
    • Related Report
      2015 Annual Research Report
  • [Remarks] Sugi Genome Database

    • URL

      https://www.ffpri.affrc.go.jp/labs/cjgenome/index.html

    • Related Report
      2015 Annual Research Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi