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Response of the family Vibrionaceae in the intestinal tracts of fish subjected to the stressful conditions

Research Project

Project/Area Number 25450263
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Aquatic bioproduction science
Research InstitutionNihon University

Principal Investigator

SUGITA Haruo  日本大学, 生物資源科学部, 教授 (50139052)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords腸内細菌叢 / トラフグ / プロバイオティクス / ビブリオ科細菌 / 飼育密度 / 飼育温度 / 配合飼料 / qPCR / 腸内細菌 / Vibrio / 飼育ストレス / 日和見感染症 / 細菌叢 / 定量PCR / Vibrio科細菌 / 魚類 / 細菌群集 / クローンライブラィ / 養殖 / クローンライブラリィ
Outline of Final Research Achievements

(1) As a model system of intestinal contents of pufferfish (Takifugu rubripes), the slurry which was prepared by mixing the grinded feeds and intestinal contents of pufferfish was evaluated. However, the bacterial community in the slurry was remarkably different from that of feeds and intestinal contents, suggesting that this system needs to be improved for the study of intestinal microbiology of pufferfish.
(2) Pufferfish was reared in glass aquaria under the stressful conditions of fish density and temperatures, and the family Vibrionaceae in the intestinal tract of pufferfish was monitored. As a result, the trend that the family Vibrionaceae was enhanced to some extent as the fish density increased in the aquaria. This fact suggests that Vibrionaceae may proliferate rapidly under stressful conditions such as high density of rearing fish.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (27 results)

All 2016 2015 2014 2013 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (17 results) Book (3 results)

  • [Journal Article] Spawning ecology of Girella punctata and G. leonina (Perciformes: Girellidae) in the coastal waters of the Izu Peninsula, Japan2015

    • Author(s)
      S. Nakai, T. Higuchi, Y. Iino, S. Itoi, H. Sugita and N. Takai
    • Journal Title

      Coastal Marine Science

      Volume: 38 Pages: 12-20

    • NAID

      120005627714

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Toxic Takifugu pardalis eggs found in T. niphobles gut: implications for TTX accumulation in the pufferfish2015

    • Author(s)
      Itoi S, Kozaki A, Komori K, Tsunashima T, Noguchi S, Kawane M, Sugita H
    • Journal Title

      Toxicon

      Volume: 108 Pages: 141-146

    • DOI

      10.1016/j.toxicon.2015.10.009

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The complete mitochondrial genome of the gnomefish Scombrops boops (Teleostei, Perciformes, Scombropidae) from the Pacific Ocean off the Japanese Islands2015

    • Author(s)
      Tsunashima Tadasuke, Shiro Itoi, Koko Abe, Tomoyuki Takigawa, Satoshi Inoue, Takahiro Kozen, Naoto Ono, Shunsuke Noguchi, Shizuko Nakai, Noriyuki Takai, Ming-Chih Huang, Haruo Sugita
    • Journal Title

      Mitochondrial DNA

      Volume: - Issue: 1 Pages: 785-786

    • DOI

      10.3109/19401736.2014.987242

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Variations in bile tolerance among Lactococcus lactis strains derived from different sources2014

    • Author(s)
      S. Takanashi, A. Miura, K. Abe, J. Uchida, S. Itoi and H. Sugita
    • Journal Title

      Folia Microbiologica

      Volume: 59 Issue: 4 Pages: 289-293

    • DOI

      10.1007/s12223-013-0297-8

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Habitat use of the gnomefishes, Scombrops boops and Scombrops gilberti, in the northwestern Pacific Ocean, in relation to reproductive strategy2014

    • Author(s)
      N. Takai, Y. Kozuka, T. Tanabe, Y. Sagara, M. Ichihashi, S. Nakai, M. Suzuki, N. Mano, S. Itoi, K. Asahina, T. Kojima, H. Sugita
    • Journal Title

      Aquatic Biology

      Volume: 21 Issue: 2 Pages: 109-120

    • DOI

      10.3354/ab00575

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The clam Meretrix lamarckii (Bivalvia: Veneridae) is a rich repository of marine lactic acid bacterial strains2014

    • Author(s)
      S. Itoi, J. Uchida, S. Takanashi, T. Narita, K. Abe, S. Naya and H. Sugita
    • Journal Title

      Annals of Microbiology

      Volume: 64 Issue: 3 Pages: 1267-1274

    • DOI

      10.1007/s13213-013-0771-1

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Predominant intestinal bacteria of the pufferfish (Takifugu rubripes Temminck & Schlegel, 1850) reared in an indoor tank as determined by the clone library analysis and culture method2013

    • Author(s)
      C. H. Chen, Y. Tanaka, Y. Komiya, S. Itoi and H. Sugita
    • Journal Title

      Journal of Applied Ichthyology

      Volume: 29 Issue: 6 Pages: 1374-1377

    • DOI

      10.1111/jai.12215

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] 分子生物学的手法による有毒ヒラムシPlanocera属の検出法の開発2016

    • Author(s)
      山田理子、綱島忠相、梶谷雄介、糸井史朗、杉田治男
    • Organizer
      日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京都港区)
    • Year and Date
      2016-03-29
    • Related Report
      2015 Annual Research Report
  • [Presentation] 培養条件下でのトラフグ腸内細菌叢に及ぼす配合飼料の影響2015

    • Author(s)
      南島諒輔、永田 務、鈴木健太、内藤吏美、糸井史朗、杉田治男
    • Organizer
      日本水産増殖学会大会
    • Place of Presentation
      東京海洋大学館山ステーション(千葉県館山市)
    • Year and Date
      2015-11-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] 九州南部海域で漁獲されたムツ属魚類の種同定2015

    • Author(s)
      望月裕香子、糸井史朗、増田育司、宍道弘敏、高井則之、中井静子、杉田治男
    • Organizer
      日本水産学会秋季大会
    • Place of Presentation
      東北大学(宮城県仙台市)
    • Year and Date
      2015-09-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] TTXを保有するヒラムシ類の系統学的地位および保有毒量の経年変化2015

    • Author(s)
      綱島忠相、山田理子、萩谷盛雄、梶谷雄介、糸井史朗、杉田治男
    • Organizer
      日本水産学会秋季大会
    • Place of Presentation
      東北大学(宮城県仙台市)
    • Year and Date
      2015-09-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] ムツ属魚類Scombros boopsおよびScombrops gilbertiのミトコンドリアゲノム全塩基配列の比較2015

    • Author(s)
      綱島忠相、糸井史朗、阿部江子、高井則之、中井静子、杉田治男
    • Organizer
      マリンバイオテクノロジー学会大会
    • Place of Presentation
      三重大学(三重県津市)
    • Year and Date
      2015-05-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] 多岐腸目ヒラムシ類におけるTTX保有種の探索2015

    • Author(s)
      綱島忠相, 伊澤心, 山田理子, 糸井史朗, 萩谷盛雄, 杉田治男
    • Organizer
      平成27年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京)
    • Year and Date
      2015-03-28
    • Related Report
      2014 Research-status Report
  • [Presentation] 16S rDNA-based metagenome analysis of microorganisms from Tilapia culture ponds in Thailand2014

    • Author(s)
      U. Watanabe, H. Takeyama, M. Ito, T. Aoki, M. Hattori, W. Suda, J. Hikima, M. Sakai, H. Sugita, J. Wongtavatachai, M. Lukkana, S. Jantrakajorn, S. Unajak, M. Rapeepat, C.F. Lo, H.C. Wang
    • Organizer
      平成26年度日本水産学会秋季大会
    • Place of Presentation
      九州大学(福岡)
    • Year and Date
      2014-09-21
    • Related Report
      2014 Research-status Report
  • [Presentation] クルマエビ Marsupenaeus japonicusにおいて二本鎖RNAにより誘導される遺伝子について2014

    • Author(s)
      小松真未, 糸井史朗, 杉田治男, 近藤秀裕, 廣野育夫
    • Organizer
      平成26年度日本水産学会秋季大会
    • Place of Presentation
      九州大学(福岡)
    • Year and Date
      2014-09-21
    • Related Report
      2014 Research-status Report
  • [Presentation] TTXを保有する多岐腸目ヒラムシ類の系統分類2014

    • Author(s)
      綱島忠相, 伊澤心, 山田理子,糸井史朗, 萩谷盛雄, 杉田治男
    • Organizer
      平成27年度日本水産学会秋季大会
    • Place of Presentation
      九州大学(福岡)
    • Year and Date
      2014-09-20
    • Related Report
      2014 Research-status Report
  • [Presentation] A molecular framework for the taxonomy of the marine turbellarian flatworms (Platyhelminthes, Polycladida)2014

    • Author(s)
      T. Tsunashima, M. Hagiya, N. Tsuyuki, S. Itoi and H. Sugita
    • Organizer
      The 10th Asia-Pacific Marine Biotechnology Conference (2014 APMBC)
    • Place of Presentation
      Academic Sinica, Taipei, Taiwan
    • Year and Date
      2014-05-06
    • Related Report
      2014 Research-status Report
  • [Presentation] Maternal TTX in the pufferfish bobies contribute to beneficial strategies for increasing the survival of egg and larvae2014

    • Author(s)
      S. Itoi, S. Yoshikawa, K. Asahina, M. Suzuki, K. Ishizuka, N. Takimoto, R. Mitsuoka, N. Yokoyama, A. Detake, C. Takayanagi, M. Eguchi, R. Tatsuno, M. Kawane, S. Kokubo, S. Takanashi, A. Miura, K. Suitoh, T. Takatani, O. Arakawa, Y. Sakakura and H. Sugita
    • Organizer
      The 10th Asia-Pacific Marine Biotechnology Conference (2014 APMBC)
    • Place of Presentation
      Academic Sinica, Taipei, Taiwan
    • Year and Date
      2014-05-06
    • Related Report
      2014 Research-status Report
  • [Presentation] ハマグリ腸内容物から分離された乳酸菌の特徴

    • Author(s)
      内田潤也、高梨志保里、成田知世、阿部江子、崎田祥、糸井史朗、杉田治男
    • Organizer
      第15回マリンバイオテクノロジー学会大会
    • Place of Presentation
      沖縄県市町村自治会館
    • Related Report
      2013 Research-status Report
  • [Presentation] トラフグ飼育水におけるビブリオ科細菌の動態

    • Author(s)
      相良和之、高橋佑典、南島諒輔、糸井史朗、杉田治男
    • Organizer
      日本水産増殖学会第12回大会
    • Place of Presentation
      鹿児島大学
    • Related Report
      2013 Research-status Report
  • [Presentation] 二枚貝のタイラギおよびタガイ腸内容物からの乳酸菌分離の試み

    • Author(s)
      内田潤也、崎田 祥、市川大介、糸井史朗、杉田治男
    • Organizer
      日本水産増殖学会第12回大会
    • Place of Presentation
      鹿児島大学
    • Related Report
      2013 Research-status Report
  • [Presentation] Isolation of lactic acid bacteria from the intestinal tract of bivalves

    • Author(s)
      J. Uchida, S. Sakita, D. Ichikawa, S. Takanashi, T. Narita, K. Abe,S. Itoi and H. Sugita
    • Organizer
      10th International Marine Biotechnology Conference
    • Place of Presentation
      Brisbane, Australia
    • Related Report
      2013 Research-status Report
  • [Presentation] 飼育トラフグの腸管内におけるVibrio科細菌の動態

    • Author(s)
      和之, 高橋佑典, 南島諒輔, 糸井史朗, 杉田治男
    • Organizer
      平成26年度日本水産学会春季大会
    • Place of Presentation
      北海道大学函館キャンパス
    • Related Report
      2013 Research-status Report
  • [Presentation] 淡水産二枚貝からの乳酸菌の分離およびその特性-海産二枚貝類由来株の表現形質との比較-

    • Author(s)
      内田潤也, 崎田洋, 市川大介, 小糸智子, 糸井史朗, 杉田治男
    • Organizer
      平成26年度日本水産学会春季大会
    • Place of Presentation
      北海道大学函館キャンパス
    • Related Report
      2013 Research-status Report
  • [Book] 増補改訂版・養殖の水と餌-陰の主役たち2014

    • Author(s)
      杉田治男(編著)
    • Total Pages
      200
    • Publisher
      恒星社厚生閣
    • Related Report
      2014 Research-status Report
  • [Book] 環境と微生物の事典(日本微生物生態学会編)2014

    • Author(s)
      杉田治男(共著)
    • Total Pages
      432
    • Publisher
      朝倉書店
    • Related Report
      2014 Research-status Report
  • [Book] 水族館と海の生き物たち2014

    • Author(s)
      杉田治男(編著)
    • Total Pages
      193
    • Publisher
      恒星社厚生閣
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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