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Production of novel glycoproteins participating wood decay and application to degradation of biomass

Research Project

Project/Area Number 25450247
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Wood science
Research InstitutionKindai University

Principal Investigator

TANAKA Hiromi  近畿大学, 農学部, 教授 (30140338)

Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords木材分解 / 水酸化ラジカル / 糖タンパク質 / ピリジン補酵素 / 褐色腐朽菌 / 一電子酸化活性 / 白色腐朽菌 / タンパク質発現
Outline of Final Research Achievements

During wood degradation, white-rot, brown-rot, and soft-rot fungi produce glycoprotein that catalyzes a redox reaction between oxygen and electron donors to produce hydroxyl radicals, reduces Fe(III) to Fe(II), and strongly binds Fe(II). The continuous generation of hydroxyl radicals requires a constant supply of oxygen and an electron donor for the reduction of oxidized form of the glycoprotein to the reduced form. The extracellular pyridine coenzymes were detected in cultures of white-, brown-, and soft-rot fungi. Thus, these pyridine coenzymes could serve as electron donors for the production of hydroxyl radicals during wood degradation. Moreover, the culture method for brown-rot fungi was examined by determining ethylene generation which indicates one-electron oxidation activity.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (7 results)

All 2017 2015 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (6 results)

  • [Journal Article] Presence of Extracellular NAD+ and NADH in Cultures of Wood-Degrading Fungi2015

    • Author(s)
      Ryuta Kido, Midori Takeeda, Mitsuhiro Manabe, Yutaka Miyagawa, Shuji Itakura, and Hiromi Tanaka
    • Journal Title

      Biocontrol Sci.

      Volume: 20 Pages: 105-113

    • NAID

      130005085082

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 木材腐朽における微生物遷移と木材腐朽機構2017

    • Author(s)
      田中裕美
    • Organizer
      第67回日本木材学会大会
    • Place of Presentation
      九州大学(福岡県福岡市)
    • Year and Date
      2017-03-19
    • Related Report
      2016 Annual Research Report
  • [Presentation] 種々な木材生息菌の培養基中のピリジン補酵素の測定2015

    • Author(s)
      田中裕美、中桐亮太、福林貴仁、板倉修司
    • Organizer
      日本防菌防黴学会
    • Place of Presentation
      千里ライフサイエンスセンター(大阪)
    • Year and Date
      2015-09-01
    • Related Report
      2015 Research-status Report
  • [Presentation] Extracellular NAD+ and NADH by white-rot, brown-rot, and soft-rot fungi2015

    • Author(s)
      Ryuta Kido, Midori Takeeda, Mitsuhiro Manabe, Yutaka Miyagawa, Tatsuhiro Katashiba, Masayasu Yamauchi, Shuji Itakura, Hiromi Tanaka
    • Organizer
      国際シンポジウム(IAWPS2015)
    • Place of Presentation
      タワーホール船堀(東京都)
    • Year and Date
      2015-03-16
    • Related Report
      2014 Research-status Report
  • [Presentation] 軟腐朽菌Graphium sp.ラッカーゼの諸性質および生産性向上のための培養条件の検討

    • Author(s)
      田中裕美、山本貴義、神谷祐樹、熱川涌晃、板倉修司
    • Organizer
      日本木材保存協会第29回年次大会
    • Place of Presentation
      メルパルク東京(東京都)
    • Related Report
      2013 Research-status Report
  • [Presentation] 軟腐朽菌Graphium sp.ラッカーゼの分離・精製および物理化学的諸性質

    • Author(s)
      田中裕美、山本貴義、沼田崚太、板倉修司
    • Organizer
      日本防菌防黴学会第40回年次大会
    • Place of Presentation
      千里ライフサイエンスセンター(大阪府)
    • Related Report
      2013 Research-status Report
  • [Presentation] 白色腐朽菌Phanerochaete chrysosporiumの分泌する水酸化ラジカル生成物質遺伝子の発現系の構築

    • Author(s)
      城戸竜太、板倉修司、田中裕美
    • Organizer
      第64回日本木材学会大会
    • Place of Presentation
      愛媛大学(愛媛県)
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2020-01-20  

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