| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
A facultative anaerobic bacterivorous nanoflagellate was isolated from the oxic-anoxic interface of the meromictic Lake Suigetsu, Japan. We characterized the isolate using light and transmission electron microscopy and molecular phylogenetic analyses. The isolate displayed some characteristic features different from the known species in class Placididea. The 18S rDNA sequence was also phylogenetically distant from the clades of the known Placididae. Thus, we described the newly isolate as Suigetsumonas clinomigrationis gen. et sp. nov. Growth and grazing kinetics were studied in the strain using co-culture experiments with a single bacterium. The growth and bacterivorous characteristics showed that the isolate can grow at full depth of Lake Suigetsu throughout year, but may be limited by prey bacterial densities in natural environments. High abundance of S. clinomigrationis was detected from the interface of Lake Suigetsu by means of CARD-FISH with the species-specific probe.
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