Development of genetic markers closely-linked to a male sterility locus in Cryptomeria japonica

• Project/Area Number: 25450223
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Forest science
• Research Institution: Forestry and Forest Products Research Institute
• Principal Investigator: Ueno Saneyoshi 国立研究開発法人 森林総合研究所, 森林遺伝研究領域, チーム長 (40414479)
• Co-Investigator(Kenkyū-buntansha): FUTAMURA Norihiro 国立研究開発法人森林総合研究所, 生物工学研究領域, 室長 (80343804)
• Co-Investigator(Renkei-kenkyūsha): MORIGUCHI Yoshinari 新潟大学, 自然科学系, 助教 (60644804)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 遺伝マーカー / 針葉樹 / 表現型 / ゲノム / バイオインフォマティクス / 花粉症 / 形質 / スギ / 雄性不稔 / RNA-Seq / RAD-Seq / 遺伝的変異 / 連鎖解析

Outline of Final Research Achievements

Male sterility locus (ms1) in sugi (Cryptomeria japonica) was mapped using a back-cross family, where 85 fertile and 88 sterile individuals were included. SNP markers were developed from RNA-Seq and ddRAD-Seq approach. One of the markers developed in the present study was located at 0.6 cM apart from ms1. Phenotypes regarding to the ms1 was correctly estimated by the genotype of the marker for 99 % of offspring in the family.

Research Products

• [Journal Article] A high-density linkage map with 2,560 markers and its application for the localization of the male-sterile genes ms3 and ms4 in Cryptomeria japonica D. Don (2016)
  • Author(s): Yoshinari Moriguchi, Kentaro Uchiyama, Saneyoshi Ueno, Tokuko Ujino-Ihara, Asako Matsumoto, Junji Iwai, Daisuke Miyajima, Maki Saito, Masaya Sato, Yoshihiko Tsumura
  • Journal Title: Tree Genetics and Genomes Volume: 12
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A simple allele-specific PCR marker for identifying male-sterile trees: Towards DNA marker-assisted selection in the Cryptomeria japonica breeding program (2014)
  • Author(s): Moriguchi Y, Ueno S, Saito M, Higuchi Y, Miyajima D, Itoo S, Tsumura Y
  • Journal Title: Molecular Breeding Volume: 10 Issue: 4 Pages: 1069-1077
  • DOI: 10.1007/s11295-014-0743-z
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] DNAマーカーを用いた無花粉スギのピラミッディング育種 (2016)
  • Author(s): 森口喜成、内山憲太郎、上野真義、松本麻子、伊原徳子、岩井淳治、戸塚聡子、津村義彦
  • Organizer: 第127回日本森林学会
  • Place of Presentation: 日本大学
  • Year and Date: 2016-03-29
• [Presentation] 樹木種のゲノムワイド解析に向けたマーカー整備 (2016)
  • Author(s): 内山憲太郎、加藤珠理、上野真義、鈴木節子、須貝杏子、松本麻子
  • Organizer: 第127回日本森林学会
  • Place of Presentation: 日本大学
  • Year and Date: 2016-03-28
• [Presentation] Sugi genome resources (2015)
  • Author(s): Saneyoshi Ueno, Yoshinari Moriguchi, Kentaro Uchiyama, Tokuko Ujino-Ihara, Norihiro Futamura, Asako Matsumoto, Yoshihiko Tsumura
  • Organizer: ProCoGen final open conference
  • Place of Presentation: Orleans, France
  • Year and Date: 2015-11-29
  • Int'l Joint Research / Invited
• [Presentation] 森林樹木種における効率的なゲノムワイドマーカーの開発 (2015)
  • Author(s): 内山憲太郎、加藤珠理、上野真義、松本麻子
  • Organizer: 森林遺伝育種学会第４回大会
  • Place of Presentation: 東京大学
  • Year and Date: 2015-11-06
• [Presentation] スギ雄性不稔遺伝子（ms-1～ ms-4）の連鎖地図上の位置の特定 (2015)
  • Author(s): 森口喜成，内山憲太郎，上野真義，伊原徳子，松本麻子，斎藤真己，岩井淳治，宮嶋大介，樋口有未，伊藤信治，佐藤雅哉，津村義彦
  • Organizer: 日本育種学会第128回講演会
  • Place of Presentation: 新潟大学
  • Year and Date: 2015-09-12
• [Remarks] Sugi Genome Database
  • URL: https://www.ffpri.affrc.go.jp/labs/cjgenome/index.html