Studies on syntrophic relationship among marine methane oxidizing microorganisms

• Project/Area Number: 25450265
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Aquatic bioproduction science
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: Takeuchi Mio 国立研究開発法人産業技術総合研究所, 生命工学領域, 主任研究員 (20357403)
• Co-Investigator(Renkei-kenkyūsha): IWASAKI Wataru 東京大学, 大学院理学系研究科(理学部), 准教授 (50545019)
• Project Period (FY): 2013-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: メタン / 共生 / ゲノム / 海洋細菌 / メタノトロフ / メチロトロフ / メタン酸化細菌 / 海底堆積物 / メタノール資化細菌 / 微生物間共生 / 環境微生物

Outline of Final Research Achievements

The intimate relationship between methane-oxidizing bacteria (methanotrophs) and methanol-oxidizing bacteria (methylotrophs) has been known for a long time. The co-existence with methylotrophs has been always explained in terms of cross-feeding. However, the real nature of the relationship remains unknown. In this study, we have isolated novel marine mwethanotroph, Methylocaldum marinum S8, and novel methylotroph, Methyloceanibacter caenitepidi Gela4, that syntrophically grow with M. marinum S8. We also determined complete genomes of the two strains in order to infer their interrelationship.

Research Products

• [Journal Article] Tepidicaulis marinus gen. nov., sp. nov., a novel marine bacterium reducing nitrate to nitrous oxide strictly under microaerobic conditions. (2015)
  • Author(s): Mio Takeuchi, Takao Yamagishi, Yoichi Kamagata, Kenshiro Oshima, Masahira Hattori, Taiki Katayama, Satoshi Hanada, Hideyuki Tamaki, Katsumi Marumo, Hiroto Maeda, Munetomo Nedachi, Wataru Iwasaki, Yuichi Suwa, and Susumu Sakata.
  • Journal Title: International Journal of Systematic and Evolutionary Microbiology, published online (2015) Volume: in press Issue: Pt_6 Pages: 1749-1754
  • DOI: 10.1099/ijs.0.000167
  • Peer Reviewed
• [Journal Article] Methyloceanibacter caenitepidi gen. nov., sp. nov., a novel facultatively methylotrophic bacterium isolated from marine sediments near the hydrothermal vent area (2014)
  • Author(s): Takeuchi, M., Katayama, T., Yamagishi, T., Hanada, S., Tamaki, H., Kamagata, Y., Oshima, K., Hattori, M., Marumo, K., Nedachi, M., Maeda, H., Suwa, Y., and Sakata, S.
  • Journal Title: Int J Syst Evol Microbiol Volume: 64 Issue: Pt_2 Pages: 462-468
  • DOI: 10.1099/ijs.0.053397-0
  • Peer Reviewed
• [Presentation] ゲノム解析・RNAseqによるメタン酸化細菌とメタノール資化性菌の共生機構の解明 (2017)
  • Author(s): 竹内美緒，尾崎 遼、平岡聡史、大島健志朗，服部正平，鎌形洋一，岩崎渉
  • Organizer: 環境微生物系学会合同大会
• [Presentation] Cross-feeding pathways from a methanotroph to a marine methylotroph, Methy1loceanibacter caenitep idi Gela4, under co-culture condition (2017)
  • Author(s): Mio Takeuchi, Haruka Ozaki, Satoshi Hiraoka, Yoichi Kamagata, Susumu Sakata, Wataru Iwasaki
  • Organizer: the 23rdInternational Symposium on Environmental Biogeochemistry (ISEB23)
  • Int'l Joint Research
• [Presentation] Novel marine moderately thermophilic microbes isolated from microbial consortium depends on methane
  • Author(s): Mio Takeuchi，Katsumi Marumo, Hiroto Maeda，Munetomo Nedachi，Kenshiro Oshima，Taiki Katayama，Takao Yamagishi，Wataru Iwasaki，Yoichi Kamagata，Satoshi Hanada，Hideyuki Tamaki，Masahira Hattori，Yuichi Suwa，Susumu Sakata
  • Organizer: The 5th Taiwan-Korea-Japan International Symposium on Microbial Ecology
  • Place of Presentation: National Central University, Jhongli,Taiwan
• [Presentation] 鹿児島大学鹿児島湾海底堆積物中に生息するメタンを基盤とする新規中温性微生物群の生理学的・遺伝学的特徴の解明
  • Author(s): 竹内美緒・丸茂克美・前田広人・根建宗具・大島健志朗・片山泰樹・山岸昂夫・岩崎渉・鎌形洋一・花田智・玉木秀幸・服部正平・諏訪裕一・坂田将
  • Organizer: 第２９回日本微生物生態学会
  • Place of Presentation: 鹿児島大学
• [Book] Bergey’s Manual of Systematics of Archaea and Bacteria (2016)
  • Author(s): Mio Takeuchi
  • Total Pages: 2
  • Publisher: John Wiley
• [Book] Bergey’s Manual of Systematics of Archaea and Bacteria (BMSAB) (online) (2015)
  • Author(s): Mio Takeuchi
  • Publisher: Wiley