Development of the method to identify the gene involved in fruiting body formation of Lentinula edodes

Research Project

Project/Area Number	25450245
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Wood science
Research Institution	University of Miyazaki
Principal Investigator	Meguro Sadatoshi 宮崎大学, 農学部, 教授 (50112321)
Co-Investigator(Kenkyū-buntansha)	KAMEI Ichiro 宮崎大学, 農学部, 教授 (90526526)
Project Period (FY)	2013-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000) Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000) Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000) Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords	シイタケ / 子実体形成 / RNAi / 形質転換 / 光受容体 / きのこ / プロトプラスト
Outline of Final Research Achievements	This study developed the transformation method with RNAi construct for knockdown of specific gene involved in the fruiting-body formation of edible mushroom Lentinula edodes. Photoreceptor gene A of L. edodes (Le.phrA) was cloned and 600 bp of coding region was used for RNAi construct ligated with the partial sequence of GUS gene. RNAi construct for Le. phrA was successfully introduced with selection marker vector into protoplast of L. edodes. Some transformants showed brown color mycelium, and that the growth was inhibited remarkably. This phenotype might be caused by RNAi knockdown of Le. phrA.